A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases

2018 ◽  
Vol 54 (95) ◽  
pp. 13411-13414 ◽  
Author(s):  
S. Leichnitz ◽  
J. Heinrich ◽  
N. Kulak
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radicals ◽  
Dna Cleavage ◽  
Fluorescence Assay

ROS quench assays for metal-based DNA cleavage show low selectivity and reliability – a fluorogenic assay was thus developed to reliably, selectively and sensitively detect H2O2 and HO˙.

DNA cleavage by hydroxyl radicals generated in a vanadyl ion-hydrogen peroxide system

1992 ◽  
Vol 189 (2) ◽  
pp. 1090-1095 ◽  
Author(s):  
Hiromu Sakurai ◽  
Masami Nakai ◽  
Takao Miki ◽  
Koichiro Tsuchiya ◽  
Jitsuya Takada ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radicals ◽  
Dna Cleavage ◽  
Vanadyl Ion

Removal of Proteinaceous Soils Using Hydroxyl Radicals Generated by the Electrolysis of Hydrogen Peroxide

2002 ◽  
Vol 250 (2) ◽  
pp. 409-414 ◽  
Author(s):  
Koreyoshi Imamura ◽  
Yoichiro Tada ◽  
Hirohumi Tanaka ◽  
Takaharu Sakiyama ◽  
Kazuhiro Nakanishi
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radicals

scholarly journals Killing of Bacillus Spores by Aqueous Dissolved Oxygen, Ascorbic Acid, and Copper Ions

2003 ◽  
Vol 69 (4) ◽  
pp. 2245-2252 ◽  
Author(s):  
J. B. Cross ◽  
R. P. Currier ◽  
D. J. Torraco ◽  
L. A. Vanderberg ◽  
G. L. Wagner ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Dissolved Oxygen ◽  
Hydroxyl Radicals ◽  
Fenton Reaction ◽  
Copper Ions ◽  
Transition Metal Ions ◽  
Fenton Reagent ◽  
Bacillus Spores ◽  
Modified Fenton

ABSTRACT An approach to decontamination of biological endospores is discussed. Specifically, the performance of an aqueous modified Fenton reagent is examined. A modified Fenton reagent formulation of cupric chloride, ascorbic acid, and sodium chloride is shown to be an effective sporicide under aerobic conditions. The traditional Fenton reaction involves the conversion of hydrogen peroxide to hydroxyl radical by aqueous ionic catalysts such as the transition metal ions. Our modified Fenton reaction involves the conversion of aqueous dissolved oxygen to hydrogen peroxide by an ionic catalyst (Cu2+) and then subsequent conversion to hydroxyl radicals. Results are given for the modified Fenton reagent deactivating spores of Bacillus globigii. A biocidal mechanism is proposed that is consistent with our experimental results and independently derived information found in the literature. This mechanism requires diffusion of relatively benign species into the interior of the spore, where dissolved O2 is then converted through a series of reactions which ultimately produce hydroxyl radicals that perform the killing action.

scholarly journals Photochemical Degradation of Sulfadiazine

2013 ◽  
Vol 39 (3) ◽  
pp. 79-91 ◽  
Author(s):  
Natalia Lemańska-Malinowska ◽  
Ewa Felis ◽  
Joanna Surmacz-Górska
Keyword(s):  
Hydrogen Peroxide ◽  
Uv Radiation ◽  
Hydroxyl Radicals ◽  
Rate Constant ◽  
Photochemical Degradation ◽  
Photochemical Processes

Abstract The photochemical degradation of the sulfadiazine (SDZ) was studied. The photochemical processes used in degradation of SDZ were UV and UV/H2O2. In the experiments hydrogen peroxide was applied at different concentrations: 10 mg/dm3 (2.94*10-4 M), 100 mg/dm3 (2.94*10-3 M), 1 g/dm3 (2.94*10-2 M) and 10 g/dm3 (2.94*10-1 M). The concentrations of SDZ during the experiment were controlled by means of HPLC. The best results of sulfadiazine degradation, the 100% removal of the compound, were achieved by photolysis using UV radiation in the presence of 100 mg H2O2/dm3 (2.94*10-3 M). The determined rate constant of sulfadiazine reaction with hydroxyl radicals kOH was equal 1.98*109 M-1s-1.

Bactericidal effect of hydroxyl radicals generated by the sonolysis and photolysis of hydrogen peroxide for endodontic applications

2017 ◽  
Vol 103 ◽  
pp. 65-70 ◽  
Author(s):  
Haruna Ibi ◽  
Makoto Hayashi ◽  
Fumihiko Yoshino ◽  
Muneaki Tamura ◽  
Ayaka Yoshida ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radicals ◽  
Bactericidal Effect

Comparative toxicity of hydrogen peroxide, hydroxyl radicals, and superoxide anion to Escherichia coli

2003 ◽  
Vol 7 (4) ◽  
pp. 961-968 ◽  
Author(s):  
Richard J. Watts ◽  
Diana Washington ◽  
Jimmy Howsawkeng ◽  
Frank J. Loge ◽  
Amy L. Teel
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Hydroxyl Radicals ◽  
Superoxide Anion ◽  
Comparative Toxicity
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