Facilitating tumor spheroid-based bioassays and in vitro blood vessel modeling via bioinspired self-formation microstructure devices

Lab on a Chip ◽  
2018 ◽  
Vol 18 (16) ◽  
pp. 2453-2465 ◽  
Author(s):  
Ching-Te Kuo ◽  
Siang-Rong Lu ◽  
Wei-Min Chen ◽  
Jong-Yueh Wang ◽  
Si-Chen Lee ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Tumor Spheroid ◽  
Self Formation ◽  
Microstructure Devices

We present a bioinspired self-formation microdevice to boost both tumor spheroid-based bioassays and in vitro modeling of blood vessels.

scholarly journals Ontology groups representing angiogenesis and blood vessels development are highly up-regulated during porcine oviductal epithelial cells long-term real-time proliferation – a primary cell culture approach

2018 ◽  
Vol 6 (4) ◽  
pp. 186-194 ◽  
Author(s):  
Mariusz J. Nawrocki ◽  
Piotr Celichowski ◽  
Maurycy Jankowski ◽  
Wiesława Kranc ◽  
Artur Bryja ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Culture Conditions ◽  
Cellular Interactions ◽  
Biochemical Modification ◽  
Blood Vessel Development ◽  
Development Processes ◽  
Vessel Development

AbstractThe morphological and biochemical modification of oviductal epithelial cells (OECs) belongs to the group of compound processes responsible for proper oocyte transport and successful fertilization. The cellular interactions between cumulus-oocyte complexes (COCs) and oviductal epithelial cells (OECs) are crucial for this unique mechanism. In the present study we have analyzed angiogenesis and blood vessel development processes at transcript levels. By employing microarrays, four ontological groups associated with these mechanisms have been described. Differentially expressed genes belonging to the “angiogenesis”, “blood circulation”, “blood vessel development” and “blood vessel morphogenesis” GO BP terms were investigated as a potential markers for the creation of new blood vessels in cells under in vitro primary culture conditions.

Artery-on-a-chip platform for automated, multimodal assessment of cerebral blood vessel structure and function

Lab on a Chip ◽  
2015 ◽  
Vol 15 (12) ◽  
pp. 2660-2669 ◽  
Author(s):  
Sanjesh Yasotharan ◽  
Sascha Pinto ◽  
John G. Sled ◽  
Steffen-Sebastian Bolz ◽  
Axel Günther
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Structure And Function ◽  
Microfluidic Platform ◽  
Cerebral Blood Vessel ◽  
Multimodal Assessment ◽  
Vessel Structure ◽  
And Function

We present a compact microfluidic platform for the automated, multimodal assessment of intact small blood vesselsin vitro.

scholarly journals Redistribution of surface anionic sites on the luminal front of blood vessel endothelium after interaction with polycationic ligand.

1976 ◽  
Vol 71 (1) ◽  
pp. 232-241 ◽  
Author(s):  
E Skutelsky ◽  
D Danon
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Binding Capacity ◽  
Luminal Surface ◽  
Lateral Migration ◽  
Anionic Sites ◽  
In Vitro Interaction ◽  
Anionic Groups

The ability of anionic groups on the luminal surface of blood vessels to redistribute by lateral migration under the influence of multivalent ligands was analyzed by electron microscopy, using cationized ferritin (CF). In vitro interaction of blood vessel segments with CF results in rapid aggregation of most anionic sites on the luminal fromt of the endothelium, followed by internalization or detachment of the CF patches, leaving most of the luminal surface devoid of anionic sites. Further incubation of such endothelial cells without CF results in regeneration of binding capacity for the polycationic label. Transport of CF, but not of native ferritin, across the endothelium by vesicle transport, followed by exocytosis of the interiorized CF clusters on the tissue front of the endothelium, was also observed. The possibility that such activities in the blood vessels in vivo may be associated with local changes in the normal distribution of the surface anionic sites as well as in accumulation of debris in the subendothelial layers of the vessels is suggested.

Remodeling of the Constitutive Equation While a Blood Vessel Remodels Itself Under Stress

1993 ◽  
Vol 115 (4B) ◽  
pp. 453-459 ◽  
Author(s):  
Y. C. Fung ◽  
S. Q. Liu ◽  
J. B. Zhou
Keyword(s):  
Mechanical Properties ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Vessel Wall ◽  
Elastic Strain Energy ◽  
Growth Stress ◽  
Elastic Behavior ◽  
Stress Strain

Changes in the mechanical properties of a blood vessel when it remodels itself under stress are reviewed. One of the recent findings about blood vessels is the rapidity of tissue remodeling when the blood pressure is changed. When the tissue structure and material composition remodel, the zero-stress state of the vessel changes. The mechanical properties change also in the remodeling process. If the elastic behavior is expressed in terms of a pseudo-elastic strain-energy function, then the constants in the function will change in the course of the remodeling. With all these changes taking place, the scope of constitutive equations broadens: it should now include a mass-and-structure growth-stress relationship as well as a stress-strain-relationship. To obtain the mass-and-structure growth-stress relationship, one must be able to determine the mechanical properties of the different layers of the vessel wall, as well as the chemical composition and morphology. For the blood vessels, new methods of mechanical testing must be introduced. A key thought is to use bending of the blood vessel wall. By bending, different layers of the vessel wall are subjected to different stresses, leading to equations that can be used to solve the inverse problem of determining the stress-strain law from measured stress and strain. In vitro and in vivo experiments and theoretical prospectives are presented.

scholarly journals Role of mosquito saliva in blood vessel location

1984 ◽  
Vol 108 (1) ◽  
pp. 1-7 ◽  
Author(s):  
J. M. Ribeiro ◽  
P. A. Rossignol ◽  
A. Spielman
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Blood Feeding ◽  
Mosquito Saliva ◽  
Feeding Function ◽  
Aggregation Of Platelets ◽  
Biting Behaviour

In order to ascribe a blood feeding function to the saliva of mosquitoes, we determined whether this secretion may limit the initial probing phase of biting behaviour. The probing of hosts was indeed prolonged when the salivary ducts were severed, but this prolongation was absent when mosquitoes were fed on an artificial meal contained beneath a membrane. In vitro, turbidometric assays demonstrated that saliva inhibits the ADP- and collagen-mediated aggregation of platelets. ATP and ADP were hydrolysed by saliva, and this apyrase activity explains, in part, the observed effect upon platelets. We conclude that the saliva of mosquitoes functions by facilitating location of blood vessels.

scholarly journals Lipopolysaccharide-Induced Vascular Inflammation Model on Microfluidic Chip

Micromachines ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 747
Author(s):  
Ungsig Nam ◽  
Seunggyu Kim ◽  
Joonha Park ◽  
Jessie S. Jeon
Keyword(s):  
Cell Adhesion ◽  
Inflammatory Response ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Migration Assay ◽  
Cell Adhesion And Migration ◽  
And Migration

Inflammation is the initiation of defense of our body against harmful stimuli. Lipopolysaccharide (LPS), originating from outer membrane of Gram-negative bacteria, causes inflammation in the animal’s body and can develop several diseases. In order to study the inflammatory response to LPS of blood vessels in vitro, 2D models have been mainly used previously. In this study, a microfluidic device was used to investigate independent inflammatory response of endothelial cells by LPS and interaction of inflamed blood vessel with monocytic THP-1 cells. Firstly, the diffusion of LPS across the collagen gel into blood vessel was simulated using COMSOL. Then, inflammatory response to LPS in engineered blood vessel was confirmed by the expression of Intercellular Adhesion Molecule 1 (ICAM-1) and VE-cadherin of blood vessel, and THP-1 cell adhesion and migration assay. Upregulation of ICAM-1 and downregulation of VE-cadherin in an LPS-treated condition was observed compared to normal condition. In the THP-1 cell adhesion and migration assay, the number of adhered and trans-endothelial migrated THP-1 cells were not different between conditions. However, migration distance of THP-1 was longer in the LPS treatment condition. In conclusion, we recapitulated the inflammatory response of blood vessels and the interaction of THP-1 cells with blood vessels due to the diffusion of LPS.

scholarly journals Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kyuhwan Na ◽  
Youngkyu Cho ◽  
Dong-Hee Choi ◽  
Myung-Jin Park ◽  
Ji-hun Yang ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Gamma Irradiation ◽  
Blood Vessels ◽  
In Vitro Models ◽  
Cell Junctions ◽  
Angiogenic Potential ◽  
Cell Behaviors ◽  
Barrier Formation

AbstractDuring radiotherapy, microenvironments neighboring the tumor are also exposed to gamma irradiation; this results in unexpected side effects. Blood vessels can serve as microenvironments for tumors and they play an important role in providing nutrients to tumors. This is mostly related to tumor progression, metastasis, and relapse after therapy. Many studies have been performed to obtain a better understanding of tumor vasculature after radiotherapy with in vitro models. However, compared to 3-D models, 2-D in vitro endothelial monolayers cannot physiologically reflect in vivo blood vessels. We previously remodeled the extracellular matrix (ECM) hydrogel that enhanced the tight barrier formation of 3-D blood vessels and the vascular endothelial growth factor (VEGF) gradient induced angiogenesis in a microfluidic device. In this study, the blood vessel model is further introduced to understand how gamma irradiation affects the endothelial monolayer. After the gamma irradiation exposure, we observed a collapsed endothelial barrier and a reduced angiogenic potential. Changes in the cell behaviors of the tip and stalk cells were also detected in the angiogenesis model after irradiation, which is difficult to observe in 2-D monolayer models. Therefore, the 3-D in vitro blood vessel model can be used to understand radiation-induced endothelial injuries.

scholarly journals 3D Bioprinted Vascularized Tumour for Drug Testing

2020 ◽  
Vol 21 (8) ◽  
pp. 2993 ◽  
Author(s):  
Seokgyu Han ◽  
Sein Kim ◽  
Zhenzhong Chen ◽  
Hwa Kyoung Shin ◽  
Seo-Yeon Lee ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Drug Testing ◽  
Tumour Size ◽  
Combined Treatment ◽  
Cancer Drug ◽  
Angiogenic Inhibitor ◽  
Anti Cancer

An in vitro screening system for anti-cancer drugs cannot exactly reflect the efficacy of drugs in vivo, without mimicking the tumour microenvironment (TME), which comprises cancer cells interacting with blood vessels and fibroblasts. Additionally, the tumour size should be controlled to obtain reliable and quantitative drug responses. Herein, we report a bioprinting method for recapitulating the TME with a controllable spheroid size. The TME was constructed by printing a blood vessel layer consisting of fibroblasts and endothelial cells in gelatine, alginate, and fibrinogen, followed by seeding multicellular tumour spheroids (MCTSs) of glioblastoma cells (U87 MG) onto the blood vessel layer. Under MCTSs, sprouts of blood vessels were generated and surrounding MCTSs thereby increasing the spheroid size. The combined treatment involving the anti-cancer drug temozolomide (TMZ) and the angiogenic inhibitor sunitinib was more effective than TMZ alone for MCTSs surrounded by blood vessels, which indicates the feasibility of the TME for in vitro testing of drug efficacy. These results suggest that the bioprinted vascularized tumour is highly useful for understanding tumour biology, as well as for in vitro drug testing.

scholarly journals The blood vessels development, morphogenesis and blood circulation are three ontologic groups highly up-regulated in porcine oocytes before in vitro maturation

2017 ◽  
Vol 5 (2) ◽  
pp. 135-142 ◽  
Author(s):  
Mariusz J. Nawrocki ◽  
Piotr Celichowski ◽  
Joanna Budna ◽  
Artur Bryja ◽  
Wiesława Kranc ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Blood Circulation ◽  
In Vitro Maturation ◽  
Embryo Implantation ◽  
Early Embryo ◽  
Expression Of Genes ◽  
Before And After

AbstractThe mammalian oocytes undergo significant biochemical and structural modifications during maturation both in vitro and in vivo. These changes involve chromatin reorganization and modification within metabolic status of cytoplasmic organelles. After oocytes’ successful maturation the substantially increased storage of RNA was observed. Moreover, the early embryo interaction with maternal endometrial tissue after fertilization is up to now considered as the main marker of proper embryo implantation and early growth. In this study, we first investigated the expression profile of genes involved in blood vessel formation and blood circulation in porcine oocytes before and after in vitro maturation.The cumulus-oocyte complexes were collected from pubertal Landrace gilts and classified as before in vitro maturation (in Vivo) or after in vitro maturation (in Vitro). The RNA was isolated from these two experimental groups and analyzed using Affymetrix microarrays.We found an increased expression of genes involved in ontological groups such as “blood circulation” (TPM1, ECE1, ACTA2, EPHX2, EDNRA, NPR2, MYOF, TACR3, VEGFA, GUCY1B3), “blood vessel development” (ANGPTL4, CYR61, SEMA5A, ID1, RHOB, RTN4, IHH, ANGPT2, EDNRA, TGFBR3, MYO1E, MMP14), and “blood vessels morphogenesis” (ANGPT2, as well as other common transcripts) in in Vivo group as compared to decreased expression of these genes in in Vitro group of oocytes.It has been suggested that investigated genes undergo significant expression before in vitro maturation, when enhanced storage of large amount of RNA takes place. Creating templates for synthesis of proteins is required for formation of fully mature gametes and early embryo growth. Therefore we hypothesized that the processes of vascularization and/or angiogenesis reach a high activity in immature oocytes and are distinct from achievement of maturational stage by oocytes in pigs.

Persistence of Fibrinolytic Activity in Fragments of Human Veins Cultured in Vitro

1970 ◽  
Vol 24 (01/02) ◽  
pp. 043-047 ◽  
Author(s):  
M Pandolfi
Keyword(s):  
Blood Vessels ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Vasa Vasorum ◽  
Adult Human ◽  
Slide Technique

SummaryExplants from 5 adult human veins were cultured in a fibrinolytically inactive medium for 3 weeks and assayed for the presence of plasminogen activator by the fibrin slide technique. The explants from 3 veins showed fibrinolytic activity confined to their vasa vasorum for the whole duration of the culture; no decrease of activity was seen. The finding suggests that small blood vessels are able to synthesize plasminogen activator.

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