Fluorescence ELISA based on CAT-regulated fluorescence quenching of CdTe QDs for sensitive detection of FB1

2018 ◽  
Vol 10 (48) ◽  
pp. 5797-5802 ◽  
Author(s):  
Tianying Lu ◽  
Shengnan Zhan ◽  
Yaofeng Zhou ◽  
Xirui Chen ◽  
Xiaolin Huang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Quenching ◽  
Immunosorbent Assay ◽  
Cdte Quantum Dots ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mercaptopropionic Acid ◽  
Cdte Qds ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A competitive fluorescence enzyme-linked immunosorbent assay (cFELISA) was developed for the highly sensitive detection of fumonisin B1 (FB1) based on the catalase (CAT)-regulated fluorescence quenching of mercaptopropionic acid-modified CdTe quantum dots (MPA-QDs).

scholarly journals A rapid and highly sensitive biomarker detection platform based on a temperature-responsive liposome-linked immunosorbent assay

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Runkai Hu ◽  
Keitaro Sou ◽  
Shinji Takeoka
Keyword(s):  
Immunosorbent Assay ◽  
Limit Of Detection ◽  
Specific Antigen ◽  
Fluorescence Emission ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Temperature Responsive ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Abstract The enzyme-linked immunosorbent assay (ELISA) is widely used in various fields to detect specific biomarkers. However, ELISA tests have limited detection sensitivity (≥ 1 pM), which is insufficiently sensitive for the detection of small amounts of biomarkers in the early stages of disease or infection. Herein, a method for the rapid and highly sensitive detection of specific antigens, using temperature-responsive liposomes (TLip) containing a squaraine dye that exhibits fluorescence at the phase transition temperature of the liposomes, was developed. A proof-of-concept study using biotinylated TLip and a streptavidin-immobilized microwell plate showed that the TLip bound to the plate via specific molecular recognition could be distinguished from unbound TLip within 1 min because of the difference in the heating time required for the fluorescence emission of TLip. This system could be used to detect prostate specific antigen (PSA) based on a sandwich immunosorbent assay using detection and capture antibodies, in which the limit of detection was as low as 27.6 ag/mL in a 100-μL PSA solution, 0.97 aM in terms of molar concentration. The present temperature-responsive liposome-linked immunosorbent assay provides an advanced platform for the rapid and highly sensitive detection of biomarkers for use in diagnosis and biological inspections.

Capillary-based enzyme-linked immunosorbent assay for highly sensitive detection of thrombin-cleaved osteopontin in plasma

2013 ◽  
Vol 440 (2) ◽  
pp. 137-141 ◽  
Author(s):  
Shun-ichi Funano ◽  
Terence G. Henares ◽  
Mie Kurata ◽  
Kenji Sueyoshi ◽  
Tatsuro Endo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Highly sensitive detection of lead(II) ion using multicolor CdTe quantum dots

2011 ◽  
Vol 176 (1-2) ◽  
pp. 101-107 ◽  
Author(s):  
Wenying Zhong ◽  
Cui Zhang ◽  
Qin Gao ◽  
Hui Li
Keyword(s):  
Quantum Dots ◽  
Cdte Quantum Dots ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection
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