scholarly journals Dual-phone illumination-imaging system for high resolution and large field of view multi-modal microscopy

Lab on a Chip ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 825-836 ◽  
Author(s):  
Sara Kheireddine ◽  
Ayyappasamy Sudalaiyadum Perumal ◽  
Zachary J. Smith ◽  
Dan V. Nicolau ◽  
Sebastian Wachsmann-Hogiu

Bright-field, dark-field, Rheinberg, fluorescence microscopy on a mobile phone with phone screen illumination.

2014 ◽  
Vol 898 ◽  
pp. 614-617
Author(s):  
Rui Hong Li ◽  
Yue Ping Han

The present paper reviews the X-ray grating imaging systems at home and abroad from the aspects of technological characterizations and the newest researching focus. First, not only the imaging principles and the frameworks of the typical X-ray grating imaging system based on Talbot-Lau interferometry method, but also the algorithms of retrieving the signals of attenuation, refraction and small-angle scattering are introduced. Second, the system optimizing methods are discussed, which involves mainly the relaxing the requirement of high positioning resolution and strict circumstances for gratings and designing large field of view with high resolution. Third, two and four-dimensional grating-based X-ray imaging techniques are introduced.


2020 ◽  
Author(s):  
Ruiqing Ni ◽  
Zhenyue Chen ◽  
Juan A. Gerez ◽  
Gloria Shi ◽  
Quanyu Zhou ◽  
...  

AbstractCurrent intravital microscopy techniques visualize tauopathy with high-resolution, but have a small field-of-view and depth-of-focus. Herein, we report a transcranial detection of tauopathy over the entire cortex of P301L tauopathy mice using large-field multifocal illumination (LMI) fluorescence microscopy technique and luminescent conjugated oligothiophenes. In vitro assays revealed that fluorescent ligand h-FTAA is optimal for in vivo tau imaging, which was confirmed by observing elevated probe retention in the cortex of P301L mice compared to non-transgenic littermates. Immunohistochemical staining further verified the specificity of h-FTAA to detect tauopathy in P301L mice. The new imaging platform can be leveraged in pre-clinical mechanistic studies of tau spreading and clearance as well as longitudinal monitoring of tau targeting therapeutics.


2016 ◽  
Author(s):  
Hanglin Cheng ◽  
Qun Hao ◽  
Yao Hu ◽  
Jie Cao ◽  
Shaopu Wang ◽  
...  

2021 ◽  
Vol 41 (2) ◽  
pp. 0208002
Author(s):  
李江勇 Li Jiangyong ◽  
冯位欣 Feng Weixin ◽  
刘飞 Liu Fei ◽  
魏雅喆 Wei Yazhe ◽  
邵晓鹏 Shao Xiaopeng

2020 ◽  
Vol 49 (8) ◽  
pp. 20200005-20200005
Author(s):  
陈炳旭 Bingxu Chen ◽  
廖志远 Zhiyuan Liao ◽  
操超 Chao Cao ◽  
白瑜 Yu Bai ◽  
牟达 Da Mu

2021 ◽  
Author(s):  
Ruixiao Li ◽  
Zeuku Ho ◽  
Xiaodong Gu ◽  
Satoshi Shinada ◽  
Fumio Koyama

2011 ◽  
Author(s):  
W. Li ◽  
J. Gelb ◽  
Y. Yang ◽  
Y. Guan ◽  
W. Wu ◽  
...  

2001 ◽  
Vol 19 (2) ◽  
pp. 285-293 ◽  
Author(s):  
T.A. PIKUZ ◽  
A. YA. FAENOV ◽  
M. FRAENKEL ◽  
A. ZIGLER ◽  
F. FLORA ◽  
...  

The shadow monochromatic backlighting (SMB) scheme, a modification of the well-known soft X-ray monochromatic backlighting scheme, is proposed. It is based on a spherical crystal as the dispersive element and extends the traditional scheme by allowing one to work with a wide range of Bragg angles and thus in a wide spectral range. The advantages of the new scheme are demonstrated experimentally and supported numerically by ray-tracing simulations. In the experiments, the X-ray backlighter source is a laser-produced plasma, created by the interaction of an ultrashort pulse, Ti:Sapphire laser (120 fs, 3–5 mJ, 1016 W/cm2 on target) or a short wavelength XeCl laser (10 ns, 1–2 J, 1013 W/cm2 on target) with various solid targets (Dy, Ni + Cr, BaF2). In both experiments, the X-ray sources are well localized spatially (∼20 μm) and are spectrally tunable in a relatively wide wavelength range (λ = 8–15 Å). High quality monochromatic (δλ/λ ∼ 10−5–10−3) images with high spatial resolution (up to ∼4 μm) over a large field of view (a few square millimeters) were obtained. Utilization of spherically bent crystals to obtain high-resolution, large field, monochromatic images in a wide range of Bragg angles (35° < Θ < 90°) is demonstrated for the first time.


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