Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe

2018 ◽  
Vol 54 (89) ◽  
pp. 12630-12633 ◽  
Author(s):  
Peng Sun ◽  
Qianwen Wang ◽  
Bin Yuan ◽  
Qinjun Zhu ◽  
Bin Jiang ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Isotopic Abundance ◽  
Natural Isotopic Abundance ◽  
Alkaline Transition

A solution 1H–14N HSQC experiment has been employed to monitor the alkaline transition of yeast iso-1 cytochrome c.

scholarly journals NMR Reveals the Conformational Changes of Cytochrome C upon Interaction with Cardiolipin

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1031
Author(s):  
Jianhua Zhan ◽  
Guangqing Zhang ◽  
Xin Chai ◽  
Qinjun Zhu ◽  
Peng Sun ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Conformational Changes ◽  
Underlying Mechanism ◽  
Structural Transformations ◽  
Protein Labeling ◽  
Isotopic Abundance ◽  
Natural Isotopic Abundance ◽  
Cyt C ◽  
Activity State ◽  
Partially Unfolded

Conformational change of cytochrome c (cyt c) caused by interaction with cardiolipin (CL) is an important step during apoptosis, but the underlying mechanism is controversial. To comprehensively clarify the structural transformations of cyt c upon interaction with CL and avoid the unpredictable alias that might come from protein labeling or mutations, the conformation of purified yeast iso–1 cyt c with natural isotopic abundance in different contents of CL was measured by using NMR spectroscopy, in which the trimethylated group of the protein was used as a natural probe. The data demonstrate that cyt c has two partially unfolded conformations when interacted with CL: one with Fe–His33 coordination and the other with a penta–coordination heme. The Fe–His33 coordination conformation can be converted into a penta–coordination heme conformation in high content of CL. The structure of cyt c becomes partially unfolded with more exposed heme upon interaction with CL, suggesting that cyt c prefers a high peroxidase activity state in the mitochondria, which, in turn, makes CL easy to be oxidized, and causes the release of cyt c into the cytoplasm as a trigger in apoptosis.

Sequential assignment of NMR spectra of peptides at natural isotopic abundance with Zero and Ultra-Low Field-TOCSY

2021 ◽  
Author(s):  
Alexey S. Kiryutin ◽  
Ivan V. Zhukov ◽  
Fabien Ferrage ◽  
G Bodenhausen ◽  
Alexandra V. Yurkovskaya ◽  
...  
Keyword(s):  
High Field ◽  
Nmr Spectra ◽  
Correlation Spectroscopy ◽  
Sequential Assignment ◽  
Isotopic Abundance ◽  
Natural Isotopic Abundance ◽  
Low Field ◽  
Total Correlation ◽  
High Resolution Nmr ◽  
Novel Method

A novel method dubbed ZULF-TOCSY results from the combination of Zero and Ultra-Low Field (ZULF) with high-field, high-resolution NMR, leading to a generalization of the concept of total correlation spectroscopy...

3D H2BC: A novel experiment for small-molecule and biomolecular NMR at natural isotopic abundance

2009 ◽  
Vol 200 (2) ◽  
pp. 340-343 ◽  
Author(s):  
Sebastian Meier ◽  
Andrew J. Benie ◽  
Jens Ø. Duus ◽  
Ole W. Sørensen
Keyword(s):  
Small Molecule ◽  
Isotopic Abundance ◽  
Biomolecular Nmr ◽  
Natural Isotopic Abundance

Solid-state NMR at natural isotopic abundance for the determination of conformational polymorphism – the case of designed β-turn peptides containing di-prolines

2017 ◽  
Vol 53 (7) ◽  
pp. 1317-1320 ◽  
Author(s):  
Jayasubba Reddy Yarava ◽  
Rajesh Sonti ◽  
K. Kantharaju ◽  
S. Raghothama ◽  
K. V. Ramanathan
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Solution Nmr ◽  
Isotopic Abundance ◽  
Conformational Polymorphism ◽  
Natural Isotopic Abundance

Like nOe in solution NMR, through-space proton correlations have been exploited in the solid state to obtain the cis–trans conformational polymorphism.

scholarly journals The alkaline transition of cytochrome c revisited: Effects of electrostatic interactions and tyrosine nitration on the reaction dynamics

2019 ◽  
Vol 665 ◽  
pp. 96-106 ◽  
Author(s):  
Santiago Oviedo-Rouco ◽  
María A. Castro ◽  
Damián Alvarez-Paggi ◽  
Cecilia Spedalieri ◽  
Verónica Tortora ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Electrostatic Interactions ◽  
Reaction Dynamics ◽  
Tyrosine Nitration ◽  
Alkaline Transition
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