DNA aptamer assemblies as fibroblast growth factor mimics and their application in stem cell culture

2019 ◽  
Vol 55 (18) ◽  
pp. 2672-2675 ◽  
Author(s):  
Ryosuke Ueki ◽  
Saki Atsuta ◽  
Ayaka Ueki ◽  
Junya Hoshiyama ◽  
Jingyue Li ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Dna Aptamer ◽  
Fibroblast Growth ◽  
Single Stranded Dna ◽  
Stem Cell Culture

A 76-mer single-stranded DNA acts as functional mimic of basic fibroblast growth factor (bFGF), one of the essential factors for stem cell culture.

scholarly journals A Stable Chimeric Fibroblast Growth Factor (FGF) Can Successfully Replace Basic FGF in Human Pluripotent Stem Cell Culture

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0118931 ◽  
Author(s):  
Yasuko Onuma ◽  
Kumiko Higuchi ◽  
Yasuhiko Aiki ◽  
Yujing Shu ◽  
Masahiro Asada ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Pluripotent Stem Cell ◽  
Fibroblast Growth ◽  
Human Pluripotent Stem Cell ◽  
Basic Fgf ◽  
Stem Cell Culture

scholarly journals Half-life modeling of basic fibroblast growth factor released from growth factor-eluting polyelectrolyte multilayers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ivan Ding ◽  
Amy M. Peterson
Keyword(s):  
Cell Culture ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Half Life ◽  
Cell Types ◽  
Polyelectrolyte Multilayers ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fibroblast Growth ◽  
Cell Culture Study

AbstractGrowth factor-eluting polymer systems have been widely reported to improve cell and tissue outcomes; however, measurements of actual growth factor concentration in cell culture conditions are limited. The problem is compounded by a lack of knowledge of growth factor half-lives, which impedes efforts to determine real-time growth factor concentrations. In this work, the half-life of basic fibroblast growth factor (FGF2) was determined using enzyme linked immunosorbent assay (ELISA). FGF2 release from polyelectrolyte multilayers (PEMs) was measured and the data was fit to a simple degradation model, allowing for the determination of FGF2 concentrations between 2 and 4 days of culture time. After the first hour, the FGF2 concentration for PEMs assembled at pH = 4 ranged from 2.67 ng/mL to 5.76 ng/mL, while for PEMs assembled at pH = 5, the concentration ranged from 0.62 ng/mL to 2.12 ng/mL. CRL-2352 fibroblasts were cultured on PEMs assembled at pH = 4 and pH = 5. After 2 days, the FGF2-eluting PEM conditions showed improved cell count and spreading. After 4 days, only the pH = 4 assembly condition had higher cells counts, while the PEM assembled at pH = 5 and PEM with no FGF2 showed increased spreading. Overall, the half-life model and cell culture study provide optimal concentration ranges for fibroblast proliferation and a framework for understanding how temporal FGF2 concentration may affect other cell types.

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