Pectin structure and particle size modify carotenoid bioaccessibility and uptake by Caco-2 cells in citrus juices vs. concentrates

2018 ◽  
Vol 9 (6) ◽  
pp. 3523-3531 ◽  
Author(s):  
Laura Gence ◽  
Adrien Servent ◽  
Patrick Poucheret ◽  
Abel Hiol ◽  
Claudie Dhuique-Mayer
Keyword(s):  
Particle Size ◽  
Intestinal Cells ◽  
Citrus Juices ◽  
Pectin Structure

Pectin changes from a citrus-based product modify bioaccessibility and carotenoid uptake by intestinal cells.

β-Cryptoxanthin fromCitrusjuices: assessment of bioaccessibility using anin vitrodigestion/Caco-2 cell culture model

2007 ◽  
Vol 97 (5) ◽  
pp. 883-890 ◽  
Author(s):  
Claudie Dhuique-Mayer ◽  
Patrick Borel ◽  
Emmanuelle Reboul ◽  
Bertrand Caporiccio ◽  
Pierre Besancon ◽  
...  
Keyword(s):  
Cell Culture ◽  
Intestinal Cells ◽  
Cell Culture Model ◽  
Simulated Digestion ◽  
Culture Model ◽  
Citrus Juices ◽  
Preferential Uptake ◽  
Β Carotene ◽  
Human Intestinal Cells

β-Cryptoxanthin (β-CX), a provitaminic carotenoid of potential interest for health, is found principally inCitrusfruit in both free and esterified forms. Little is known about the intestinal absorption of β-CX especially with regard to the esterified forms. The aim of this study was to evaluate the absorption of free and esterified β-CX using simulated digestion coupled with the Caco-2 model. Bioaccessibility was investigated by measuring the transfer of carotenoids from different citrus juices into micelles using anin vitrodigestion system. Then, carotenoid uptake was evaluated by adding carotenoid-rich micelles (from thein vitrodigestion) or synthetic micelles (made from synthetic lipids and carotenoids purified from citrus juice) to human intestinal cells (Caco-2 TC7 clone). Our results showed that β-cryptoxanthin esters (β-CXE) were partially hydrolysed during thein vitrodigestion. The bioaccessibility of free β-CX measured was significantly higher (40 (sd1·05) %) than that of β-carotene (30 (sd1·9) %) and β-CXE (16 (sd1·5) %). In the same way, the incorporation of free β-CX (27 (sd1·01) %) into synthetic micelles exceeded (P < 0·05) that of β-carotene (10 (sd0·7) %) and β-CXE (8·8 (sd0·4) %). In the case of micelles fromin vitrodigestion, the uptake of β-carotene, free β-CX and β-CXE forms by Caco-2 cells was 14·3 (sd1·8), 3·9 (sd1·3), and 0·7 (sd0·08) % respectively. These results showed a preferential uptake by Caco-2 cells of β-carotene and free β-CX compared with the two esters of β-CX.

Effects of pressure homogenization on particle size and the functional properties of citrus juices

2009 ◽  
Vol 92 (1) ◽  
pp. 18-23 ◽  
Author(s):  
E. Betoret ◽  
N. Betoret ◽  
J.V. Carbonell ◽  
P. Fito
Keyword(s):  
Particle Size ◽  
Functional Properties ◽  
Citrus Juices

Microstructural characterization of laser-melted/roller-quenched dicalcium silicate

1988 ◽  
Vol 46 ◽  
pp. 582-583
Author(s):  
C. J. Chan ◽  
K. R. Venkatachari ◽  
W. M. Kriven ◽  
J. F. Young
Keyword(s):  
Particle Size ◽  
Raw Materials ◽  
Shape Change ◽  
Microstructural Characterization ◽  
Particle Size Effect ◽  
Dicalcium Silicate ◽  
Laser Melting ◽  
Uniform Size ◽  
Cell Shape Change ◽  
Wide Range

Dicalcium silicate (Ca2SiO4) is a major component of Portland cement. It has also been investigated as a potential transformation toughener alternative to zirconia. It has five polymorphs: α, α'H, α'L, β and γ. Of interest is the β-to-γ transformation on cooling at about 490°C. This transformation, accompanied by a 12% volume increase and a 4.6° unit cell shape change, is analogous to the tetragonal-to-monoclinic transformation in zirconia. Due to the processing methods used, previous studies into the particle size effect were limited by a wide range of particle size distribution. In an attempt to obtain a more uniform size, a fast quench rate involving a laser-melting/roller-quenching technique was investigated.The laser-melting/roller-quenching experiment used precompacted bars of stoichiometric γ-Ca2SiO4 powder, which were synthesized from AR grade CaCO3 and SiO2xH2O. The raw materials were mixed by conventional ceramic processing techniques, and sintered at 1450°C. The dusted γ-Ca2SiO4 powder was uniaxially pressed into 0.4 cm x 0.4 cm x 4 cm bars under 34 MPa and cold isostatically pressed under 172 MPa. The γ-Ca2SiO4 bars were melted by a 10 KW-CO2 laser.

Analytical Electron Microscopy study of two vehicle-aged automotive exhaust catalysts having dissimilar activities

1989 ◽  
Vol 47 ◽  
pp. 272-273
Author(s):  
Sooho Kim ◽  
M. J. D’Aniello
Keyword(s):  
Electron Microscopy ◽  
Particle Size ◽  
Noble Metal ◽  
Catalyst Deactivation ◽  
Analytical Electron Microscopy ◽  
Microscopy Study ◽  
Metal Particles ◽  
Automotive Exhaust ◽  
Exhaust Catalysts ◽  
Analytical Electron

Automotive catalysts generally lose-agtivity during vehicle operation due to several well-known deactivation mechanisms. To gain a more fundamental understanding of catalyst deactivation, the microscopic details of fresh and vehicle-aged commercial pelleted automotive exhaust catalysts containing Pt, Pd and Rh were studied by employing Analytical Electron Microscopy (AEM). Two different vehicle-aged samples containing similar poison levels but having different catalytic activities (denoted better and poorer) were selected for this study.The general microstructure of the supports and the noble metal particles of the two catalysts looks similar; the noble metal particles were generally found to be spherical and often faceted. However, the average noble metal particle size on the poorer catalyst (21 nm) was larger than that on the better catalyst (16 nm). These sizes represent a significant increase over that found on the fresh catalyst (8 nm). The activity of these catalysts decreases as the observed particle size increases.

Effect of particle size on erosion measurements and predictions in annular flow for an elbow

Wear ◽  
2020 ◽  
pp. 203579
Author(s):  
G. Haider ◽  
M. Othayq ◽  
J. Zhang ◽  
R.E. Vieira ◽  
S.A. Shirazi
Keyword(s):  
Particle Size ◽  
Annular Flow ◽  
Effect Of Particle Size
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