A versatile matrix of an ionic liquid functionalized with aldehyde and ferrocene groups for label-free electrochemical immunosensors

2018 ◽  
Vol 10 (14) ◽  
pp. 1612-1617 ◽  
Author(s):  
Youming Shen ◽  
Guangyu Shen ◽  
Youyu Zhang
Keyword(s):  
Ionic Liquid ◽  
Label Free ◽  
Electrochemical Immunosensors ◽  
Aldehyde Groups

A new “all-in-one” ionic liquid anchored with both Fc and aldehyde groups for immunoassay applications.

Electrochemical Immunosensors Based on Nanostructured Materials for Sensing of Prostate-Specific Antigen: A Review

2020 ◽  
Vol 28 ◽  
Author(s):  
Hayati Filik ◽  
Asiye Aslıhan Avan ◽  
Mustafa Özyürek
Keyword(s):  
Prostate Specific Antigen ◽  
Nanostructured Materials ◽  
Specific Antigen ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Electrochemical Immunosensors ◽  
Metal Metal ◽  
Different Types ◽  
Sandwich Type ◽  
Carbon Based Nanomaterials

: The prostate-specific antigen (PSA) has been considered a crucial serological marker for distinguishing prostate based cancer. This surveys recent progress in the construction of nanomaterial-based electrochemical immunosensors for a PSA. This review (from 2015 to 2020) reports the latest progress in PSA sensing based on the employ of different types of nanostructured materials. The most popular used nanostructured materials are metal, metal oxide, carbon-based nanomaterials, and their hybrid architectures utilized for distinct amplification protocols. In this review, the electrochemical immunosensors for prostate-specific antigen sensing are classified into three categories such as sandwich type@labeled, label free@nonlabeled and aptamer-based electrochemical immunosensor.

Designing label-free electrochemical immunosensors for cytochrome c using nanocomposites functionalized screen printed electrodes

2014 ◽  
Vol 54 ◽  
pp. 115-121 ◽  
Author(s):  
Manickam Pandiaraj ◽  
Niroj Kumar Sethy ◽  
Kalpana Bhargava ◽  
Vepa Kameswararao ◽  
Chandran Karunakaran
Keyword(s):  
Cytochrome C ◽  
Label Free ◽  
Screen Printed Electrodes ◽  
Electrochemical Immunosensors ◽  
Screen Printed

Synthesis of fluorescent ionic liquid-functionalized silicon nanoparticles with tunable amphiphilicity and selective determination of Hg2+

2018 ◽  
Vol 6 (48) ◽  
pp. 8214-8220 ◽  
Author(s):  
Quan Li ◽  
Kaite Peng ◽  
Yanzhen Lu ◽  
Aoxin Li ◽  
Fenfang Che ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Silicon Nanoparticles ◽  
Selective Detection ◽  
Label Free ◽  
Selective Determination ◽  
Highly Sensitive

Label-free fluorescent ionic liquid-functionalized silicon nanoparticles with tunable amphiphilicity for highly sensitive and selective detection of Hg2+ were synthesized.

3D Label-Free Matrix Metalloproteinase-3 Immunosensor Based on Graphene Oxide/Polypyrrole-Ionic Liquid Nanocomposite

2015 ◽  
Vol 7 (8) ◽  
pp. 1581-1588 ◽  
Author(s):  
Zhouyang Yin ◽  
Yingying Wang ◽  
Ting-Ting Zheng ◽  
Rui Zhang ◽  
Xinghua Li ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Graphene Oxide ◽  
Matrix Metalloproteinase ◽  
Label Free ◽  
Matrix Metalloproteinase 3

scholarly journals Molecular Dynamics Simulation-assisted Ionic Liquid Screening for Deep Coverage Proteome Analysis

2020 ◽  
Vol 19 (10) ◽  
pp. 1724-1737
Author(s):  
Fei Fang ◽  
Qun Zhao ◽  
Huiying Chu ◽  
Mingwei Liu ◽  
Baofeng Zhao ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Ionic Liquid ◽  
Molecular Dynamics Simulation ◽  
Membrane Proteins ◽  
Sample Preparation ◽  
Proteomic Analysis ◽  
Dynamics Simulation ◽  
Dominant Factor ◽  
Label Free ◽  
Compound Libraries

In-depth coverage of proteomic analysis could enhance our understanding to the mechanism of the protein functions. Unfortunately, many highly hydrophobic proteins and low-abundance proteins, which play critical roles in signaling networks, are easily lost during sample preparation, mainly attributed to the fact that very few extractants can simultaneously satisfy the requirements on strong solubilizing ability to membrane proteins and good enzyme compatibility. Thus, it is urgent to screen out ideal extractant from the huge compound libraries in a fast and effective way. Herein, by investigating the interior mechanism of extractants on the membrane proteins solubilization and trypsin compatibility, a molecular dynamics simulation system was established as complement to the experimental procedure to narrow down the scope of candidates for proteomics analysis. The simulation data shows that the van der Waals interaction between cation group of ionic liquid and membrane protein is the dominant factor in determining protein solubilization. In combination with the experimental data, 1-dodecyl-3-methylimidazolium chloride (C12Im-Cl) is on the shortlist for the suitable candidates from comprehensive aspects. Inspired by the advantages of C12Im-Cl, an ionic liquid-based filter-aided sample preparation (i-FASP) method was developed. Using this strategy, over 3,300 proteins were confidently identified from 103 HeLa cells (∼100 ng proteins) in a single run, an improvement of 53% over the conventional FASP method. Then the i-FASP method was further successfully applied to the label-free relative quantitation of human liver cancer and para-carcinoma tissues with obviously improved accuracy, reproducibility and coverage than the commonly used urea-based FASP method. The above results demonstrated that the i-FASP method could be performed as a versatile tool for the in-depth coverage proteomic analysis of biological samples.

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