A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering

2018 ◽  
Vol 5 (6) ◽  
pp. 1100-1111 ◽  
Author(s):  
Rúben F. Pereira ◽  
Aureliana Sousa ◽  
Cristina C. Barrias ◽  
Paulo J. Bártolo ◽  
Pedro L. Granja
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
De Novo ◽  
Single Component ◽  
Biophysical Properties ◽  
Dermal Tissue ◽  
Extracellular Matrix Components ◽  
Matrix Components

Bioprinted dual-crosslinked 3D constructs with tunable biochemical and biophysical properties guide the de novo deposition of extracellular matrix components of dermal tissue.

scholarly journals A Strategy to Enhance Secretion of Extracellular Matrix Components by Stem Cells: Relevance to Tissue Engineering

2018 ◽  
Vol 24 (1-2) ◽  
pp. 145-156 ◽  
Author(s):  
Navaneethakrishnan Krishnamoorthy ◽  
Yuan‐Tsan Tseng ◽  
Poornima Gajendrarao ◽  
Padmini Sarathchandra ◽  
Ann McCormack ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Extracellular Matrix Components ◽  
Matrix Components

scholarly journals Comparison of two methods for prolong storage of decellularized mouse whole testis for tissue engineering application: An experimental study

2021 ◽  
Author(s):  
Nasrin Majidi Gharenaz ◽  
Mansoureh Movahedin ◽  
Zohreh Mazaheri
Keyword(s):  
Tissue Engineering ◽  
Experimental Study ◽  
Extracellular Matrix ◽  
Mouse Embryonic Fibroblast ◽  
Slow Freezing ◽  
Histological Evaluation ◽  
Biological Scaffolds ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Embryonic Fibroblast

Background: Biological scaffolds are derived by the decellularization of tissues or organs. Various biological scaffolds, such as scaffolds for the liver, lung, esophagus, dermis, and human testicles, have been produced. Their application in tissue engineering has created the need for cryopreservation processes to store these scaffolds. Objective: The aim was to compare the two methods for prolong storage testicular scaffolds. Materials and Methods: In this experimental study, 20 male NMRI mice (8 wk) were sacrificed and their testes were removed and treated with 0.5% sodium dodecyl sulfate followed by Triton X-100 0.5%. The efficiency of decellularization was determined by histology and DNA quantification. Testicular scaffolds were stored in phosphate-buffered saline solution at 4ºC or cryopreserved by programmed slow freezing followed by storage in liquid nitrogen. Masson’s trichrome staining, Alcian blue staining and immunohistochemistry, collagen assay, and glycosaminoglycan assay were done prior to and after six months of storage under each condition. Results: Hematoxylin-eosin staining showed no remnant cells after the completion of decellularization. DNA content analysis indicated that approximately 98% of the DNA was removed from the tissue (p = 0.02). Histological evaluation confirmed the preservation of extracellular matrix components in the fresh and frozen-thawed scaffolds. Extracellular matrix components were decreased by 4ºC-stored scaffolds. Cytotoxicity tests with mouse embryonic fibroblast showed that the scaffolds were biocompatible and did not have a harmful effect on the proliferation of mouse embryonic fibroblast cells. Conclusion: Our results demonstrated the superiority of the slow freezing method for prolong storage of testicular scaffolds. Key words: Cryopreservation, Testis, Scaffold, Mouse. 

Tissue Fabrication: Reconstitution and Remodeling in Vitro

1991 ◽  
Vol 252 ◽  
Author(s):  
Eugene Bell ◽  
Sumi Scott
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Collagen Matrix ◽  
Dermal Fibroblasts ◽  
Matrix Remodeling ◽  
Dermal Tissue ◽  
Extracellular Matrix Components ◽  
Host Tissues

ABSTRACTTwo approaches to the reconstitution of tissues and organs are reviewed. The first consists of imitating the architecture of actual tissues and organs by combining cultured specialized cells with extracellular matrix components to produce a connective tissue substrate on or in which epithelial, mesothelial or endothelial cells can be plated or seeded and subsequently differentiate into mono or multilayered tissues and other structures. The second consists of providing an acellular framework of extracellular matrix constituents that can be occupied by adjacent host tissues after implantation in vivo and be remodeled by them to resemble the host tissues it is designed to replace. A paradigm for events in vivo, designed to study the process of remodeling of acellular matrices in vitro has been developed. The living skin equivalent (LSE), an example of a product fabricated using the first approach to tissue engineering, has been adapted to study events of extracellular matrix remodeling, relevent to the second approach to tissue engineering. After creating a disc shaped wound bed in an LSE, the wound is filled with a collagen matrix with or without added supplements and the process of epidermal wound closure and associated events in the dermis are followed. It is shown that fibroblast conditioned medium or a simple molecule such as ascorbic acid, added with no additional growth factors to the collagen matrix used to fill the wound bed, strongly stimulates the process of repair. Dermal fibroblasts from the adjacent tissue invade the collagen lattice that forms in the wound bed, and keratinocytes recruited from the wound edge overgrow the new dermal tissue. The applicability of the paradigm to the repair of vascular and other tissues will be discussed and approaches to optimizing the composition of acellular constructs considered.

scholarly journals In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1692 ◽  
Author(s):  
Cristina Blanco-Elices ◽  
Enrique España-Guerrero ◽  
Miguel Mateu-Sanz ◽  
David Sánchez-Porras ◽  
Óscar García-García ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Oral Mucosa ◽  
Dental Tissues ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Time Required ◽  
Vitro Development

Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development.

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