Lateral flow assay with pressure meter readout for rapid point-of-care detection of disease-associated protein

Lab on a Chip ◽  
2018 ◽  
Vol 18 (6) ◽  
pp. 965-970 ◽  
Author(s):  
Bingqian Lin ◽  
Zhichao Guan ◽  
Yanling Song ◽  
Eunyeong Song ◽  
Zifei Lu ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Lateral Flow Assays ◽  
Pressure Meter ◽  
User Friendly ◽  
Point Of Care Detection

Paper-based assays such as lateral flow assays are good candidates for portable diagnostics owing to their user-friendly format and low cost.

scholarly journals Integrating high-performing electrochemical transducers in lateral flow assay

2021 ◽  
Author(s):  
Antonia Perju ◽  
Nongnoot Wongkaew
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Analytical Performance ◽  
Label Free ◽  
High Performing ◽  
Lateral Flow Assays ◽  
Electrochemical Transducers

AbstractLateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform. Commercially, visual and optical detection strategies dominate, but it is especially the research on integrating electrochemical (EC) approaches that may have a chance to significantly improve an LFA’s performance that is needed in order to detect analytes reliably at lower concentrations than currently possible. In fact, EC-LFAs offer advantages in terms of quantitative determination, low-cost, high sensitivity, and even simple, label-free strategies. Here, the various configurations of EC-LFAs published are summarized and critically evaluated. In short, most of them rely on applying conventional transducers, e.g., screen-printed electrode, to ensure reliability of the assay, and additional advances are afforded by the beneficial features of nanomaterials. It is predicted that these will be further implemented in EC-LFAs as high-performance transducers. Considering the low cost of point-of-care devices, it becomes even more important to also identify strategies that efficiently integrate nanomaterials into EC-LFAs in a high-throughput manner while maintaining their favorable analytical performance.

Lateral Flow Assay Based on Paper-Hydrogel Hybrid Material for Sensitive Point-of-Care Detection of Dengue Virus

2016 ◽  
Vol 6 (1) ◽  
pp. 1600920 ◽  
Author(s):  
Jane Ru Choi ◽  
Kar Wey Yong ◽  
Ruihua Tang ◽  
Yan Gong ◽  
Ting Wen ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Hybrid Material ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Point Of Care Detection

Duplex-specific nuclease signal amplification-based fluorescent lateral flow assay for the point-of-care detection of microRNAs

The Analyst ◽  
2021 ◽  
Author(s):  
Hongjuan Wei ◽  
Yongjin Peng ◽  
Zikun Bai ◽  
Zhen Rong ◽  
Shengqi Wang
Keyword(s):  
Signal Amplification ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Amplification Strategy ◽  
Point Of Care Detection

We demonstrate a fluorescent lateral flow assay combined with duplex specific nuclease signal amplification strategy for high-sensitive point-of-care detection of cancer-related miRNA.

scholarly journals Electrospun Polycaprolactone Nanofibers as a Reaction Membrane for Lateral Flow Assay

Polymers ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1387 ◽  
Author(s):  
Chee Yew ◽  
Pedram Azari ◽  
Jane Choi ◽  
Farina Muhamad ◽  
Belinda Pingguan-Murphy
Keyword(s):  
Flow Rate ◽  
Point Of Care ◽  
Low Cost ◽  
Average Pore Size ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Average Pore ◽  
Water Contact ◽  
Naoh Solution ◽  
Lateral Flow Assays

Electrospun polycaprolactone (PCL) nanofibers have emerged as a promising material in diverse biomedical applications due to their various favorable features. However, their application in the field of biosensors such as point-of-care lateral flow assays (LFA) has not been investigated. The present study demonstrates the use of electrospun PCL nanofibers as a reaction membrane for LFA. Electrospun PCL nanofibers were treated with NaOH solution for different concentrations and durations to achieve a desirable flow rate and optimum detection sensitivity in nucleic acid-based LFA. It was observed that the concentration of NaOH does not affect the physical properties of nanofibers, including average fiber diameter, average pore size and porosity. However, interestingly, a significant reduction of the water contact angle was observed due to the generation of hydroxyl and carboxyl groups on the nanofibers, which increased their hydrophilicity. The optimally treated nanofibers were able to detect synthetic Zika viral DNA (as a model analyte) sensitively with a detection limit of 0.5 nM. Collectively, the benefits such as low-cost of fabrication, ease of modification, porous nanofibrous structures and tunability of flow rate make PCL nanofibers a versatile alternative to nitrocellulose membrane in LFA applications. This material offers tremendous potential for a broad range of point-of-care applications.

scholarly journals Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection

2020 ◽  
Author(s):  
Susanna K. Elledge ◽  
Xin X. Zhou ◽  
James R. Byrnes ◽  
Alexander J. Martinko ◽  
Irene Lui ◽  
...  
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Low Cost ◽  
Lateral Flow ◽  
Antibody Detection ◽  
Lateral Flow Assays ◽  
Quantitative Results ◽  
Development And Validation ◽  
Antibody Tests ◽  
Split Luciferase

Current serology tests for SARS-CoV-2 antibodies mainly take the form of enzyme-linked immunosorbent assays or lateral flow assays, with the former being laborious and the latter being expensive and often lacking sufficient sensitivity and scalability. Here we present the development and validation of a rapid, low-cost solution-based assay to detect antibodies in serum, plasma, whole blood, and saliva, using rationally designed split luciferase antibody biosensors (spLUC). This new assay, which generates quantitative results in as short as 5 minutes, substantially reduces the complexity and improves the scalability of COVID-19 antibody tests for point-of-care and broad population testing.

scholarly journals Zika virus lateral flow assays using reverse transcription-loop-mediated isothermal amplification

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17800-17808
Author(s):  
Gna Ahn ◽  
SeonHyung Lee ◽  
Se Hee Lee ◽  
Yun Hee Baek ◽  
Min-Suk Song ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Zika Virus ◽  
Detection Method ◽  
Point Of Care ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Independent Point ◽  
Loop Mediated Isothermal Amplification ◽  
Lateral Flow Assays ◽  
Point Of Care Detection

Our study suggest that ZIKV RT-LAMP combined with LFA could serve as a rapid, accurate, and independent point-of-care detection method for ZIKV outbreaks.

scholarly journals Reducing Unspecific Protein Adsorption in Microfluidic Papers Using Fiber-Attached Polymer Hydrogels

Sensors ◽  
2021 ◽  
Vol 21 (19) ◽  
pp. 6348
Author(s):  
Alexander Ritter von Stockert ◽  
Anna Luongo ◽  
Markus Langhans ◽  
Thomas Brandstetter ◽  
Jürgen Rühe ◽  
...  
Keyword(s):  
Protein Transport ◽  
Point Of Care ◽  
Low Cost ◽  
Local Heating ◽  
Free Water ◽  
Lateral Flow ◽  
Paper Strip ◽  
Detection Zone ◽  
Free Paper ◽  
Lateral Flow Assays

Microfluidic paper combines pump-free water transport at low cost with a high degree of sustainability, as well as good availability of the paper-forming cellulosic material, thus making it an attractive candidate for point-of-care (POC) analytics and diagnostics. Although a number of interesting demonstrators for such paper devices have been reported to date, a number of challenges still exist, which limit a successful transfer into marketable applications. A strong limitation in this respect is the (unspecific) adsorption of protein analytes to the paper fibers during the lateral flow assay. This interaction may significantly reduce the amount of analyte that reaches the detection zone of the microfluidic paper-based analytical device (µPAD), thereby reducing its overall sensitivity. Here, we introduce a novel approach on reducing the nonspecific adsorption of proteins to lab-made paper sheets for the use in µPADs. To this, cotton linter fibers in lab-formed additive-free paper sheets are modified with a surrounding thin hydrogel layer generated from photo-crosslinked, benzophenone functionalized copolymers based on poly-(oligo-ethylene glycol methacrylate) (POEGMA) and poly-dimethyl acrylamide (PDMAA). This, as we show in tests similar to lateral flow assays, significantly reduces unspecific binding of model proteins. Furthermore, by evaporating the transport fluid during the microfluidic run at the end of the paper strip through local heating, model proteins can almost quantitatively be accumulated in that zone. The possibility of complete, almost quantitative protein transport in a µPAD opens up new opportunities to significantly improve the signal-to-noise (S/N) ratio of paper-based lateral flow assays.

scholarly journals Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method

2021 ◽  
Vol 9 ◽  
Author(s):  
Mohammad Rahbar ◽  
Yuling Wu ◽  
J. Anand Subramony ◽  
Guozhen Liu
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Quantitative Detection ◽  
Amplification Method ◽  
Silver Amplification

Interleukin-6 (IL-6) is a pro/anti-inflammatory cytokine, the quantitative detection of which has been extensively considered for diagnosis of inflammatory associated diseases. However, there has not yet been a reliable, low-cost, and user-friendly platform developed for point-of-care (POC) detection of IL-6, which will eliminate the conventional costly, time-consuming, and complex assays. In this work, we developed a lateral flow assay for colorimetric detection of IL-6, using anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs) as the detection probes. Silver amplification technique was incorporated with the newly developed assay in order to enhance the obtained colorimetric signals, allowing sensitive detection of IL-6 in human serum in the desired physiological ranges (i.e., 5–1000 pg/mL). A limit of detection of 5 pg/mL could be achieved for IL-6 detection in serum with the amplification step which was not achievable in the standard assay. The corresponding specificity and reproducibility tests were all preformed to confirm the reliability of this assay for quantitative measurement of IL-6 in a POC manner.

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