An iridium complex-based probe for photoluminescence lifetime imaging of human carboxylesterase 2 in living cells

2018 ◽  
Vol 54 (65) ◽  
pp. 9027-9030 ◽  
Author(s):  
Zihe Yan ◽  
Jinyu Wang ◽  
Yanxin Zhang ◽  
Sichun Zhang ◽  
Juan Qiao ◽  
...  
Keyword(s):  
Living Cells ◽  
Iridium Complex ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime ◽  
First Time

Human carboxylesterase 2 is detected and imaged in living cells using a photoluminescence lifetime probe for the first time.

Photoluminescence Lifetime Imaging of Synthesized Proteins in Living Cells Using an Iridium-Alkyne Probe

2017 ◽  
Vol 56 (47) ◽  
pp. 14928-14932 ◽  
Author(s):  
Jinyu Wang ◽  
Jie Xue ◽  
Zihe Yan ◽  
Sichun Zhang ◽  
Juan Qiao ◽  
...  
Keyword(s):  
Living Cells ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

Photoluminescence Lifetime Imaging of Synthesized Proteins in Living Cells Using an Iridium-Alkyne Probe

2017 ◽  
Vol 129 (47) ◽  
pp. 15124-15128 ◽  
Author(s):  
Jinyu Wang ◽  
Jie Xue ◽  
Zihe Yan ◽  
Sichun Zhang ◽  
Juan Qiao ◽  
...  
Keyword(s):  
Living Cells ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

Dynamic Monitoring of Phase-Separated Biomolecular Condensates by Photoluminescence Lifetime Imaging

2021 ◽  
Vol 93 (5) ◽  
pp. 2988-2995
Author(s):  
Zihe Yan ◽  
Jianfeng Xue ◽  
Min Zhou ◽  
Jinyu Wang ◽  
Yanxin Zhang ◽  
...  
Keyword(s):  
Dynamic Monitoring ◽  
Lifetime Imaging ◽  
Photoluminescence Lifetime

scholarly journals Assessment of transferrin recycling by Triplet Lifetime Imaging in living cells

2012 ◽  
Vol 3 (10) ◽  
pp. 2526 ◽  
Author(s):  
Matthias Geissbuehler ◽  
Zuzana Kadlecova ◽  
Harm-Anton Klok ◽  
Theo Lasser
Keyword(s):  
Living Cells ◽  
Lifetime Imaging ◽  
Triplet Lifetime

scholarly journals Dynamic nanoscale morphology of the ER surveyed by STED microscopy

2018 ◽  
Vol 218 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Lena K. Schroeder ◽  
Andrew E.S. Barentine ◽  
Holly Merta ◽  
Sarah Schweighofer ◽  
Yongdeng Zhang ◽  
...  
Keyword(s):  
Living Cells ◽  
Stimulated Emission ◽  
Membrane Structures ◽  
Spatiotemporal Resolution ◽  
Sted Microscopy ◽  
Superresolution Microscopy ◽  
Structure And Dynamics ◽  
First Time ◽  
Nanoscale Dynamics ◽  
Conventional Light Microscopy

The endoplasmic reticulum (ER) is composed of interconnected membrane sheets and tubules. Superresolution microscopy recently revealed densely packed, rapidly moving ER tubules mistaken for sheets by conventional light microscopy, highlighting the importance of revisiting classical views of ER structure with high spatiotemporal resolution in living cells. In this study, we use live-cell stimulated emission depletion (STED) microscopy to survey the architecture of the ER at 50-nm resolution. We determine the nanoscale dimensions of ER tubules and sheets for the first time in living cells. We demonstrate that ER sheets contain highly dynamic, subdiffraction-sized holes, which we call nanoholes, that coexist with uniform sheet regions. Reticulon family members localize to curved edges of holes within sheets and are required for their formation. The luminal tether Climp63 and microtubule cytoskeleton modulate their nanoscale dynamics and organization. Thus, by providing the first quantitative analysis of ER membrane structure and dynamics at the nanoscale, our work reveals that the ER in living cells is not limited to uniform sheets and tubules; instead, we suggest the ER contains a continuum of membrane structures that includes dynamic nanoholes in sheets as well as clustered tubules.

scholarly journals Coarse-grained modeling of mitochondrial metabolism enables subcellular flux inference from fluorescence lifetime imaging microscopy

2020 ◽  
Author(s):  
Xingbo Yang ◽  
Daniel J. Needleman
Keyword(s):  
Fluorescence Lifetime ◽  
Metabolic Flux ◽  
Living Cells ◽  
Coarse Grained ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Metabolic Fluxes ◽  
Subcellular Resolution

AbstractMitochondria are central to metabolism and their dysfunctions are associated with many diseases1–9. Metabolic flux, the rate of turnover of molecules through a metabolic pathway, is one of the most important quantities in metabolism, but it remains a challenge to measure spatiotemporal variations in mitochondrial metabolic fluxes in living cells. Fluorescence lifetime imaging microscopy (FLIM) of NADH is a label-free technique that is widely used to characterize the metabolic state of mitochondria in vivo10–18. However, the utility of this technique has been limited by the inability to relate FLIM measurement to the underlying metabolic activities in mitochondria. Here we show that, if properly interpreted, FLIM of NADH can be used to quantitatively measure the flux through a major mitochondrial metabolic pathway, the electron transport chain (ETC), in vivo with subcellular resolution. This result is based on the use of a coarse-grained NADH redox model, which we test in mouse oocytes subject to a wide variety of perturbations by comparing predicted fluxes to direct biochemical measurements and by self-consistency criterion. Using this method, we discovered a subcellular spatial gradient of mitochondrial metabolic flux in mouse oocytes. We showed that this subcellular variation in mitochondrial flux correlates with a corresponding subcellular variation in mitochondrial membrane potential. The developed model, and the resulting procedure for analyzing FLIM of NADH, are valid under nearly all circumstances of biological interest. Thus, this approach is a general procedure to measure metabolic fluxes dynamically in living cells, with subcellular resolution.

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