Highly sensitive colorimetric immunoassay for Escherichia coli O157:H7 based on probe of pseudo enzyme and dual signal amplification

2018 ◽  
Vol 10 (35) ◽  
pp. 4301-4309 ◽  
Author(s):  
Haoran Huang ◽  
Zunyi Wu ◽  
Jianfeng Huang ◽  
Guangying Zhao ◽  
Wenchao Dou
Keyword(s):  
Signal Amplification ◽  
Quantitative Detection ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Amplification System ◽  
Colorimetric Immunoassay ◽  
Dual Signal Amplification ◽  
Signal Amplification System

A sensitive colorimetric immunoassay was established for quantitative detection of E. coli O157:H7. Au@Pt with peroxidase-like activity was combined with flaky reduced graphene oxide to form a chromogenic probe of pseudo enzyme. HRP was modified on rGO-NR–Au@Pt and a dual signal amplification system was realized.

A highly sensitive electrochemical aptasensor for detection of microcystin-LR based on a dual signal amplification strategy

The Analyst ◽  
2019 ◽  
Vol 144 (5) ◽  
pp. 1671-1678 ◽  
Author(s):  
Xiaoqiang Liu ◽  
Yunfei Tang ◽  
Peipei Liu ◽  
Liwei Yang ◽  
Lele Li ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Signal Amplification ◽  
Electrochemical Aptasensor ◽  
Ternary Composite ◽  
Highly Sensitive ◽  
Amplification System ◽  
Amplification Strategy ◽  
Dual Signal Amplification ◽  
Signal Amplification System

In this work, a sensitive and selective electrochemical aptasensor for detection of microcystin-LR was developed based on a dual signal amplification system consisted of a novel ternary composite and horseradish peroxidase.

A novel electrochemical immunosensor based on dual signal amplification of gold nanoparticles and telomerase extension reaction

2014 ◽  
Vol 6 (7) ◽  
pp. 2221-2226 ◽  
Author(s):  
Xiao-Yan Li ◽  
Zi Yi ◽  
Hao Tang ◽  
Xia Chu ◽  
Ru-Qin Yu
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Highly Sensitive ◽  
Dual Signal Amplification

Highly sensitive electrochemical immune analysis was achieved based on dual signal amplification of AuNPs and telomerase extension reaction.

Electrochemical biosensor for detection of pathogenic bacteria based on dual signal amplification of Cu3(PO4)2-mediated click chemistry and DNAzyme

The Analyst ◽  
2021 ◽  
Author(s):  
Hongguo Wei ◽  
Jiayu Wan ◽  
Shengjun Bu ◽  
Wenguang Zhang ◽  
Ma Li ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Magnetic Separation ◽  
Concanavalin A ◽  
Signal Amplification ◽  
Pathogenic Bacteria ◽  
Electrochemical Biosensor ◽  
Inorganic Hybrid ◽  
Highly Sensitive ◽  
Antibody Conjugates ◽  
Dual Signal Amplification

A novel electrochemical biosensor for detecting pathogenic bacteria was designed based on specific magnetic separation and highly sensitive click chemistry. Instead of enzyme-antibody conjugates, organic-inorganic hybrid nanoflowers (Concanavalin A (Con...

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