A microscale spectrophotometric method for quantification of doxorubicin in exhaled breath condensate

2019 ◽  
Vol 11 (5) ◽  
pp. 648-653 ◽  
Author(s):  
Abolghasem Jouyban ◽  
Azam Samadi ◽  
Vahid Jouyban-Gharamaleki ◽  
Maryam Khoubnasabjafari
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Spectrophotometric Method ◽  
Localized Surface Plasmon Resonance ◽  
Exhaled Breath Condensate ◽  
Localized Surface Plasmon ◽  
Exhaled Breath ◽  
Resonance Property ◽  
Breath Condensate

This study presents a microscale spectrophotometric method for the determination of doxorubicin, one of the most important anticancer drugs in the clinic, based on the localized surface plasmon resonance property of silver nanoparticles.

scholarly journals A Localized Surface Plasmon Resonance Sensing Method for Simultaneous Determination of Atenolol and Amiloride in Pharmaceutical Dosage Forms and Urine Samples

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Marwa R. El-Zahry
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Simultaneous Determination ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Dosage Forms ◽  
Localized Surface Plasmon ◽  
Pharmaceutical Dosage Forms

This contribution describes a simple, fast, and sensitive application of localized surface plasmon resonance effect of silver nanoparticles for simultaneous determination of antihypertensive drugs’ mixture atenolol and amiloride in both pharmaceutical dosage forms and in biological samples (urine). Silver nanoparticles were prepared by chemical reduction of silver nitrate using hydroxylamine HCL in an alkaline medium. Application of silver-hydroxylamine nanoparticles (SH NPs) provides many advantages including reproducibility, sensitivity, and cost effective way of analyte determination. Amiloride has four amino groups which act as attachment points on the surface of silver nanoparticles resulting in a synergistic effect on the absorption intensity of atenolol, leading to increase the sensitivity of the determination of both compounds. This method shows excellent advantages comparing with the previously reported methods, including accuracy, precision, and selectivity. The linear range of atenolol is 1 × 10−5–1 × 10−4 mol·L−1 and of amiloride is 1 × 10−6–1 × 10−5 mol·L−1. The limit of detection (LOD) values of atenolol and amiloride are 0.89 × 10−5 and 0.42 × 10−6 mol·L−1, respectively.

Amidosulfonic acid-capped silver nanoparticles for the spectrophotometric determination of lamotrigine in exhaled breath condensate

2017 ◽  
Vol 184 (8) ◽  
pp. 2991-2998 ◽  
Author(s):  
Abolghasem Jouyban ◽  
Azam Samadi ◽  
Maryam Khoubnasabjafari ◽  
Vahid Jouyban-Gharamaleki ◽  
Fatemeh Ranjbar
Keyword(s):  
Silver Nanoparticles ◽  
Spectrophotometric Determination ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Breath Condensate

Simultaneous and Sensitive Determination of Amphetamine, Codeine and Morphine in Exhaled Breath Condensate, Using Capillary Electrophoresis Coupled with On-line and Off-line Enhancing Methods

2020 ◽  
Vol 16 (7) ◽  
pp. 872-879
Author(s):  
Samin Hamidi
Keyword(s):  
Drugs Of Abuse ◽  
Exhaled Breath Condensate ◽  
Direct Injection ◽  
Forensic Toxicology ◽  
Analytical Signal ◽  
Exhaled Breath ◽  
Concentration Method ◽  
Validation Parameters ◽  
Breath Condensate

Background: Abuse of drugs is associated with several medical, forensic, toxicology and social challenges. “Drugs of abuse” testing is therefore an important issue. Objective: We propose a simple CE-based method for the quantification of amphetamine, codeine and morphine after direct injection of Exhaled Breath Condensate (EBC) by the aid of simple stacking mode and an off-line pre-concentration method. Methods: Using graphene oxide adsorbents, amphetamine, codeine and morphine were extracted from EBC in order to eliminate the proteins and other interferences. In addition to off-line method, an online stacking mode was applied to improve the analytical signal obtained from the instrument. Results: The validation parameters were experimented on the developed method based on the FDA guideline over concentration ranges of 12.5-100, 30-500 and 10-1250 ng/mL associated with amphetamine, codeine and morphine, respectively. Small volumes (around 100 μL) of EBC were collected using a lab-made setup and successfully analyzed using the proposed method where precisions and accuracies (within day and between days) were in accordance with the guideline (recommended less than 15 % for biological samples). The recovery tests were used to evaluate the matrix effect and data (94 to 105 %) showed that the proposed method can be applied in different EBC matrix samplings of subjects. Conclusion: The proposed method is superior for simultaneous determination of amphetamine, codeine and morphine over chromatographic analyses because it is fast and consumes fewer chemicals, with no derivatization step.

Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate

2020 ◽  
Vol 16 (8) ◽  
pp. 1032-1040
Author(s):  
Laleh Samini ◽  
Maryam Khoubnasabjafari ◽  
Mohamad M. Alimorad ◽  
Vahid Jouyban-Gharamaleki ◽  
Hak-Kim Chan ◽  
...  
Keyword(s):  
Biological Fluids ◽  
Exhaled Breath Condensate ◽  
Low Cost ◽  
Exhaled Breath ◽  
Chromatography Method ◽  
Extraction Solvent ◽  
Drug Concentrations ◽  
Dispersive Liquid Liquid Microextraction ◽  
Breath Condensate

Background: Analysis of drug concentrations in biological fluids is required in clinical sciences for various purposes. Among other biological samples, exhaled breath condensate (EBC) is a potential sample for follow up of drug concentrations. Methods: A dispersive liquid-liquid microextraction (DLLME) procedure followed by a validated liquid chromatography method was employed for the determination of budesonide (BDS) in EBC samples collected using a homemade setup. EBC is a non-invasive biological sample with possible applications for monitoring drug concentrations. The proposed analytical method is validated according to the FDA guidelines using EBC-spiked samples. Its applicability is tested on EBC samples collected from healthy volunteers receiving a single puff of BDS. Results: The best DLLME conditions involved the use of methanol (1 mL) as a disperser solvent, chloroform (200 μL) as an extraction solvent, and centrifugation rate of 3500 rpm for 5 minutes. The method was validated over a concentration range of 21-210 μg·L-1 in EBC. Inter- and intra-day precisions were less than 10% where the acceptable levels are less than 20%. The validated method was successfully applied for the determination of BDS in EBC samples. Conclusion: The findings of this study indicate that the developed method can be used for the extraction and quantification of BDS in EBC samples using a low cost method.

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