Spin probe method of electron paramagnetic resonance spectroscopy – a qualitative test for measuring the evolution of dry eye syndrome under treatment

2019 ◽  
Vol 11 (7) ◽  
pp. 965-972 ◽  
Author(s):  
Mihaela Monica Constantin ◽  
Catalina Gabriela Corbu ◽  
Cristiana Tanase ◽  
Elena Codrici ◽  
Simona Mihai ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Dry Eye ◽  
Spin Probe ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Probe Method ◽  
Dry Eye Syndrome ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Qualitative Test ◽  
Electron Paramagnetic

The spin probe 5-DSA binds to proteins from tears and can be used to monitor changes in tear composition.

scholarly journals Evaluation of Dry Eye Symptomatology at Patients with Keratoconus

2019 ◽  
Vol 70 (1) ◽  
pp. 92-95
Author(s):  
Mihaela Monica Constantin ◽  
Catalina Corbu ◽  
Vasile Potop ◽  
Miruna Burcel ◽  
Sorin Mocanu ◽  
...  
Keyword(s):  
Dry Eye ◽  
Spin Probe ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Control Group ◽  
Resonance Spectroscopy ◽  
Ophthalmological Examination ◽  
Ocular Surface Disease Index ◽  
Paramagnetic Resonance ◽  
Mean Values ◽  
Electron Paramagnetic

The aim of this study was firstly to determine and quantify the dry eye symptomatology of patients with keratoconus, in comparison with those without this condition and secondly to evaluate the suitability of the spin probe method of electron paramagnetic resonance spectroscopy in analysis of tears secretion. As such were considered two groups of patients for evaluation: 36 cases with keratoconus (keratoconus group) and 36 cases without ocular disease (control group). Besides general ophthalmological examination we have conducted Schirmer test I, tears break-up time (BUT) and quantify the answers about dry eye symptomatology to establish ocular surface disease index (OSDI). We have included a prospective correlation of ophthalmologic parameters with parameters of the spin probes added in tear secretions collected from both groups of patients. The results show that the value of OSDI for patients with keratoconus was higher (41.07 � 23.68) than the corresponding value of the control group (14.63 � 9.27). Also, the patients with keratoconus present lower mean values for BUT and Schirmer tests comparatively with the cases from control group. The electron paramagnetic resonances spectra of the spin probe CAT16 in tear secretions obtained from patients revealed changes that can be related with ophthalmologic parameters.

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