Characterization of transglutaminase from Bacillus subtilis and its cross-linking function with a bovine serum albumin model

2018 ◽  
Vol 9 (11) ◽  
pp. 5560-5568 ◽  
Author(s):  
Yihan Liu ◽  
Lin Huang ◽  
Dong Zheng ◽  
Yu Fu ◽  
Mengying Shan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Functional Properties ◽  
Bovine Serum ◽  
Ph Stability ◽  
Cross Linking

The functional properties of bovine serum albumin were significantly improved by Bacillus subtilis transglutaminase with good thermal and pH stability.

Development and characterization of enzymatic biosensor based on lipase from porcine pancreas for propylparaben detection

Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Leila Snani ◽  
Saida Zougar ◽  
Fatiha Benamia ◽  
Ilhem Ghodbane
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Pancreatic Lipase ◽  
Bovine Serum ◽  
Breast Tumors ◽  
Cross Linking ◽  
Porcine Pancreatic Lipase ◽  
Experimental Conditions ◽  
Content Type

Purpose The purpose of this paper is to study the immobilization of porcine pancreatic lipase (PPL), in an organic matrix by a covalent cross-linking method to sense propylparaben (PP) present in aqueous solution. Design/methodology/approach PPL immobilization was performed by the covalent cross-linking method, using bovine serum albumin (BSA) in the presence of saturated glutaraldehyde vapor (GA). The preparation of the enzymatic membrane involves the incorporation of porcine pancreatic lipase (PPL), bovine serum albumin (BSA) and glycerol into a phosphate buffer solution (PBS). Characterization of this sensor was performed by impedance spectroscopy (EIS) and scanning electron microscope (SEM). The effect of experimental conditions such as PPL activity, potential, scan rate, PP concentration, pH and presence of interfering elements were studied by cyclic voltammetry. Findings Under the optimal experimental conditions, a number of significant factors were optimized. The method exhibited good linearity in the range of 10–14 to 10–9 mol/L with a good correlation coefficient of 0.957, detection limit (LOD) of 3.66 × 10–15 mol/L and high sensitivity of 1.086 mA mol−1L. The authors also obtained a very good coverage rate of the surface equal to 91.44%, and hydrolytic activity of lipase is evaluated to 26.64 mmol min−1. The stability and the interference were also evaluated. The equivalent circuit used to explain the electrochemical behavior of modified electrode is a Randle circuit. Practical implications The main application of biosensors is the detection of biomolecules that are either indicators of a disease. For example, electrochemical biosensing techniques can be used as clinical tools to detect breast tumors, because these compounds (PP) were found in breast tumors. Originality/value The result registered in this paper indicates that the developed sensor is an efficient, fast, simple and inexpensive analytical tool that can be used for the analysis of water containing PP.

Characterization of binding interaction of triclosan and bovine serum albumin

2019 ◽  
Vol 55 (3) ◽  
pp. 318-325
Author(s):  
Xiaofang Wang ◽  
Luyi Zou ◽  
Chenyu Mi ◽  
Hongyan Yu ◽  
Mengxue Dong ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Binding Interaction

Effect of some drugs and additives on the cross-linking of bovine serum albumin by glutaraldehyde

1989 ◽  
Vol 57 (3) ◽  
pp. 205-210 ◽  
Author(s):  
A.M. Saleh ◽  
L.K. El-Khordagui ◽  
D.A. Robb ◽  
Florence A.T.
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cross Linking ◽  
The Cross

Characterization of bovine serum albumin hydrolysates prepared by subcritical water processing

2019 ◽  
Vol 278 ◽  
pp. 203-207 ◽  
Author(s):  
Bo-Bae Koh ◽  
Eun-Jung Lee ◽  
Karna Ramachandraiah ◽  
Geun-Pyo Hong
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Subcritical Water
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