Probing the role of dispersion energy on structural transformation of double-stranded xylo- and ribo-nucleic acids

2019 ◽  
Vol 21 (7) ◽  
pp. 3842-3848 ◽  
Author(s):  
Anuj Kumar ◽  
G. Naresh Patwari
Keyword(s):  
Nucleic Acids ◽  
Structural Transformation ◽  
Dispersion Energy

The structural transformation of xylo nucleic acids is marginal in comparison to ribo nucleic acids with change in dispersion energy.

scholarly journals OP0045 NLRP12 INVOLVES IN THE LUPUS WITH POSITIVE INTERFERON SIGNATURE

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 23.2-24
Author(s):  
Y. P. Tsao ◽  
F. Y. Tseng ◽  
C. W. Chao ◽  
M. H. Chen ◽  
S. T. Chen
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Animal Models ◽  
Nucleic Acids ◽  
Disease Activity ◽  
Interferon Signature ◽  
Healthy Donors ◽  
Healthy Control ◽  
Lupus Disease Activity

Background:Systemic lupus erythematous (SLE) is a systemic autoimmune disease with diverse etiological factors. It was recognized that interferon (IFN) signature involved in the progress of SLE. NLRP12 (NOD-like receptor family (NLR) pyrin domain containing 12) is a pyrin containing NLR protein that we had linked its new biological function to the cross-regulation of Toll like receptor (TLRs) and Rig-I like receptor (RIG-I) pathways. NLPR12 acts as an innate immune check-point in regulating type I IFNs expression during TLRs and RIG-I activation. The importance of NLRP12 in lupus disease activity remained to be elucidated.Objectives:To clarify the role of NLRP12 in regulating the interferon signature.Methods:Peripheral blood mononuclear cells (PBMCs) were collected from SLE patients and healthy donors for analysis of NLRP12 and IFN-α gene expression by RT-QPCR. PBMCs were applied for Chromatin immuneprecipitation (ChIP) assay and electrical mobility shift assay (EMSA) to determine the putative transcription factor that regulates NLRP12 expression. An involvement of epigenetic regulation of NLRP12 expression in SLE patients was also analyzed. Bone marrow derived dendritic cells (BMDCs) were collected from wild type mouse and Nlrp12 knocked-out mice. Another CD14+ monocytes were isolated from 10 cases of lupus patients and 8 cases of healthy control, following by stimulating different type of nucleic acids, and IFN-α and IL-6 were measured with ELISA assay. CD14+ monocytes in lupus patients were also pre-treated with IFNAR2 antibody for further nucleic acid stimulation. Two mice models were applied for evaluation the role of Nlrp12: intraperitoneal injection of TMPD (2,6,10,14-tetramethylpentadecane, or pristane) in C57BL/6 mice and Faslpr mice. Both models were conducted with and without Nlrp12 knockout.Results:NLRP12 expression was significantly lower in PBMC isolated from SLE patients compared to healthy donors. The inverse correlation was observed in NLRP12 and IFNA gene expression as well as NLRP12 expression and amount of double-stranded DNA autoantibody in SLE patients. NLRP12 expression showed negative correlations with IFN-α treatment, as well as herpes simplex virus-1 (HSV-1) infection. Results from ChIP and EMSA analysis indicated a potential transcription factor 1 (TF-1) regulating NLRP12 promoter activity. TF-1 lead to transcriptional suppression of NLRP12 in SLE PBMC, and it was gradually induced after IFN treatment. Recruitment of TF-1 to NLRP12 promoter in SLE PBMC compared to the healthy PBMC was detected, and increased when treating with IFN. Human CD14+ monocytes collected from lupus and healthy control stimulating with different type of nucleic acids revealing significant increasing level of IFN-α and IL-6 in lupus patients. Among animal models, both pristine induced mice and Faslpr mice revealed increasing autoantibodies production and severity of glomerulonephritis in Nlrp12-/- group in comparison with Nlrp12+/+ ones, indicating the role of NLRP12 in maintaining positive interferon signature as well as disease activity.Conclusion:Expression level of NLRP1.2 has been demonstrated to be a biomarker of disease activity in SLE patients. The NLRP12 was involved in the interferon signature, which was also negatively regulated by TF-1. Both clinical samples and animal models revealed NLRP12 in maintaining the positive interferon signature, indicating the possible role of exacerbating factor for lupus disease activity.Disclosure of Interests:None declared

STUDIES ON THE MECHANISM OF DECIDUALIZATION. V.

1963 ◽  
Vol 43 (3) ◽  
pp. 462-468 ◽  
Author(s):  
M. C. Shelesnyak ◽  
Liliane Tic
Keyword(s):  
Nucleic Acids ◽  
Metabolic Activity ◽  
Vaginal Smear ◽  
Uterine Weight ◽  
Close Relationship

ABSTRACT The uteri of pseudopregnant rats show a peak of metabolic activity on the 4th and 5th day of leucocytic smear. After the administration of 20 mg of pyrathiazine Cl on the 4th day of pseudopregnancy (L4) in order to induce decidualization of the progestational endometrium, the metabolic activity of the uterus becomes intensified. The amount of synthesis, estimated by determinations of uterine weight, and amount of protein and nucleic acids, was considered as being related to an oestrogen surge occurring on the 3rd day. The present work was undertaken to confirm the relation between the oestrogen surge and the metabolic activity found in the uterus thereafter by using an antioestrogenic substance: ethanoxytriphetol (MER-25). The experiments were performed in pseudopregnant as well as in decidualizing animals. MER-25 was injected on the 3rd day of leucocytic vaginal smear of pseudopregnancy. Analyses of the uterine components 24, 48, 72, 96 h after the injection of the antioestrogen showed a definite inhibition of the synthetic processes in the uterus of the otherwise untreated pseudopregnant rat as well as in decidualizing uterus. The results, confirm the role of oestrogen surge in the processes of decidualization and the close relationship between the oestrogen surge, increase of metabolism and decidualization.

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