scholarly journals Gene assemblyviaone-pot chemical ligation of DNA promoted by DNA nanostructures

2018 ◽  
Vol 54 (36) ◽  
pp. 4529-4532 ◽  
Author(s):  
Ilenia Manuguerra ◽  
Stefano Croce ◽  
Afaf H. El-Sagheer ◽  
Abhichart Krissanaprasit ◽  
Tom Brown ◽  
...  
Keyword(s):  
Dna Nanostructures ◽  
Chemical Ligation ◽  
Dna Nanostructure ◽  
Self Assembled

A gene was obtained from 14 oligonucleotides self-assembled and chemically ligated in a DNA nanostructure.

scholarly journals FOLDNA, a Web Server for Self-Assembled DNA Nanostructure Autoscaffolds and Autostaples

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Chensheng Zhou ◽  
Heng Luo ◽  
Xiaolu Feng ◽  
Xingwang Li ◽  
Jie Zhu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Dna Sequences ◽  
Self Assembly ◽  
Web Server ◽  
Automatic Design ◽  
Dna Nanostructures ◽  
Complementary Dna ◽  
Dna Nanostructure ◽  
Comprehensive Information ◽  
Self Assembled

DNA self-assembly is a nanotechnology that folds DNA into desired shapes. Self-assembled DNA nanostructures, also known as origami, are increasingly valuable in nanomaterial and biosensing applications. Two ways to use DNA nanostructures in medicine are to form nanoarrays, and to work as vehicles in drug delivery. The DNA nanostructures perform well as a biomaterial in these areas because they have spatially addressable and size controllable properties. However, manually designing complementary DNA sequences for self-assembly is a technically demanding and time consuming task, which makes it advantageous for computers to do this job instead. We have developed a web server, FOLDNA, which can automatically design 2D self-assembled DNA nanostructures according to custom pictures and scaffold sequences provided by the users. It is the first web server to provide an entirely automatic design of self-assembled DNA nanostructure, and it takes merely a second to generate comprehensive information for molecular experiments including: scaffold DNA pathways, staple DNA directions, and staple DNA sequences. This program could save as much as several hours in the designing step for each DNA nanostructure. We randomly selected some shapes and corresponding outputs from our server and validated its performance in molecular experiments.

Nanoscale patterning of self-assembled monolayers using DNA nanostructure templates

2016 ◽  
Vol 52 (8) ◽  
pp. 1677-1680 ◽  
Author(s):  
S. P. Surwade ◽  
F. Zhou ◽  
Z. Li ◽  
A. Powell ◽  
C. O'Donnell ◽  
...  
Keyword(s):  
Self Assembled Monolayers ◽  
Dna Nanostructures ◽  
Dna Nanostructure ◽  
Nanoscale Patterning ◽  
Assembled Monolayers ◽  
Self Assembled

We describe a method to pattern arbitrary-shaped silane self-assembled monolayers (SAMs) with nm scale resolution using DNA nanostructures as templates.

A Self-Assembled DNA Nanostructure as FRET Nanoflare for Intracellular ATP Imaging

2021 ◽  
Author(s):  
Cai Shijun ◽  
Jiaoli Wang ◽  
Jing Li ◽  
Bing Zhou ◽  
Chunmei He ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dna Nanostructure ◽  
Intracellular Atp ◽  
Self Assembled

Due to the incorporation of gold nanoparticles (AuNPs), the reported AuNP-based FRET nanoflares still have some problems, such as non-negligible cytotoxicity and time-consuming preparation procudure. In this communication, a novel...

Understanding Self-Assembled Pseudoisocyanine Dye Aggregates in DNA Nanostructures and Their Exciton Relay Transfer Capabilities

2021 ◽  
Author(s):  
Matthew Chiriboga ◽  
Sebastian A. Diaz ◽  
Divita Mathur ◽  
David A. Hastman ◽  
Joseph S. Melinger ◽  
...  
Keyword(s):  
Dna Nanostructures ◽  
Self Assembled

An electrochemical aptasensor based on cocoon-like DNA nanostructure signal amplification for the detection of Escherichia coli O157:H7

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7340-7348
Author(s):  
Huasong Bai ◽  
Shengjun Bu ◽  
Wensen Liu ◽  
Chengyu Wang ◽  
Zhongyi Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Selective Detection ◽  
Escherichia Coli O157 ◽  
Dna Nanostructures ◽  
Electrochemical Aptasensor ◽  
Dna Nanostructure ◽  
Highly Sensitive

We developed an electrochemical aptasensor based on cocoon-like DNA nanostructures as signal tags for highly sensitive and selective detection of Escherichia coli O157:H7.

Self-Assembled DNA Nanostructures-Based Nanocarriers Enabled Functional Nucleic Acids Delivery

2020 ◽  
Vol 3 (5) ◽  
pp. 2779-2795 ◽  
Author(s):  
Jin Huang ◽  
Wenjie Ma ◽  
Huanhuan Sun ◽  
Huizhen Wang ◽  
Xiaoxiao He ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dna Nanostructures ◽  
Self Assembled ◽  
Nucleic Acids Delivery ◽  
Functional Nucleic Acids

scholarly journals DNA nanostructures coordinate gene silencing in mature plants

2019 ◽  
Vol 116 (15) ◽  
pp. 7543-7548 ◽  
Author(s):  
Huan Zhang ◽  
Gozde S. Demirer ◽  
Honglu Zhang ◽  
Tianzheng Ye ◽  
Natalie S. Goh ◽  
...  
Keyword(s):  
Cell Wall ◽  
Gene Silencing ◽  
Plant Cell ◽  
Mammalian Cells ◽  
Plant Cell Wall ◽  
Plant Cells ◽  
Design Parameters ◽  
Dna Nanostructures ◽  
Dna Nanostructure ◽  
The Impact

Delivery of biomolecules to plants relies onAgrobacteriuminfection or biolistic particle delivery, the former of which is amenable only to DNA delivery. The difficulty in delivering functional biomolecules such as RNA to plant cells is due to the plant cell wall, which is absent in mammalian cells and poses the dominant physical barrier to biomolecule delivery in plants. DNA nanostructure-mediated biomolecule delivery is an effective strategy to deliver cargoes across the lipid bilayer of mammalian cells; however, nanoparticle-mediated delivery without external mechanical aid remains unexplored for biomolecule delivery across the cell wall in plants. Herein, we report a systematic assessment of different DNA nanostructures for their ability to internalize into cells of mature plants, deliver siRNAs, and effectively silence a constitutively expressed gene inNicotiana benthamianaleaves. We show that nanostructure internalization into plant cells and corresponding gene silencing efficiency depends on the DNA nanostructure size, shape, compactness, stiffness, and location of the siRNA attachment locus on the nanostructure. We further confirm that the internalization efficiency of DNA nanostructures correlates with their respective gene silencing efficiencies but that the endogenous gene silencing pathway depends on the siRNA attachment locus. Our work establishes the feasibility of biomolecule delivery to plants with DNA nanostructures and both details the design parameters of importance for plant cell internalization and also assesses the impact of DNA nanostructure geometry for gene silencing mechanisms.

scholarly journals Crystallization of a self-assembled three-dimensional DNA nanostructure

2013 ◽  
Vol 69 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Kimberly N. Rendek ◽  
Raimund Fromme ◽  
Ingo Grotjohann ◽  
Petra Fromme
Keyword(s):  
Three Dimensional ◽  
Dna Nanostructure ◽  
Self Assembled
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