Enhanced osteogenic differentiation and bone regeneration of poly(lactic-co-glycolic acid) by graphene via activation of PI3K/Akt/GSK-3β/β-catenin signal circuit

2018 ◽  
Vol 6 (5) ◽  
pp. 1147-1158 ◽  
Author(s):  
Xiaowei Wu ◽  
Shang Zheng ◽  
Yuanzhou Ye ◽  
Yuchen Wu ◽  
Kaili Lin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Glycolic Acid ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Autologous Bone ◽  
Potential Strategy ◽  
Gsk 3Β ◽  
Reconstruction Of Bone Defects

The reconstruction of bone defects by guiding autologous bone tissue regeneration with graphene-based biomaterials is a potential strategy in the area of bone tissue engineering.

scholarly journals Dual Network Composites of Poly(vinyl alcohol)-Calcium Metaphosphate/Alginate with Osteogenic Ions for Bone Tissue Engineering in Oral and Maxillofacial Surgery

2021 ◽  
Vol 8 (8) ◽  
pp. 107
Author(s):  
Lilis Iskandar ◽  
Lucy DiSilvio ◽  
Jonathan Acheson ◽  
Sanjukta Deb
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Vinyl Alcohol ◽  
Maxillofacial Surgery ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Poly Vinyl Alcohol ◽  
Oral And Maxillofacial Surgery

Despite considerable advances in biomaterials-based bone tissue engineering technologies, autografts remain the gold standard for rehabilitating critical-sized bone defects in the oral and maxillofacial (OMF) region. A majority of advanced synthetic bone substitutes (SBS’s) have not transcended the pre-clinical stage due to inferior clinical performance and translational barriers, which include low scalability, high cost, regulatory restrictions, limited advanced facilities and human resources. The aim of this study is to develop clinically viable alternatives to address the challenges of bone tissue regeneration in the OMF region by developing ‘dual network composites’ (DNC’s) of calcium metaphosphate (CMP)—poly(vinyl alcohol) (PVA)/alginate with osteogenic ions: calcium, zinc and strontium. To fabricate DNC’s, single network composites of PVA/CMP with 10% (w/v) gelatine particles as porogen were developed using two freeze–thawing cycles and subsequently interpenetrated by guluronate-dominant sodium alginate and chelated with calcium, zinc or strontium ions. Physicochemical, compressive, water uptake, thermal, morphological and in vitro biological properties of DNC’s were characterised. The results demonstrated elastic 3D porous scaffolds resembling a ‘spongy bone’ with fluid absorbing capacity, easily sculptable to fit anatomically complex bone defects, biocompatible and osteoconductive in vitro, thus yielding potentially clinically viable for SBS alternatives in OMF surgery.

PCL and PCL/PLA Scaffolds for Bone Tissue Regeneration

2013 ◽  
Vol 683 ◽  
pp. 168-171 ◽  
Author(s):  
Tatiana Patrício ◽  
Antonio Gloria ◽  
Paulo J. Bártolo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Cell Attachment ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Printing System

This paper investigates the use of PCL and PCL/PLA scaffolds, produced using a novel additive biomanufacturing system called BioCell Printing, for bone tissue engineering applications. Results show that the BioCell Printing system produces scaffolds with regular and reproducible architecture, presenting no toxicity and enhancing cell attachment and proliferation. It was also possible to observe that the addition of PLA to PCL scaffolds strongly improves the biomechanical performance of the constructs.

scholarly journals Bone Tissue Regeneration in the Oral and Maxillofacial Region: A Review on the Application of Stem Cells and New Strategies to Improve Vascularization

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Vivian Wu ◽  
Marco N. Helder ◽  
Nathalie Bravenboer ◽  
Christiaan M. ten Bruggenkate ◽  
Jianfeng Jin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Host Tissue ◽  
Bone Tissue Regeneration ◽  
Maxillofacial Region ◽  
Oral And Maxillofacial Region ◽  
New Strategies

Bone tissue engineering techniques are a promising alternative for the use of autologous bone grafts to reconstruct bone defects in the oral and maxillofacial region. However, for successful bone regeneration, adequate vascularization is a prerequisite. This review presents and discusses the application of stem cells and new strategies to improve vascularization, which may lead to feasible clinical applications. Multiple sources of stem cells have been investigated for bone tissue engineering. The stromal vascular fraction (SVF) of human adipose tissue is considered a promising single source for a heterogeneous population of essential cells with, amongst others, osteogenic and angiogenic potential. Enhanced vascularization of tissue-engineered grafts can be achieved by different mechanisms: vascular ingrowth directed from the surrounding host tissue to the implanted graft, vice versa, or concomitantly. Vascular ingrowth into the implanted graft can be enhanced by (i) optimizing the material properties of scaffolds and (ii) their bioactivation by incorporation of growth factors or cell seeding. Vascular ingrowth directed from the implanted graft towards the host tissue can be achieved by incorporating the graft with either (i) preformed microvascular networks or (ii) microvascular fragments (MF). The latter may have stimulating actions on both vascular ingrowth and outgrowth, since they contain angiogenic stem cells like SVF, as well as vascularized matrix fragments. Both adipose tissue-derived SVF and MF are cell sources with clinical feasibility due to their large quantities that can be harvested and applied in a one-step surgical procedure. During the past years, important advancements of stem cell application and vascularization in bone tissue regeneration have been made. The development of engineered in vitro 3D models mimicking the bone defect environment would facilitate new strategies in bone tissue engineering. Successful clinical application requires innovative future investigations enhancing vascularization.

Fibers Obtaining and Characterization Using Poly (Lactic-co-Glycolic Acid) and Poly (Isoprene) Containing Hydroxyapatite and α TCP Calcium Phosphate by Electrospinning Method

2014 ◽  
Vol 631 ◽  
pp. 173-178
Author(s):  
Fernanda Albrecht Vechietti ◽  
D. Marques ◽  
Nathália Oderich Muniz ◽  
Luis Alberto Santos
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Glycolic Acid ◽  
Bone Tissue Regeneration ◽  
Morphological Properties ◽  
Polymeric Fibers ◽  
Natural Bone ◽  
Electrospinning Method ◽  
Biological Apatite ◽  
The One

Natural bone is formed by a complex composite, essentially constituted of biological apatite and fibers of collagen. The combination of materials such as biopolymers and bioceramics may result in an interesting material for application in bone tissue regeneration. This work aims to obtain polymeric fibers containing Poly (Lactic-co-Glycolic Acid) and Poly (Isoprene), supplemented with hydroxyapatite (HA) and α-tricalcium phosphate (TCP). The thermal, mechanical and morphological properties of the fibers were evaluated . Even presenting a larger diameter, fibers with α-TCP presented lower elastic modulus than fibers with HA. Both fibers presented similar thermal behavior, with glass transition temperature in the same range that the one presented by raw PLGA and similar degradation temperatures. Is safe to say that the presence of ceramics in the fibers have a potential for further investigations aiming bone tissue regeneration.

Comparison among Four Kinds of Bone Tissue Engineering Scaffold

2007 ◽  
Vol 330-332 ◽  
pp. 963-966 ◽  
Author(s):  
Lei Liu ◽  
Run Liang Chen ◽  
Yun Feng Lin ◽  
Cai Li ◽  
Wei Dong Tian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Defects ◽  
Marrow Stromal Cells ◽  
Autogenous Bone ◽  
Calvarial Defects ◽  
Reconstruction Of Bone Defects

Bone tissue engineering is a promising way to repair of bone defects. To choose a proper scaffold is still a disputable problem in bone tissue engineering. This study aimed to compare the effects of repairing critical calvarial defects with the compounds of autogenous bone marrow stromal cells (BMSCs) and coral hydroxyapatite(CHA), hydroxyapatite/ tricalcium phosphate (HA/TCP), poly(lactide-co-glycolide) (PLGA) and alginate (AG). The results showed that CHA and AG were satisfactory bone tissues engineering scaffolds among the four kinds of materials. BMSCs/CHA and BMSCs/AG are promising techniques for reconstruction of bone defects.

Biological and mechanical evaluation of poly(lactic-co-glycolic acid)-based composites reinforced with 1D, 2D and 3D carbon biomaterials for bone tissue regeneration

2017 ◽  
Vol 12 (2) ◽  
pp. 025012 ◽  
Author(s):  
Tejinder Kaur ◽  
Senthilguru Kulanthaivel ◽  
Arunachalam Thirugnanam ◽  
Indranil Banerjee ◽  
Krishna Pramanik
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Glycolic Acid ◽  
Bone Tissue Regeneration ◽  
2D And 3D

scholarly journals Carbon Nanostructures in Bone Tissue Engineering

2016 ◽  
Vol 10 (1) ◽  
pp. 877-899 ◽  
Author(s):  
Brian Lee Perkins ◽  
Naghmeh Naderi
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Carbon Nanostructures ◽  
Bone Tissue Regeneration ◽  
Biophysical Properties ◽  
Carbon Nanostructure ◽  
Natural Bone ◽  
Wide Range

Background:Recent advances in developing biocompatible materials for treating bone loss or defects have dramatically changed clinicians’ reconstructive armory. Current clinically available reconstructive options have certain advantages, but also several drawbacks that prevent them from gaining universal acceptance. A wide range of synthetic and natural biomaterials is being used to develop tissue-engineered bone. Many of these materials are currently in the clinical trial stage.Methods:A selective literature review was performed for carbon nanostructure composites in bone tissue engineering.Results:Incorporation of carbon nanostructures significantly improves the mechanical properties of various biomaterials to mimic that of natural bone. Recently, carbon-modified biomaterials for bone tissue engineering have been extensively investigated to potentially revolutionize biomaterials for bone regeneration.Conclusion:This review summarizes the chemical and biophysical properties of carbon nanostructures and discusses their functionality in bone tissue regeneration.

scholarly journals Mesoporous Silica Based Nanostructures for Bone Tissue Regeneration

2021 ◽  
Vol 8 ◽  
Author(s):  
Sougata Ghosh ◽  
Thomas J. Webster
Keyword(s):  
Tissue Engineering ◽  
Mesoporous Silica ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Mesoporous Silica Nanoparticles ◽  
Fluorescein Isothiocyanate ◽  
Bone Tissue Regeneration ◽  
Surface Modified ◽  
Remarkable Progress

Porous nano-scaffolds provide for better opportunities to restore, maintain, and improve functions of damaged tissues and organs by facilitating tissue regeneration. Various nanohybrids composed of mesoporous silica nanoparticles (MSNs) are being widely explored for tissue engineering. Since biological activity is enhanced by several orders of magnitude in multicomponent scaffolds, remarkable progress has been observed in this field, which has aimed to develop the controlled synthesis of multifunctional MSNs with tuneable pore size, efficient delivering capacity of bioactive factors, as well as enhanced biocompatibility and biodegradability. In this review, we aim to provide a broad survey of the synthesis of multifunctional MSN based nanostructures with exotic shapes and sizes. Further, their promise as a novel nanomedicine is also elaborated with respect to their role in bone tissue engineering. Also, recent progress in surface modification and functionalization with various polymers like poly (l-lactic acid)/poly (ε-caprolactone), polylysine-modified polyethylenimine, poly (lactic-co-glycolic acid), and poly (citrate-siloxane) and biological polymers like alginate, chitosan, and gelatine are also covered. Several attempts for conjugating drugs like dexamethasone and β–estradiol, antibiotics like vancomycin and levofloxaci, and imaging agents like fluorescein isothiocyanate and gadolinium, on the surface modified MSNs are also covered. Finally, the scope of developing orthopaedic implants and potential trends in 3D bioprinting applications of MSNs are also discussed. Hence, MSNs based nanomaterials may serve as improved candidate biotemplates or scaffolds for numerous bone tissue engineering, drug delivery and imaging applications deserving our full attention now.

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