Differentiating human pluripotent stem cells into vascular smooth muscle cells in three dimensional thermoreversible hydrogels

2019 ◽  
Vol 7 (1) ◽  
pp. 347-361 ◽  
Author(s):  
Haishuang Lin ◽  
Qian Du ◽  
Qiang Li ◽  
Ou Wang ◽  
Zhanqi Wang ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Human Pluripotent Stem Cell ◽  
Scalable Production

3D thermoreversible PNIPAAm-PEG hydrogels are used for scalable production of human pluripotent stem cell-derived vascular smooth muscle cells.

scholarly journals Induced pluripotent stem cell-derived vascular smooth muscle cells: methods and application

2015 ◽  
Vol 465 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Biraja C. Dash ◽  
Zhengxin Jiang ◽  
Carol Suh ◽  
Yibing Qyang
Keyword(s):  
Smooth Muscle ◽  
Stem Cell ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Muscle Cells ◽  
Regenerative Therapy ◽  
Induced Pluripotent

Vascular smooth muscle cells (VSMCs) play a major role in the pathophysiology of cardiovascular diseases. The advent of induced pluripotent stem cell (iPSC) technology and the capability of differentiating into virtually every cell type in the human body make this field a ray of hope for vascular regenerative therapy and understanding of the disease mechanism. In the present review, we first discuss the recent iPSC technology and vascular smooth muscle development from an embryo and then examine different methodologies to derive VSMCs from iPSCs, and their applications in regenerative therapy and disease modelling.

Morphology of vascular smooth muscle cells and pericytes as revealed by transmission and scanning EM

1978 ◽  
Vol 36 (2) ◽  
pp. 464-465
Author(s):  
Y. Uehara ◽  
T. Komuro ◽  
J. Desaki
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cell ◽  
Vascular Function ◽  
Three Dimensional ◽  
Muscle Cells ◽  
Structural Level ◽  
Scanning Em

Although subcellular organization of vascular smooth muscle cells and pericytes have been described by a number of authors, the precise overall structure of these cells has not been revealed at the fine structural level.In order to extend our knowledge in understanding of vascular function, the present paper deals the three dimensional organization of these cells as studied with a transmission EM using serial sections and with a scanning EM using materials from which connective tissue elements are removed.For the reconstruction study, arterioles ranging in diameter from l0um to 25um are selected from the hamster skeletal muscle and the rat pancreas.Fig. 1. demonstrates an example of the reconstructed model of the arteriole smooth muscle cell. Here the muscle cell takes a spiral form which coils nearly twice around the endothelial column. The shape and disposition of the muscle cells gradually become to be irregular towards the capillary bed.

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