Adipose-on-a-chip: a dynamic microphysiological in vitro model of the human adipose for immune-metabolic analysis in type II diabetes

Lab on a Chip ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 241-253 ◽  
Author(s):  
Yunxiao Liu ◽  
Patthara Kongsuphol ◽  
Su Yin Chiam ◽  
Qing Xin Zhang ◽  
Sajay Bhuvanendran Nair Gourikutty ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Immune Cells ◽  
Type Ii Diabetes ◽  
In Vitro Model ◽  
Low Grade ◽  
Type Ii ◽  
Metabolic Analysis ◽  
Vitro Model ◽  
Low Grade Inflammation

Infiltration of immune cells into adipose tissue is associated with chronic low-grade inflammation in obese individuals.

scholarly journals In vitro micro-physiological model of the inflamed human adipose tissue for immune-metabolic analysis in type II diabetes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Patthara Kongsuphol ◽  
Shilpi Gupta ◽  
Yunxiao Liu ◽  
Sajay Bhuvanendran Nair Gourikutty ◽  
Subhra K. Biswas ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Type Ii Diabetes ◽  
Human Adipose Tissue ◽  
Physiological Model ◽  
Type Ii ◽  
Metabolic Analysis

scholarly journals THP-1 Macrophages and SGBS Adipocytes ? A New Human in vitro Model System of Inflamed Adipose Tissue

2011 ◽  
Vol 2 ◽  
Author(s):  
Michaela Keuper ◽  
Anna Dzyakanchuk ◽  
Kurt E. Amrein ◽  
Martin Wabitsch ◽  
Pamela Fischer-Posovszky
Keyword(s):  
Adipose Tissue ◽  
In Vitro Model ◽  
Model System ◽  
In Vitro Model System ◽  
Vitro Model

scholarly journals Adiponectin as Link Factor between Adipose Tissue and Cancer

2019 ◽  
Vol 20 (4) ◽  
pp. 839 ◽  
Author(s):  
Erika Di Zazzo ◽  
Rita Polito ◽  
Silvia Bartollino ◽  
Ersilia Nigro ◽  
Carola Porcile ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Immune Cells ◽  
Active Role ◽  
Low Grade ◽  
Poor Clinical Outcome ◽  
Different Types ◽  
Obesity And Cancer ◽  
Low Grade Inflammation ◽  
Circulating Levels ◽  
Proliferation And Invasion

Adipose tissue is a key regulator of energy balance playing an active role in lipid storage as well as in synthesizing several hormones directly involved in the pathogenesis of obesity. Obesity represents a peculiar risk factor for a growing list of cancers and is frequently associated to poor clinical outcome. The mechanism linking obesity and cancer is not completely understood, but, amongst the major players, there are both chronic low-grade inflammation and deregulation of adipokines secretion. In obesity, the adipose tissue is pervaded by an abnormal number of immune cells that create an inflammatory environment supporting tumor cell proliferation and invasion. Adiponectin (APN), the most abundant adipokine, shows anti-inflammatory, anti-proliferative and pro-apoptotic properties. Circulating levels of APN are drastically decreased in obesity, suggesting that APN may represent the link factor between obesity and cancer risk. The present review describes the recent advances on the involvement of APN and its receptors in the etiology of different types of cancer.

scholarly journals Aerobic training reduces immune cell recruitment and cytokine levels in adipose tissue in obese mice

2019 ◽  
Vol 44 (5) ◽  
pp. 512-520 ◽  
Author(s):  
Débora Romualdo Lacerda ◽  
Michele Macedo Moraes ◽  
Albená Nunes-Silva ◽  
Kátia Anunciação Costa ◽  
Débora Fernandes Rodrigues ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Immune Cells ◽  
Aerobic Training ◽  
Immune Cell ◽  
Control Diet ◽  
Moderate Intensity ◽  
Low Grade ◽  
Carbohydrate Diet ◽  
Cytokine Levels ◽  
Low Grade Inflammation

Obesity is associated with an energy imbalance that results from excessive energy intake, low diet quality, and a sedentary lifestyle. The increased consumption of a high-refined carbohydrate (HC) diet is strongly related to higher adiposity and low-grade inflammation. Aerobic training is a well-known nonpharmacological intervention to treat obesity and metabolic disturbances. However, the mechanisms through which aerobic training ameliorates the low-grade inflammation induced by an HC diet should be further investigated. Our hypothesis herein was that aerobic training would decrease the recruitment of leukocytes in adipose tissue, thereby reducing the levels of cytokines and improving metabolism in mice fed an HC diet. Male Balb/c mice were assigned to the following groups: control diet/nontrained (C-NT), control diet/trained (C-T), high-refined carbohydrate diet/nontrained (HC-NT), and high-refined carbohydrate diet/trained (HC-T). Mice were submitted to moderate-intensity training sessions that consisted of running 60 min per day for 8 weeks. An intravital microscopy technique was performed in vivo in anesthetized mice to visualize the microvasculature of the adipose tissue. The HC diet induced obesity and increased the influx of immune cells into the adipose tissue. In contrast, HC-T mice presented a lower adiposity and adipocyte area. Furthermore, relative to HC-NT mice, HC-T mice showed increased resting energy expenditure, decreased recruitment of immune cells in the adipose tissue, reduced cytokine levels, and ameliorated hyperglycemia and fatty liver deposition. Collectively, our data enhance understanding about the anti-inflammatory effect of aerobic training and shed light on the adipose tissue-mediated mechanisms by which training promotes a healthier metabolic profile.

scholarly journals Obesity and inflammation: reduced cytokine expression due to resveratrol in a human in vitro model of inflamed adipose tissue

2015 ◽  
Vol 6 ◽  
Author(s):  
Ivana Zagotta ◽  
Elitsa Y. Dimova ◽  
Klaus-Michael Debatin ◽  
Martin Wabitsch ◽  
Thomas Kietzmann ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
In Vitro Model ◽  
Cytokine Expression ◽  
Vitro Model

Radiation induced secretion of surfactant from cell cultures of type II pneumocytes: An in vitro model of radiation toxicity

1984 ◽  
Vol 10 (3) ◽  
pp. 375-378 ◽  
Author(s):  
Donald L. Shapiro ◽  
Jacob N. Finkelstein ◽  
Philip Rubin ◽  
David P. Penney ◽  
Dietmar W. Siemann
Keyword(s):  
Cell Cultures ◽  
In Vitro Model ◽  
Radiation Toxicity ◽  
Type Ii ◽  
Type Ii Pneumocytes ◽  
Radiation Induced ◽  
Vitro Model

Adipose tissue stem cell-derived hepatic progenies as an in vitro model for genotoxicity testing

2018 ◽  
Vol 92 (5) ◽  
pp. 1893-1903 ◽  
Author(s):  
Jana Tomc ◽  
Katja Kološa ◽  
Bojana Žegura ◽  
Urška Kamenšek ◽  
Barbara Breznik ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
In Vitro Model ◽  
Vitro Model ◽  
Genotoxicity Testing

scholarly journals Evaluation of low-grade In Vitro Oxidative Stress in Goat Erythrocytes ‎Exposed to Hydrogen Peroxide and Dexamethasone ‎

2021 ◽  
Vol 18 (2) ◽  
pp. 33-37
Author(s):  
M. B. Monguno ◽  
E. S. Philip ◽  
I. C. Uku ◽  
I. O. Igbokwe
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
In Vitro Model ◽  
Sucrose Solution ◽  
Osmotic Fragility ◽  
Low Grade ◽  
Mean Values ◽  
Red Blood Cell Count ◽  
Vitro Model

Under conditions of oxidative stress, erythrocytes of goats could be predisposed to haemolysis. This study was aimed at evaluating the effect of oxidant exposure to goat erythrocytes using an in vitro model. Blood samples from 10 goats were incubated with 0.06 – 0.18 mM hydrogen peroxide (H2O2) either singly or in combination with 0.02 µM dexamethasone for 60 min, and erythrocyte parameters such as packed cell volume (PCV), red blood cell count (RBC), mean corpuscular volume (MCV) and percentage haemolysis in hypotonic sucrose solution (250 mOsmol/L) were determined thereafter. No significant changes in the mean values of all parameters were observed. The in vitro model indicated that erythrocyte parameters remained stable under low-grade oxidant exposure in goats. Therefore, plasma H2O2 concentration of ≤ 0.18 mM, whether in the presence or absence of 0.02 µM dexamethasone, may not induce apparent oxidative damage in goat erythrocytes that could be estimated by PCV, RBC, MCV and sucrose-based osmotic fragility at low hypotonicity.

scholarly journals Physical activity, exercise and low-grade systemic inflammation

2010 ◽  
Vol 69 (3) ◽  
pp. 400-406 ◽  
Author(s):  
Julia Wärnberg ◽  
Karen Cunningham ◽  
Javier Romeo ◽  
Ascension Marcos
Keyword(s):  
Physical Activity ◽  
Type Ii Diabetes ◽  
The Body ◽  
Low Grade ◽  
Type Ii ◽  
Active Lifestyle ◽  
Physically Active ◽  
Inflammatory State ◽  
Physical Activity And Exercise ◽  
Low Grade Inflammation

Prospective studies have shown that chronic low-grade inflammation may contribute to the pathogenesis of the most common chronic diseases and in particular CVD. Obesity has repeatedly been associated with moderately raised levels of inflammation, and this observation has led to the view that obesity is characterised by a state of chronic low-grade inflammation. There is now great interest in elucidating how physical activity and exercise modulate inflammation. This review summarises the current research addressing the influence of physical activity and exercise in mitigating the risks of obesity and diseases such as type-II diabetes and CVD, through its action on the low-grade inflammatory state. Most research on this topic hypothesised that the association between physical activity and inflammatory markers is independent of fatness, but very few studies have proven this. Given that physical activity and obesity are often inversely related, it is not clear as to whether the anti-inflammatory health benefits of a physically active lifestyle are due to exercise per se or result from favourable changes in the body composition.

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