scholarly journals Identifying key barriers in cationic polymer gene delivery to human T cells

2019 ◽  
Vol 7 (3) ◽  
pp. 789-797 ◽  
Author(s):  
Brynn R. Olden ◽  
Emmeline Cheng ◽  
Yilong Cheng ◽  
Suzie H. Pun
Keyword(s):  
T Cells ◽  
Gene Delivery ◽  
Intracellular Ph ◽  
Cationic Polymer ◽  
Cationic Polymers ◽  
Human T Cells

Decreased uptake of polyplexes and an increased intracellular pH contribute to poor gene delivery to human T cellsviacationic polymers.

scholarly journals Cationic polymers for non-viral gene delivery to human T cells

2018 ◽  
Vol 282 ◽  
pp. 140-147 ◽  
Author(s):  
Brynn R. Olden ◽  
Yilong Cheng ◽  
Jonathan L. Yu ◽  
Suzie H. Pun
Keyword(s):  
T Cells ◽  
Gene Delivery ◽  
Viral Gene ◽  
Cationic Polymers ◽  
Human T Cells ◽  
Viral Gene Delivery

Cationic polymer-derived carbon dots for enhanced gene delivery and cell imaging

2019 ◽  
Vol 7 (5) ◽  
pp. 1940-1948 ◽  
Author(s):  
Xi He ◽  
Ping Chen ◽  
Ji Zhang ◽  
Tian-Ying Luo ◽  
Hai-Jiao Wang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Carbon Dots ◽  
Ring Opening ◽  
Cell Imaging ◽  
High Efficiency ◽  
Ring Opening Polymerization ◽  
Cationic Polymer ◽  
Cationic Polymers ◽  
Promising Strategy ◽  
Gene Vectors

The transformation of cationic polymers derived from ring-opening polymerization to carbon dots was proved as a promising strategy for developing gene vectors with high efficiency and cell-imaging ability.

scholarly journals High-efficiency Non-viral Transfection of Human Primary T Lymphocytes

2020 ◽  
Author(s):  
Hossein Khanahmad ◽  
Ilnaz Rahimmanesh ◽  
Mehdi Totonchi
Keyword(s):  
T Cells ◽  
Gene Delivery ◽  
High Efficiency ◽  
Positive Charge ◽  
Genetic Manipulation ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Delivery Method ◽  
Human T Cells ◽  
Viral Gene Delivery

The development and optimization of an effective non-viral gene delivery method for genetic manipulation of primary human T cells is a major challenge in clinical immunotherapy researches. According to the low transfection efficiency of conventional methods in human primary T cells, there is an effort in order to increase the transfection rate in these cells. Protamine is an FDA-approved compound with a documented safety profile that enhances DNA condensation for gene delivery. In this study, the effect of protamine on the transfection efficiency of standard transfection reagents, TurboFect, and Lipofectamine 2000 was evaluated in order to transfect primary human T cells. Results demonstrated that protamine condenses DNA and increases the positive charge of DNA/Cargo complex efficiently without any cytotoxic effect on the primary human T cells. The results also revealed that the DNA/Protamine/Cargo complexes effectively transfect human primary T cells.

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