Photo-induced linkage isomerization in the gas phase probed by tandem ion mobility and laser spectroscopy

2018 ◽  
Vol 20 (17) ◽  
pp. 12223-12228 ◽  
Author(s):  
Chang Min Choi ◽  
Luke MacAleese ◽  
Philippe Dugourd ◽  
Myoung Choul Choi ◽  
Fabien Chirot
Keyword(s):  
Optical Properties ◽  
Gas Phase ◽  
Laser Spectroscopy ◽  
Ion Mobility ◽  
Light Absorption ◽  
Ruthenium Complexes ◽  
Linkage Isomerization

Ruthenium complexes involving sulfoxide ligands can undergo linkage isomerization upon light absorption, accompanied by dramatic changes in their optical properties.

scholarly journals Ultrafast photoisomerisation of an isolated retinoid

2019 ◽  
Vol 21 (20) ◽  
pp. 10567-10579 ◽  
Author(s):  
James N. Bull ◽  
Christopher W. West ◽  
Cate S. Anstöter ◽  
Gabriel da Silva ◽  
Evan J. Bieske ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Excited State ◽  
Gas Phase ◽  
Laser Spectroscopy ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Photoelectron Imaging ◽  
Time Resolved ◽  
Excited State Dynamics

The photoinduced excited state dynamics of gas-phase trans-retinoate (deprotonated trans-retinoic acid, trans-RA−) are studied using tandem ion mobility spectrometry coupled with laser spectroscopy, and frequency-, angle- and time-resolved photoelectron imaging.

scholarly journals State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis

2021 ◽  
Vol 14 (6) ◽  
pp. 498
Author(s):  
Evolène Deslignière ◽  
Anthony Ehkirch ◽  
Bastiaan L. Duivelshof ◽  
Hanna Toftevall ◽  
Jonathan Sjögren ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility ◽  
State Of The Art ◽  
Native Mass Spectrometry ◽  
Site Specific ◽  
Drug Conjugates ◽  
Conjugation Process ◽  
Antibody Drug

Antibody-drug conjugates (ADCs) are biotherapeutics consisting of a tumor-targeting monoclonal antibody (mAb) linked covalently to a cytotoxic drug. Early generation ADCs were predominantly obtained through non-selective conjugation methods based on lysine and cysteine residues, resulting in heterogeneous populations with varying drug-to-antibody ratios (DAR). Site-specific conjugation is one of the current challenges in ADC development, allowing for controlled conjugation and production of homogeneous ADCs. We report here the characterization of a site-specific DAR2 ADC generated with the GlyCLICK three-step process, which involves glycan-based enzymatic remodeling and click chemistry, using state-of-the-art native mass spectrometry (nMS) methods. The conjugation process was monitored with size exclusion chromatography coupled to nMS (SEC-nMS), which offered a straightforward identification and quantification of all reaction products, providing a direct snapshot of the ADC homogeneity. Benefits of SEC-nMS were further demonstrated for forced degradation studies, for which fragments generated upon thermal stress were clearly identified, with no deconjugation of the drug linker observed for the T-GlyGLICK-DM1 ADC. Lastly, innovative ion mobility-based collision-induced unfolding (CIU) approaches were used to assess the gas-phase behavior of compounds along the conjugation process, highlighting an increased resistance of the mAb against gas-phase unfolding upon drug conjugation. Altogether, these state-of-the-art nMS methods represent innovative approaches to investigate drug loading and distribution of last generation ADCs, their evolution during the bioconjugation process and their impact on gas-phase stabilities. We envision nMS and CIU methods to improve the conformational characterization of next generation-empowered mAb-derived products such as engineered nanobodies, bispecific ADCs or immunocytokines.

scholarly journals Cyclic Ion Mobility–Collision Activation Experiments Elucidate Protein Behavior in the Gas Phase

2021 ◽  
Author(s):  
Charles Eldrid ◽  
Aisha Ben-Younis ◽  
Jakub Ujma ◽  
Hannah Britt ◽  
Tristan Cragnolini ◽  
...  
Keyword(s):  
Gas Phase ◽  
Ion Mobility ◽  
Collision Activation

Recent Advances and the Design Criteria of Metal Sulfide Photocathode and Photoanode for Photoelectrocatalysis

2021 ◽  
Author(s):  
Cui Ying Toe ◽  
Shujie Zhou ◽  
Michael Gunawan ◽  
Xinxin Lu ◽  
Yun Hau Ng ◽  
...  
Keyword(s):  
Optical Properties ◽  
Light Absorption ◽  
Rapid Development ◽  
Metal Sulfide ◽  
Metal Sulfides ◽  
Design Criteria ◽  
Structural And Optical Properties ◽  
Recent Advances ◽  
Favorable Light

Metal sulfides have emerged as promising materials for photoelectrochemical (PEC) applications due to their favorable light absorption ability, tunable structural and optical properties. With the rapid development of PEC systems,...

scholarly journals Di-Tyrosine Cross-Link Decreases the Collisional Cross-Section of Aβ Peptide Dimers and Trimers in the Gas Phase: An Ion Mobility Study

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100200 ◽  
Author(s):  
Ewa Sitkiewicz ◽  
Jacek Olędzki ◽  
Jarosław Poznański ◽  
Michał Dadlez
Keyword(s):  
Gas Phase ◽  
Cross Section ◽  
Ion Mobility ◽  
Aβ Peptide ◽  
Cross Link ◽  
Collisional Cross Section ◽  
Mobility Study
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