Universally applicable three-dimensional hydrodynamic focusing in a single-layer channel for single cell analysis

2018 ◽  
Vol 10 (28) ◽  
pp. 3489-3497 ◽  
Author(s):  
Yingying Zhao ◽  
Qin Li ◽  
Xiaoming Hu
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Single Layer ◽  
Optical Systems ◽  
Cell Analysis ◽  
Hydrodynamic Focusing ◽  
Integrated Optical ◽  
Microfluidic Structure

A microfluidic cytometer which integrated 3D hydrodynamic focusing and integrated optical systems on a single-layer microfluidic structure was demonstrated.

scholarly journals Advances in Single-Cell Printing

Micromachines ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 80
Author(s):  
Xiaohu Zhou ◽  
Han Wu ◽  
Haotian Wen ◽  
Bo Zheng
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Cell Isolation ◽  
Special Focus ◽  
Cell Analysis ◽  
Cell Array ◽  
Cell Printing ◽  
Recent Developments ◽  
Single Cell Isolation

Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.

Densely-Packed Microbowl Array with Balanced Dielectrophoretic Forces for Single-Cell Microarray

2009 ◽  
Vol 1222 ◽  
Author(s):  
Maesoon Im ◽  
Dong-Haan Kim ◽  
Joo-Hyung Lee ◽  
Jun-Bo Yoon ◽  
Yang-Kyu Choi
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Average Diameter ◽  
Experimental Result ◽  
Cell Analysis ◽  
Large Area ◽  
Latex Beads ◽  
Nickel Electroplating ◽  
Cell Microarray

AbstractIn this paper, we demonstrate a perfectly-ordered microbowl array with balanced dielectrophoresis (DEP) for a high-throughput single-cell analysis. In order to fabricate well-ordered microbowl array in a large area, we utilized three-dimensional diffuser lithography for photoresist mold and nickel electroplating technique for final microbowl structures on a silicon substrate. Single microbowl has six sharp apexes surrounding the microbowl perimeter. Each microbowl has a diameter of 10 μm, and a height of 9 μm, which can be controllable by patterns on mask and lithography conditions. To investigate feasibility for application to the microbowl array as a single-cell microarray, we used latex beads of 6.4 μm in an average diameter to be captured by dielectrophoretic force. The nickel microbowl array densely packed with a hexagonal geometry played as a bottom electrode, and an ITO-coated glass covered the nickel microbowl array as a top electrode while keeping a uniform gap between two electrodes. After injecting solution containing latex beads through the gap, we applied an AC signal (2 VPP, 1 MHz) between two electrodes to induce high electric field near the sharp apexes of the single microbowl. A negative DEP trap is formed at the center of the single microbowl with balanced DEP force from the six apexes. The experimental result shows that injected latex beads had been successfully and uniformly aligned and trapped at the microbowl array sustained by negative DEP.

Three Dimensional Manipulation of Cells Using Holographic Optical Tweezers

2012 ◽  
Vol 241-244 ◽  
pp. 513-516
Author(s):  
Tao Tao ◽  
Jing Li ◽  
Yang Lin
Keyword(s):  
Single Cell ◽  
Optical Tweezers ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Optic Axis ◽  
Three Dimensions ◽  
Yeast Cells ◽  
Optical Traps ◽  
Cell Analysis ◽  
Holographic Optical Tweezers

A holographic optical tweezers platform was built and a multi-plane adaptive-additive algorithm was used to generating holograms for the reconstruction of optical traps in three-dimensional (3D) spaces. Experiments of manipulating cells were conducted on such platform and complex 3D structures were built with yeast cells. The results demonstrate that holographic optical tweezers can manipulate groups of cells in three dimensions and effectively trap and separate cells in the direction of the optic axis without harming the cells. Based on those versatile functions, it is proved that holographic optical tweezers is a powerful tool for single cell analysis.

Author response for "Immunocyte single cell analysis of vaccine‐induced narcolepsy"

2020 ◽  
Author(s):  
Alexander Lind ◽  
Falastin Salami ◽  
Anne‐Marie Landtblom ◽  
Lars Palm ◽  
Åke Lernmark ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Author Response ◽  
Cell Analysis
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