A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood

Lab on a Chip ◽  
2018 ◽  
Vol 18 (13) ◽  
pp. 1928-1935 ◽  
Author(s):  
Wenhan Liu ◽  
Jagotamoy Das ◽  
Adam H. Mepham ◽  
Carine R. Nemr ◽  
Edward H. Sargent ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Nucleic Acid ◽  
Disease Diagnosis ◽  
Automated Testing ◽  
Nucleic Acid Detection ◽  
Nucleic Acid Testing ◽  
Testing Device ◽  
Viral Nucleic Acid ◽  
Fully Integrated ◽  
Integrated Devices

Integrated devices for automated nucleic acid testing (NAT) are critical for infectious disease diagnosis to be performed outside of centralized laboratories.

The application of viral nucleic acid detection and lung CT in COVID-19 diagnosis

2020 ◽  
Author(s):  
Rui Hu
Keyword(s):  
Nucleic Acid ◽  
Clinical Symptoms ◽  
Virus Disease ◽  
False Negative ◽  
False Negative Rate ◽  
Diagnostic Methods ◽  
Nucleic Acid Detection ◽  
Viral Nucleic Acid ◽  
Early Medical Intervention ◽  
Lung Ct

The Corona Virus Disease 2019 (COVID-19) has the characteristics of fast propagation speed and strong pathogenicity and has attracted wide attention of people, medical workers, and researchers around the world. Accurate, rapid, and timely screening and diagnosis of COVID-19 is of great significance to control the development of the epidemic situation and save the lives of patients. Currently, the detection of viral nucleic acid and lung CT is the main screening and diagnostic methods of COVID-19. Nucleic acid detection has the advantages of fast, strong specificity and high sensitivity, but there is a certain false-negative rate. CT result of lung examination is visual, but it is not typical due to the uncertain time of clinical symptoms and the early medical intervention. Therefore, the diagnosis of COVID-19 should include a combination of epidemiological history, clinical symptoms, imaging, and laboratory tests.

scholarly journals Magnetic Particles for Advanced Molecular Diagnosis

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2158 ◽  
Author(s):  
Cristina Chircov ◽  
Alexandru Mihai Grumezescu ◽  
Alina Maria Holban
Keyword(s):  
Gene Expression ◽  
Infectious Disease ◽  
Nucleic Acid ◽  
Sample Preparation ◽  
Molecular Diagnosis ◽  
Magnetic Particles ◽  
Early Stage ◽  
Disease Diagnosis ◽  
Laboratory Medicine ◽  
Clinical Reality

Molecular diagnosis is the field that aims to develop nucleic-acid-based analytical methods for biological markers and gene expression assessments by combining laboratory medicine and molecular genetics. As it gradually becomes a clinical reality, molecular diagnosis could benefit from improvements resulting from thorough studies that could enhance the accuracy of these methods. The application of magnetic particles in molecular diagnosis tools has led to tremendous breakthroughs in terms of specificity, sensitivity, and discrimination in bioassays. Therefore, the aim of this review is to highlight the principles involved in the implementation of magnetic particles for sample preparation and targeted analyte isolation, purification, and extraction. Furthermore, the most recent advancements in the area of cancer and infectious disease diagnosis are presented, with an emphasis on screening and early stage detection.

Diagnostic Tools for Coronavirus Disease (COVID-19): Comparing CT and RT-PCR Viral Nucleic Acid Testing

2020 ◽  
Vol 215 (4) ◽  
pp. 834-838 ◽  
Author(s):  
Joseph V. Waller ◽  
Parveer Kaur ◽  
Amy Tucker ◽  
Keldon K. Lin ◽  
Michael J. Diaz ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Diagnostic Tools ◽  
Nucleic Acid Testing ◽  
Rt Pcr ◽  
Viral Nucleic Acid

scholarly journals Gastrointestinal symptoms and fecal nucleic acid testing of children with 2019 coronavirus disease: a systematic review and meta-analysis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ji-gan Wang ◽  
Hai-rong Cui ◽  
Hua-bo Tang ◽  
Xiu-li Deng
Keyword(s):  
Nucleic Acid ◽  
Respiratory Tract ◽  
Case Reports ◽  
Meta Analysis ◽  
Clinical Manifestations ◽  
Gastrointestinal Symptoms ◽  
Nucleic Acid Detection ◽  
Nucleic Acid Testing ◽  
Pooled Prevalence ◽  
Positive Rate

Abstract In order to understand the clinical manifestations and incidence of gastrointestinal symptoms of coronavirus disease (COVID-19) in children and discuss the importance of fecal nucleic acid testing.We retrospectively analyzed studies on gastrointestinal symptoms and fecal nucleic acid detection in pediatric COVID-19 patients from January 1, 2020 to August 10, 2020, including prospective clinical studies and case reports. The results of fecal nucleic acid detection were analyzed systematically. Stata12.0 software was used for meta-analysis.The results showed that the most common gastrointestinal symptoms in children with COVID-19 were vomiting and diarrhea, with a total incidence of 17.7% (95% Cl 13.9–21.5%). However, the prevalence of gastrointestinal symptoms in other countries (21.1%, 95% CI 16.5–25.7%) was higher compared to China (12.9%, 95% CI 8–17.7%). In Wuhan, the pooled prevalence was much higher (41.3%, 95% CI 3.2–79.4%) compared to areas outside Wuhan in China (7.1%, 95% CI 4.0–10.3%). The positive rate of fecal nucleic acid testing in COVID-19 children was relatively high at 85.8% (91/106). Additionally, 71.2% (52/73) were still positive for fecal nucleic acid after respiratory tract specimens turned negative. One and two weeks after the respiratory tract specimens turned nucleic acid-negative, 45.2% (33/73) and 34.2% (25/73) patients, respectively, remained fecal nucleic acid-positive. The longest interval between the respiratory tract specimens turning negative and fecal specimens turning negative exceeded 70 days. Conclusions and relevance: gastrointestinal symptoms in pediatric COVID-19 are relatively common. Attention should be paid to the detection of fecal nucleic acids in children. Fecal nucleic acid-negative status should be considered as one of the desegregation standards.

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