Microfluidic affinity separation chip for selective capture and release of label-free ovarian cancer exosomes

Lab on a Chip ◽  
2018 ◽  
Vol 18 (20) ◽  
pp. 3144-3153 ◽  
Author(s):  
Colin L. Hisey ◽  
Kalpana Deepa Priya Dorayappan ◽  
David E. Cohn ◽  
Karuppaiyah Selvendiran ◽  
Derek J. Hansford
Keyword(s):  
Ovarian Cancer ◽  
Microfluidic Device ◽  
Label Free ◽  
Affinity Separation ◽  
Capture And Release

An elution technique selectively captures and releases intact, label-free exosomes from a microfluidic device for characterizing ovarian cancer serum.

scholarly journals Label-Free Virus Capture and Release by a Microfluidic Device Integrated with Porous Silicon Nanowire Forest

Small ◽  
2016 ◽  
Vol 13 (6) ◽  
pp. 1603135 ◽  
Author(s):  
Yiqiu Xia ◽  
Yi Tang ◽  
Xu Yu ◽  
Yuan Wan ◽  
Yizhu Chen ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Microfluidic Device ◽  
Silicon Nanowire ◽  
Label Free ◽  
Free Virus ◽  
Capture And Release ◽  
Porous Silicon Nanowire

scholarly journals Circulating tumor cells enumerated by a centrifugal microfluidic device as a predictive marker for treatment monitoring of ovarian cancer patients

2019 ◽  
Author(s):  
hyera kim ◽  
Minji Lim ◽  
Jin Young Kim ◽  
So-Jin Shin ◽  
Yoon-Kyoung Cho ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Predictive Value ◽  
Clinical Status ◽  
Predictive Marker ◽  
Multiple Time ◽  
Label Free

Abstract Background: We investigated the size-based isolation and enumeration of circulating tumor cells (CTCs) using a centrifugal microfluidic device equipped with fluid-assisted separation technology (FAST disc) and demonstrated the correlation among CTC count, CA125, and the clinical course. Methods: We prospectively recruited 13 women with ovarian cancer between December 2016 and August 2018 at Keimyung University Dongsan Hospital. We collected 49 serial blood samples at multiple time-points. CTCs were isolated from whole blood using the FAST disc and were defined as EpCAM+, cytokeratin+, CD45−, and DAPI+. Results: We successfully achieved the high-throughput, efficient, and label-free isolation of CTCs from the blood of ovarian cancer patients using FAST discs. The mean and median CTC count were 20.2 and 6.0, respectively, and 84.62% patients (11/13) had more than 1 CTC at baseline and a decreased CTC count after surgery and chemotherapy, except for 2 patients who had no CTCs at baseline. The median follow-up duration was 22.7 months. At the time of complete response, CTC count in 8 patients was <3. CTC count was correlated with CA125 in 3 patients with no recurrence but elevated in 3 patients with recurrence and normal range of CA125. CTC count and CA125 had high concordance with directional change (increasing 71.4% and decreasing 75.0%). CTC count showed higher association with the clinical status and higher sensitivity (100.0% vs. 60.0%), positive predictive value (55.6% vs. 42.9%), and negative predictive value (100.0% vs. 87.5%) than CA125.Conclusions: CTC count was better associated with treatment response and recurrence than CA125.

A microfluidic device with integrated impedance detection for λ-DNA

2003 ◽  
Vol 773 ◽  
Author(s):  
Myung-Il Park ◽  
Jonging Hong ◽  
Dae Sung Yoon ◽  
Chong-Ook Park ◽  
Geunbae Im
Keyword(s):  
Microfluidic Device ◽  
Electrochemical Impedance ◽  
Direct Detection ◽  
Full Potential ◽  
Label Free ◽  
Buffer Solution ◽  
Detection Systems ◽  
Significant Difference ◽  
Detection Of Biomolecules ◽  
Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

scholarly journals Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics

Polymers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 267
Author(s):  
Chen Jiao ◽  
Franziska Obst ◽  
Martin Geisler ◽  
Yunjiao Che ◽  
Andreas Richter ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Disulfide Bonds ◽  
Stimuli Responsive ◽  
Disulfide Exchange ◽  
Protein Capture ◽  
Wide Range ◽  
Redox Responsive ◽  
Potential Applications ◽  
Capture And Release ◽  
Cross Linker

Stimuli-responsive hydrogels have a wide range of potential applications in microfluidics, which has drawn great attention. Double cross-linked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we report the integration of poly(N-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent cross-linker (N,N′-methylenebisacrylamide, BIS) and a redox responsive reversible cross-linker (N,N′-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization. Cleavage and re-formation of disulfide bonds introduced by BAC changed the cross-linking densities of the hydrogel dots, making them swell or shrink. Rheological measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)molecules (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery.

Identifying Potential Markers for Monitoring Progression to Ovarian Cancer Using Plasma Label-free Proteomics

2019 ◽  
Author(s):  
Wenjie Wang ◽  
Hongyu Xie ◽  
Bairong Xia ◽  
LiuChao Zhang ◽  
Ce Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Patients ◽  
Plasma Protein ◽  
Late Stage ◽  
Early Stage ◽  
Label Free ◽  
Absolute Quantitation ◽  
Keywords Ovarian Cancer ◽  
Benign Ovarian Tumor ◽  
Increased Risk

Abstract PurposeCancer antigen 125 (CA125) is considered to have high sensitivity but poor specificity for ovarian cancer. New biomarkers utilized to early detect and monitor the progression of ovarian cancer patients are critically needed. Methods A total of 80 patients including 16 early stage, and matched with 17 late stage, 23 benign ovarian tumor (BOT) and 24 uterine fibroid (UF) patients were utilized to perform plasma proteomics analysis using isobaric tag for relative and absolute quantitation (iTRAQ) method to identify differential diagnostic proteins of ovarian cancer patients. A validation set of 9 early stage, 11 late stage, 17 BOT and 16 UF collected by an independent cohort of samples with the same matching principles was examined to confirm the expressed levels of differential expression proteins by ELISA analysis. Results CRP and ARHGEF 11 were identified as potential diagnostic biomarkers of ovarian cancer. Results of area under the curve (AUC) analysis suggested that combination of diagnostic proteins and CA125 achieved a much higher diagnostic accuracy compared with CA125 alone (AUC values: 0.98 versus 0.80), especially improved the specificity (0.97 versus 0.77). In addition, elevated plasma CRP levels were associated with increased risk of ovarian cancer. Conclusions Current study found that plasma protein CRP was an indicator for monitoring the progression of ovarian cancer. Combination of plasma protein biomarkers with CA125 could be utilized to early diagnose of ovarian cancer patients. Keywords ovarian cancer, proteomics, diagnosis, progression, CRP

scholarly journals Multi-layer assembly of cellulose nanofibrils in a microfluidic device for the selective capture and release of viable tumor cells from whole blood

Nanoscale ◽  
2020 ◽  
Vol 12 (42) ◽  
pp. 21788-21797
Author(s):  
Tharagan Kumar ◽  
Ruben R. G. Soares ◽  
Leyla Ali Dholey ◽  
Harisha Ramachandraiah ◽  
Negar Abbasi Aval ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Microfluidic Device ◽  
Whole Blood ◽  
Cellulose Nanofibrils ◽  
Layer By Layer ◽  
Layer By Layer Assembly ◽  
Viable Tumor ◽  
Capture And Release ◽  
Layer Assembly

A microfluidic device modified with a layer-by-layer assembly of cellulose nanofibrils allows efficient capture and enzymatic release of tumor cells.

scholarly journals Magnetic Control of an Electrochemical Microfluidic Device with an Arrayed Immunosensor for Simultaneous Multiple Immunoassays

2007 ◽  
Vol 53 (7) ◽  
pp. 1323-1329 ◽  
Author(s):  
Dianping Tang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Tumor Markers ◽  
Microfluidic Device ◽  
Simultaneous Detection ◽  
Reference Electrode ◽  
Cost Effective ◽  
Sio2 Nanoparticles ◽  
Magnetic Bead ◽  
Label Free ◽  
Specific Tumor ◽  
Protein Diagnostics

Abstract Background: Methods based on magnetic bead probes have been developed for immunoassay, but most involve complicated labeling or stripping procedures and are unsuitable for routine use. Methods: We synthesized magnet core/shell NiFe2O4/SiO2 nanoparticles and fabricated an electrochemical magnetic controlled microfluidic device for the detection of 4 tumor markers. The immunoassay system consisted of 5 working electrodes and an Ag/AgCl reference electrode integrated on a glass substrate. Each working electrode contained a different antibody immobilized on the NiFe2O4/SiO2 nanoparticle surface and was capable of measuring a specific tumor marker using noncompetitive electrochemical immunoassay. Results: Under optimal conditions, the multiplex immunoassay enabled the simultaneous detection of 4 tumor markers. The sensor detection limit was &lt;0.5 μg/L (or &lt;0.5 kunits/L) for most analytes. Intra- and interassay imprecisions (CVs) were &lt;4.5% and 8.7% for analyte concentrations &gt;5 μg/L (or &gt;5 kunits/L), respectively. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross-talk (CV) between neighboring sites was &lt;10%. Conclusion: This immunoassay system offers promise for label-free, rapid, simple, cost-effective analysis of biological samples. Importantly, the chip-based immunosensor could be suitable for use in the mass production of miniaturized lab-on-a-chip devices and open new opportunities for protein diagnostics and biosecurity.

Sign in / Sign up

Export Citation Format

Share Document