Time-resolved FTIR study of light-driven sodium pump rhodopsins

2018 ◽  
Vol 20 (26) ◽  
pp. 17694-17704 ◽  
Author(s):  
Hui-Fen Chen ◽  
Keiichi Inoue ◽  
Hikaru Ono ◽  
Rei Abe-Yoshizumi ◽  
Akimori Wada ◽  
...  
Keyword(s):  
Ftir Spectroscopy ◽  
Sodium Pump ◽  
Functional Class ◽  
Sodium Ion ◽  
Ion Pump ◽  
Time Resolved ◽  
Ftir Study ◽  
Scan Time ◽  
Microbial Rhodopsin

Light-driven sodium ion pump rhodopsin (NaR) is a new functional class of microbial rhodopsin. Present step-scan time-resolved FTIR spectroscopy revealed that the K, L and O intermediates of NaRs contain 13-cis retinal with similar distortion.

scholarly journals Pumping mechanism of NM-R3, a light-driven bacterial chloride importer in the rhodopsin family

2020 ◽  
Vol 6 (6) ◽  
pp. eaay2042
Author(s):  
Ji-Hye Yun ◽  
Mio Ohki ◽  
Jae-Hyun Park ◽  
Naito Ishimoto ◽  
Ayana Sato-Tomita ◽  
...  
Keyword(s):  
Chloride Ion ◽  
Laser Pulses ◽  
Chloride Ions ◽  
Sodium Ion ◽  
Ion Pump ◽  
Conduction Pathway ◽  
Microbial Rhodopsin ◽  
Key Residues ◽  
Structural Insights ◽  
Anion Conduction

A newly identified microbial rhodopsin, NM-R3, from the marine flavobacterium Nonlabens marinus, was recently shown to drive chloride ion uptake, extending our understanding of the diversity of mechanisms for biological energy conversion. To clarify the mechanism underlying its function, we characterized the crystal structures of NM-R3 in both the dark state and early intermediate photoexcited states produced by laser pulses of different intensities and temperatures. The displacement of chloride ions at five different locations in the model reflected the detailed anion-conduction pathway, and the activity-related key residues—Cys105, Ser60, Gln224, and Phe90—were identified by mutation assays and spectroscopy. Comparisons with other proteins, including a closely related outward sodium ion pump, revealed key motifs and provided structural insights into light-driven ion transport across membranes by the NQ subfamily of rhodopsins. Unexpectedly, the response of the retinal in NM-R3 to photostimulation appears to be substantially different from that seen in bacteriorhodopsin.

scholarly journals 1P250 Low-temperature FTIR spectroscopy of a light-driven sodium ion pump(18A. Photobiology: Vision & Photoreception,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S147
Author(s):  
Hikaru Ono ◽  
Keiichi Inoue ◽  
Rei Abe-Yoshizumi ◽  
Kwang-Hwan Jung ◽  
Hideki Kandori
Keyword(s):  
Low Temperature ◽  
Ftir Spectroscopy ◽  
Sodium Ion ◽  
Ion Pump

scholarly journals Electrochemical In Situ Step-scan Time-resolved Microscope FTIR Spectroscopy

2002 ◽  
Vol 18 (11) ◽  
pp. 989-993
Author(s):  
Zhou Zhi-You ◽  
◽  
Sun Shi-Gang ◽  
Chen Sheng-Pei ◽  
Si Di ◽  
...  
Keyword(s):  
Ftir Spectroscopy ◽  
Time Resolved ◽  
Scan Time

In situ step-scan time-resolved microscope FTIR spectroscopy working with a thin-layer cell

2005 ◽  
Vol 7 (5) ◽  
pp. 490-495 ◽  
Author(s):  
Zhi-You Zhou ◽  
Shui-Chao Lin ◽  
Sheng-Pei Chen ◽  
Shi-Gang Sun
Keyword(s):  
Thin Layer ◽  
Ftir Spectroscopy ◽  
Time Resolved ◽  
Layer Cell ◽  
Scan Time ◽  
Thin Layer Cell

scholarly journals 2P237 Low-temperature FTIR spectroscopy of the Light-driven sodium ion pump: Krokinobacter eikastus rhodopsin 2(18A. Photobiology:Vision & Photoreception,Poster)

2014 ◽  
Vol 54 (supplement1-2) ◽  
pp. S234
Author(s):  
Faisal Hammad Mekky Koua ◽  
Rei Abe-Yoshizumi ◽  
Hikaru Ono ◽  
Shota Ito ◽  
Yoshitaka Kato ◽  
...  
Keyword(s):  
Low Temperature ◽  
Ftir Spectroscopy ◽  
Sodium Ion ◽  
Ion Pump
Sign in / Sign up

Export Citation Format

Share Document