scholarly journals Super-resolution force spectroscopy reveals ribosomal motion at sub-nucleotide steps

2018 ◽  
Vol 54 (46) ◽  
pp. 5883-5886 ◽  
Author(s):  
Haina Jia ◽  
Yuhong Wang ◽  
Shoujun Xu
Keyword(s):  
Super Resolution ◽  
Force Spectroscopy ◽  
Molecular Force ◽  
Ultrasound Radiation ◽  
Force Resolution

Combination of ultrasound radiation and automated atomic magnetometer provides ultrahigh molecular force resolution that can resolve sub-nucleotide ribosomal motion.

scholarly journals Progress toward the application of molecular force spectroscopy to DNA sequencing

2012 ◽  
Vol 33 (23) ◽  
pp. 3497-3505 ◽  
Author(s):  
Peng Cheng ◽  
Piercen M. Oliver ◽  
Michael J. Barrett ◽  
Dmitri Vezenov
Keyword(s):  
Dna Sequencing ◽  
Force Spectroscopy ◽  
Molecular Force

scholarly journals Probing Binding Pocket of Serotonin Transporter by Single Molecular Force Spectroscopy on Living Cells

2011 ◽  
Vol 287 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Linda Wildling ◽  
Christian Rankl ◽  
Thomas Haselgrübler ◽  
Hermann J. Gruber ◽  
Marion Holy ◽  
...  
Keyword(s):  
Serotonin Transporter ◽  
Force Spectroscopy ◽  
Binding Pocket ◽  
Living Cells ◽  
Molecular Force

scholarly journals Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Aaron Blanchard ◽  
J. Dale Combs ◽  
Joshua M. Brockman ◽  
Anna V. Kellner ◽  
Roxanne Glazier ◽  
...  
Keyword(s):  
T Cell Receptor ◽  
Traction Force ◽  
Super Resolution ◽  
Cell Receptor ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Surface Receptors ◽  
Force Microscopy ◽  
Cellular Processes ◽  
Molecular Force

AbstractMany cellular processes, including cell division, development, and cell migration require spatially and temporally coordinated forces transduced by cell-surface receptors. Nucleic acid-based molecular tension probes allow one to visualize the piconewton (pN) forces applied by these receptors. Building on this technology, we recently developed molecular force microscopy (MFM) which uses fluorescence polarization to map receptor force orientation with diffraction-limited resolution (~250 nm). Here, we show that structured illumination microscopy (SIM), a super-resolution technique, can be used to perform super-resolution MFM. Using SIM-MFM, we generate the highest resolution maps of both the magnitude and orientation of the pN traction forces applied by cells. We apply SIM-MFM to map platelet and fibroblast integrin forces, as well as T cell receptor forces. Using SIM-MFM, we show that platelet traction force alignment occurs on a longer timescale than adhesion. Importantly, SIM-MFM can be implemented on any standard SIM microscope without hardware modifications.

scholarly journals Dielectrophoretic tweezers as a platform for molecular force spectroscopy in a highly parallel format

Lab on a Chip ◽  
2011 ◽  
Vol 11 (24) ◽  
pp. 4248 ◽  
Author(s):  
Peng Cheng ◽  
Michael J. Barrett ◽  
Piercen M. Oliver ◽  
Deniz Cetin ◽  
Dmitri Vezenov
Keyword(s):  
Force Spectroscopy ◽  
Molecular Force
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