scholarly journals Ultrasensitive quantification of tumor mRNAs in extracellular vesicles with an integrated microfluidic digital analysis chip

Lab on a Chip ◽  
2018 ◽  
Vol 18 (24) ◽  
pp. 3790-3801 ◽  
Author(s):  
Peng Zhang ◽  
Jennifer Crow ◽  
Divya Lella ◽  
Xin Zhou ◽  
Glenson Samuel ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Single Molecule ◽  
Ewing Sarcoma ◽  
Liquid Biopsy ◽  
Single Molecule Detection ◽  
Digital Analysis ◽  
Fusion Transcripts ◽  
Non Invasive ◽  
Mrna Analysis ◽  
Analysis Platform

A microwell-patterned microfluidic digital mRNA analysis platform enables PCR-free, single-molecule detection of EWS-FLI1 fusion transcripts in EVs towards liquid biopsy-based non-invasive diagnosis of Ewing Sarcoma.

scholarly journals Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer

2020 ◽  
Vol 9 (1) ◽  
pp. 1809765 ◽  
Author(s):  
Ping Wei ◽  
Fei Wu ◽  
Bin Kang ◽  
Xiaohua Sun ◽  
Fabienne Heskia ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Extracellular Vesicles ◽  
Single Molecule ◽  
Liquid Biopsy ◽  
Array Technology

scholarly journals Extracellular Vesicles from Thyroid Carcinoma: The New Frontier of Liquid Biopsy

2019 ◽  
Vol 20 (5) ◽  
pp. 1114 ◽  
Author(s):  
Germana Rappa ◽  
Caterina Puglisi ◽  
Mark Santos ◽  
Stefano Forte ◽  
Lorenzo Memeo ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Thyroid Nodules ◽  
Endocrine System ◽  
Diagnostic Procedures ◽  
Lymph Node Involvement ◽  
Phospholipid Bilayer ◽  
Invasive Approach ◽  
Non Invasive

The diagnostic approach to thyroid cancer is one of the most challenging issues in oncology of the endocrine system because of its high incidence (3.8% of all new cancer cases in the US) and the difficulty to distinguish benign from malignant non-functional thyroid nodules and establish the cervical lymph node involvement during staging. Routine diagnosis of thyroid nodules usually relies on a fine-needle aspirate biopsy, which is invasive and often inaccurate. Therefore, there is an urgent need to identify novel, accurate, and non-invasive diagnostic procedures. Liquid biopsy, as a non-invasive approach for the detection of diagnostic biomarkers for early tumor diagnosis, prognosis, and disease monitoring, may be of particular benefit in this context. Extracellular vesicles (EVs) are a consistent source of tumor-derived RNA due to their prevalence in circulating bodily fluids, the well-established isolation protocols, and the fact that RNA in phospholipid bilayer-enclosed vesicles is protected from blood-borne RNases. Recent results in other types of cancer, including our recent study on plasma EVs from glioblastoma patients suggest that information derived from analysis of EVs from peripheral blood plasma can be integrated in the routine diagnostic tumor approach. In this review, we will examine the diagnostic and prognostic potential of liquid biopsy to detect tumor-derived nucleic acids in circulating EVs from patients with thyroid carcinoma.

scholarly journals Opportunities and Challenges of Liquid Biopsy in Thyroid Cancer

2021 ◽  
Vol 22 (14) ◽  
pp. 7707
Author(s):  
Chiara Romano ◽  
Federica Martorana ◽  
Maria Stella Pennisi ◽  
Stefania Stella ◽  
Michele Massimino ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Nucleic Acids ◽  
Tumor Cells ◽  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Endocrine System ◽  
Invasive Approach ◽  
Medical Needs ◽  
Non Invasive ◽  
Thyroid Malignancies

Thyroid cancer is the most common malignancy of the endocrine system, encompassing different entities with distinct histological features and clinical behavior. The diagnostic definition, therapeutic approach, and follow-up of thyroid cancers display some controversial aspects that represent unmet medical needs. Liquid biopsy is a non-invasive approach that detects and analyzes biological samples released from the tumor into the bloodstream. With the use of different technologies, tumor cells, free nucleic acids, and extracellular vesicles can be retrieved in the serum of cancer patients and valuable molecular information can be obtained. Recently, a growing body of evidence is accumulating concerning the use of liquid biopsy in thyroid cancer, as it can be exploited to define a patient’s diagnosis, estimate their prognosis, and monitor tumor recurrence or treatment response. Indeed, liquid biopsy can be a valuable tool to overcome the limits of conventional management of thyroid malignancies. In this review, we summarize currently available data about liquid biopsy in differentiated, poorly differentiated/anaplastic, and medullary thyroid cancer, focusing on circulating tumor cells, circulating free nucleic acids, and extracellular vesicles.

scholarly journals Fusion FISH Imaging: Single-Molecule Detection of Gene Fusion Transcripts In Situ

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e93488 ◽  
Author(s):  
Fatu Badiane Markey ◽  
William Ruezinsky ◽  
Sanjay Tyagi ◽  
Mona Batish
Keyword(s):  
Single Molecule ◽  
Gene Fusion ◽  
Single Molecule Detection ◽  
Fusion Transcripts

scholarly journals Bacteria-Derived Extracellular Vesicles in Urine as a Novel Biomarker for Gastric Cancer: Integration of Liquid Biopsy and Metagenome Analysis

Cancers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 4687
Author(s):  
Jae-Yong Park ◽  
Chil-Sung Kang ◽  
Ho-Chan Seo ◽  
Jin-Chul Shin ◽  
Sung-Min Kym ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Prediction Models ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Metagenome Analysis ◽  
Non Invasive ◽  
Genus Level ◽  
Relative Abundances

Early detection is crucial for improving the prognosis of gastric cancer, but there are no non-invasive markers for the early diagnosis of gastric cancer in real clinical settings. Recently, bacteria-derived extracellular vesicles (EVs) emerged as new biomarker resources. We aimed to evaluate the microbial composition in gastric cancer using bacteria-derived EVs and to build a diagnostic prediction model for gastric cancer with the metagenome data. Stool, urine, and serum samples were prospectively collected from 453 subjects (gastric cancer, 181; control, 272). EV portions were extracted from the samples for metagenome analysis. Differences in microbial diversity and composition were analyzed with 16S rRNA gene profiling, using the next-generation sequencing method. Biomarkers were selected using logistic regression models based on relative abundances at the genus level. The microbial composition of healthy groups and gastric cancer patient groups was significantly different in all sample types. The compositional differences of various bacteria, based on relative abundances, were identified at the genus level. Among the diagnostic prediction models for gastric cancer, the urine-based model showed the highest performance when compared to that of stool or serum. We suggest that bacteria-derived EVs in urine can be used as novel metagenomic markers for the non-invasive diagnosis of gastric cancer by integrating the liquid biopsy method and metagenome analysis.

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

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