Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification

2018 ◽  
Vol 54 (19) ◽  
pp. 2413-2416 ◽  
Author(s):  
Fei Ma ◽  
Wen-jing Liu ◽  
Li Liang ◽  
Bo Tang ◽  
Chun-Yang Zhang
Keyword(s):  
Alkaline Phosphatase ◽  
Signal Amplification ◽  
Fluorescence Method ◽  
Sensitive Detection ◽  
Dual Signal Amplification

We develop a new fluorescence method for the sensitive detection of alkaline phosphatase based on dephosphorylation-initiated transcription reaction-mediated dual signal amplification.

Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

2017 ◽  
Vol 53 (27) ◽  
pp. 3878-3881 ◽  
Author(s):  
Li-Juan Wang ◽  
Zi-Yue Wang ◽  
Qianyi Zhang ◽  
Bo Tang ◽  
Chun-Yang Zhang
Keyword(s):  
Real Time ◽  
Signal Amplification ◽  
Fluorescence Method ◽  
Dna Glycosylase ◽  
Real Time Monitoring ◽  
Thymine Dna Glycosylase ◽  
Dual Signal Amplification

We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase activity through cyclic enzymatic repairing-mediated dual-signal amplification.

scholarly journals Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4364
Author(s):  
Shouping Zhang ◽  
Bin Hu ◽  
Xiaojing Xia ◽  
Yanzhao Xu ◽  
Bolin Hang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Secondary Infection ◽  
Porcine Circovirus Type ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Porcine Circovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Ability ◽  
Strong Binding ◽  
Dual Signal Amplification

The frequent emergence of secondary infection and immunosuppression after porcine circovirus type 2 (PCV2) infection highlights the need to develop sensitive detection methods. A dual-signal amplification enzyme-linked immunosorbent assay (ELISA) based on a microplate coated with gold nanoparticle layers (GNPL) and tyramide signal amplification (TSA) was established. Results confirmed that the microplates coated with GNPL have a strong binding ability to the antibody without affecting the biological activity of the antibody. The microplates coated with GNPL have strong binding ability to the antibody, and the amplification of the tyramide signal is combined to further improve the sensitivity of PCV2. The PCV2 antibody does not crossreact with other viruses, demonstrating that the method has good specificity. A dual-signal amplification strategy is developed using microplates modified with GNPL and TSA to sensitively detect PCV2.

A highly sensitive photoelectrochemical VEGF165 biosensor with a dual signal amplification strategy by using AgVO3 as a photoactive material

2019 ◽  
Vol 55 (56) ◽  
pp. 8076-8078 ◽  
Author(s):  
Huimei Da ◽  
Yaling Liu ◽  
Mengjie Li ◽  
Ruo Yuan ◽  
Hongyan Liu ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Vascular Endothelial ◽  
Background Signal ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification ◽  
Endothelial Growth Factor

We developed a novel “signal-on” photoelectrochemical (PEC) aptasensor with a near-zero background signal by using AgVO3 as a single photoactive material for the sensitive detection of vascular endothelial growth factor (VEGF165).

Construction of aptasensors for sensitive detection of 8-OH-dG based on a diffusion mediated electrochemiluminescence quenching effect

2020 ◽  
Vol 56 (75) ◽  
pp. 11074-11077
Author(s):  
Zhe Feng ◽  
Rui-Juan Zhao ◽  
Ze-Hua Lu ◽  
Li-ping Jia ◽  
Rong-Na Ma ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Dna Immobilization ◽  
Quenching Effect ◽  
Dual Signal Amplification

A DNA immobilization-free electrochemiluminescence aptasensor was developed for the detection of 8-hydroxy-2′-deoxygunosine based on the diffusion mediated electrochemiluminescence quenching effect and dual signal amplification strategies.

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