Two-level submicron high porosity membranes (2LHPM) for the capture and release of white blood cells (WBCs)

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 589-597 ◽  
Author(s):  
Javier Alejandro Hernández-Castro ◽  
Kebin Li ◽  
Jamal Daoud ◽  
David Juncker ◽  
Teodor Veres
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
High Porosity ◽  
Capture And Release ◽  
Micro Molding

A method modifying a vacuum-assisted UV micro-molding (VAUM) process is proposed for the fabrication of polymer two-level submicron high porosity membranes (2LHPM).

The Use of Schwartz Geometries for Scaffold Design in Tissue Engineering Applications

2010 ◽  
Author(s):  
Henrique A. Almeida ◽  
Paulo J. Ba´rtolo
Keyword(s):  
Tissue Engineering ◽  
Minimal Surfaces ◽  
Blood Cells ◽  
High Surface ◽  
White Blood Cells ◽  
Host Tissue ◽  
High Porosity ◽  
Computational Tool ◽  
Engineering Applications ◽  
Structural Compatibility

Additive biomanufacturing processes are increasingly recognised as ideal techniques to produce scaffolds for tissue engineering applications. Scaffolds provide a temporary mechanical and vascular support for tissue regeneration while shaping the in-growth tissues. These scaffolds must be biocompatible, biodegradable, with appropriate porosity, pore structure and pore distribution and optimal vascularisation, with both surface and structural compatibility. Surface compatibility means a chemical, biological and physical suitability to the host tissue. Structural compatibility corresponds to an optimal adaptation to the mechanical behaviour of the host tissue. Recent advances in the tissue engineering field are increasingly relying on modelling and simulation. The design of optimised scaffolds based on the fundamental knowledge of its microstructure is a relevant topic of research. This paper proposes the use of novel geometric structures based on the Triple Periodic Minimal Surfaces formulation, namely the Schwartz primitives, one of the a sub-classes of Triple Periodic Minimal Surfaces. Schwartz primitives enables the design of vary high surface-to-volume ratio structures with high porosity and mechanical/vascular properties. With the use of a computational tool combining structural, computational fluid dynamics and topological optimisation schemes, it is possible to predict and optimise both mechanical and vascular behaviour of scaffolds for soft and hard tissue applications, with different topological architectures and levels of porosity. This tool is particularly important to quantify the structural heterogeneity and scaffold mechanical properties with a designed microstructure subjected to either a single or a multiple load distribution. This computational tool enables the simulation of biological flows in vascular passages of scaffolds. The blood flow considered in this study is a complex fluid comprising a suspension of red blood cells, white blood cells and platelets within a newtonian plasma.

Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

1991 ◽  
Vol 49 ◽  
pp. 104-105
Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar
Keyword(s):  
Medical Devices ◽  
Blood Cells ◽  
White Blood Cells ◽  
Consumer Products ◽  
Ultrastructural Changes ◽  
Ultraviolet A ◽  
Uv Spectrum ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

The Effect of Red Blood Cells on the ADP-induced Aggregation of Human Platelets in Flow Through Tubes

1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith
Keyword(s):  
Platelet Aggregation ◽  
Shear Rate ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Mean Transit Time ◽  
White Blood Cells ◽  
Aggregate Size ◽  
Human Platelets ◽  
Induced Aggregation

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.

White blood cells levels and PCOS: direct and indirect relationship with insulin resistance, but not with hyperandogenemia

2013 ◽  
Author(s):  
Olga Papalou ◽  
Sarantis Livadas ◽  
Athanasios Karachalios ◽  
Nektarios Benetatos ◽  
George Boutzios ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Blood Cells ◽  
White Blood Cells ◽  
Indirect Relationship

Endogenous Heparinoids Detected by anti-Xa Activity are Present in Blood during Acute Variceal Bleeding in Cirrhosis. A Prospective Study

2014 ◽  
Vol 23 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Christos Triantos ◽  
Emmanuel Louvros ◽  
Maria Kalafateli ◽  
Anne Riddell ◽  
Ulrich Thalheimer ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Variceal Bleeding ◽  
Blood Cells ◽  
Venous Pressure ◽  
White Blood Cells ◽  
International Normalized Ratio ◽  
Ulcer Bleeding ◽  
Factor X ◽  
Packed Red Blood Cells ◽  
Bacterial Peritonitis

Background & Aims: Endogenous heparinoids have been detected by thromboelastography and quantified by clotting based anti-Xa activity assays in patients with cirrhosis, but their presence in variceal bleeding has not been established yet.Methods: Clotting based anti-Xa activity was measured in A) 30 cirrhotics with variceal bleeding, B) 15 noncirrhotics with peptic ulcer bleeding, C) 10 cirrhotics without infection or bleeding, and D) 10 cirrhotics with hepatocellular carcinoma (HCC).Results: Anti-Xa activity was not detected in ulcer bleeders or in cirrhotics without infection or bleedingbut was present in seven (23%) variceal bleeders (median levels: 0.03 u/mL (0.01-0.07)) and was quantifiable for 3 days in six of seven patients. Four of seven variceal bleeders with anti-Xa activity present had HCC (p=0.023). Age, creatinine, platelet count and total infections the second day from admission were significantly correlated with the presence of measureable anti-Xa levels (p=0.014, 0.032, 0.004 and 0.019, respectively). In the HCC group, anti-Xa activity was present in three patients (30%) [median levels: 0.05 u/mL (0.01-0.06)].Conclusions: In this study, variceal bleeders and 30% of the patients with HCC had endogenous heparinoids that were detected by a clotting based anti-Xa activity assay, whereas there was no anti Xa activity present in patients with cirrhosis without infection, or bleeding or HCC, nor in those with ulcer bleeding. Thus, the anti-Xa activity is likely to be a response to bacterial infection and/or presence of HCC in cirrhosis.List of abbreviations: AFP, alpha-fetoprotein; aPTT, activated partial thromboplastin time; CP, Child-Pugh; FXa, activated factor X; GAGS, glycosaminoglycans; Hb, hemoglobin; HCC, hepatocellular carcinoma; HVPG, hepatic venous pressure gradient; INR, International normalized ratio; LMWHs, low molecular weight heparins; MELD, Model for End-stage Liver Disease; PPP, platelet-poor plasma; PRBC, packed red blood cells; PT, prothrombin time; SBP, sponataneous bacterial peritonitis; TEG, thromboelastography; WBC, white blood cells.

Gene Expression Differences in White Blood Cells after Escherichia coli Infection in Chickens

2012 ◽  
Author(s):  
Erin Sandford ◽  
Megan Orr ◽  
Xianyao Li ◽  
Huaijun Zhou ◽  
timothy J. Johnson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Blood Cells ◽  
White Blood Cells ◽  
Escherichia Coli Infection

DETERMINATION OF WHITE BLOOD CELLS USING FOLDSCOPE WITH SMARTPHONE

2019 ◽  
pp. 10-13
Author(s):  
Ranu Kumar ◽  
Prasad Kapildeo
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Healthcare Services ◽  
Field Level ◽  
Morphology Analysis

We are traditionally used Microscope in clinical laboratory for determination of white blood cells of human blood smear. Now, in this study we were used Foldscope with Smartphone in the place of Microscope and examine many samples of human blood smear which was collected from local diagnostic centers. We were very easily quantity & morphology analysis of all types of WBC cells such as Neutrophils, Lymphocytes, Monocytes, Eosionophils, Basophils in blood smear with the help of Foldscope & image taken by Smartphone. The main objective of this study is to use Foldscope for quantity & morphology analysis of human WBCs at field level especially poor resource area where healthcare services or centers is not available & where carry of microscope is not possible.

PENENTUAN KRITERIA PENILAIAN KESAN JUMLAH LEUKOSIT PADA PEMERIKSAAN APUSAN DARAH TEPI

2018 ◽  
Vol 3 (2) ◽  
pp. 52-61
Author(s):  
Dzikra Arwie ◽  
Islawati
Keyword(s):  
Data Analysis ◽  
Statistical Analysis ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Field Of View ◽  
Laboratory Observation ◽  
Number Of Cells

Leukocytes or white blood cells have a characteristic characteristic of different cells. Determination of the impression of the number of leukocytes is determined in the number of cells in the field of view. While the number of viewable field cells expressed is still quite varied. The purpose of this study was to determine the number of leukocytes in the field of view and expressed the impression of a sufficient amount. This research was conducted at the Laboratory of Health Analyst Department Panrita Husada Bulukumba on 9 April 2017 to 14 July 2017. This type of research is a laboratory observation that aims to determine the criteria for assessing the impression of the number of leukocytes on a peripheral blood smear. Data analysis using statistical analysis is the average and standard deviations to determine the impression of the number of leukocytes and use 3 inspection zones. The results of this study obtained results in zone IV the number of leukocyte impressions said to be sufficient was 7-10, in zone V the number of leukocyte impressions said to be sufficient was 4-9, and in zone VI the number of leukocyte impressions said to be sufficient was 3-8.  

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