scholarly journals 3D mapping of nanoscale crosslink heterogeneities in microgels

2018 ◽  
Vol 5 (6) ◽  
pp. 1130-1136 ◽  
Author(s):  
Apostolos A. Karanastasis ◽  
Yongdeng Zhang ◽  
Gopal S. Kenath ◽  
Mark D. Lessard ◽  
Joerg Bewersdorf ◽  
...  
Keyword(s):  
Crosslink Density ◽  
Super Resolution ◽  
Spatial Variations ◽  
3D Mapping ◽  
Cross Link ◽  
Nanoscale Features ◽  
Super Resolution Microscopy ◽  
Microscopy Images

The majority of gels exhibit nanoscale spatial variations in crosslink density. We present the first 3D super-resolution microscopy images of dye tagged cross-link distributions in microgels and hydrogels. The morphology of nanoscale features never imaged previously in microgels, are revealed.

scholarly journals Correlation Functions Provide a Universal Framework for Quantitative Analysis of Localization-Based Super-Resolution Microscopy Images

2014 ◽  
Vol 106 (2) ◽  
pp. 25a
Author(s):  
Joerg Schnitzbauer ◽  
Xiaoyu Shi ◽  
Robert Kasper ◽  
Baohui Chen ◽  
Shijie Zhao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Correlation Functions ◽  
Super Resolution ◽  
Super Resolution Microscopy ◽  
Microscopy Images

scholarly journals A Method for Quantifying Molecular Interactions Using Stochastic Modelling and Super-Resolution Microscopy

2017 ◽  
Author(s):  
Keria Bermudez-Hernandez ◽  
Sarah Keegan ◽  
Donna R. Whelan ◽  
Dylan A. Reid ◽  
Jennifer Zagelbaum ◽  
...  
Keyword(s):  
Super Resolution ◽  
Stochastic Modelling ◽  
Analytical Form ◽  
Molecular Clusters ◽  
Interaction Factor ◽  
Cluster Density ◽  
Super Resolution Microscopy ◽  
The Relationship ◽  
Microscopy Images ◽  
Imagej Plugin

AbstractWe introduce the Interaction Factor (IF), a measure for quantifying the interaction of molecular clusters in super-resolution microscopy images. The IF is robust in the sense that it is independent of cluster density, and it only depends on the extent of the pair-wise interaction between different types of molecular clusters in the image. The IF for a single or a collection of images is estimated by first using stochastic modelling where the locations of clusters in the images are repeatedly randomized to estimate the distribution of the overlaps between the clusters in the absence of interaction (IF=0). Second, an analytical form of the relationship between IF and the overlap (which has the random overlap as its only parameter) is used to estimate the IF for the experimentally observed overlap. The advantage of IF compared to conventional methods to quantify interaction in microscopy images is that it is insensitive to changing cluster density and is an absolute measure of interaction, making the interpretation of experiments easier. We validate the IF method by using both simulated and experimental data and provide an ImageJ plugin for determining the IF of an image.

Quantification of functional crosslinker reaction kinetics via super-resolution microscopy of swollen microgels

Soft Matter ◽  
2019 ◽  
Vol 15 (45) ◽  
pp. 9336-9342 ◽  
Author(s):  
Apostolos A. Karanastasis ◽  
Gopal S. Kenath ◽  
Ravishankar Sundararaman ◽  
Chaitanya K. Ullal
Keyword(s):  
Reaction Kinetics ◽  
Rate Constants ◽  
Crosslink Density ◽  
Super Resolution ◽  
Density Distributions ◽  
Super Resolution Microscopy

Super resolution microscopy of microgels is combined with volume evolution data to calculate rate constants of novel dye tagged crosslinkers, which will be useful in predicting crosslink density distributions.

Accurate single-molecule localization of super-resolution microscopy images using multiscale products

2012 ◽  
Author(s):  
Viet Anh Ngo ◽  
Yan Nei Law ◽  
Srivats Hariharan ◽  
Hwee Kuan Lee ◽  
Sohail Ahmed
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Multiscale Products ◽  
Super Resolution Microscopy ◽  
Microscopy Images

scholarly journals Effect of labeling density and time post labeling on quality of antibody-based super resolution microscopy images

2015 ◽  
Author(s):  
Amy M. Bittel ◽  
Isaac Saldivar ◽  
Nicholas Dolman ◽  
Andrew K. Nickerson ◽  
Li-Jung Lin ◽  
...  
Keyword(s):  
Super Resolution ◽  
Super Resolution Microscopy ◽  
Microscopy Images

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy
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