Fast simultaneous detection of three diterpenoids in Herba Siegesbeckiae using solid phase extraction followed by HPLC-UV with a core–shell particle column

2018 ◽  
Vol 10 (11) ◽  
pp. 1325-1330 ◽  
Author(s):  
Yong Zhang ◽  
Zhizhong Zou ◽  
Guixing Chou
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Extraction Methods ◽  
Core Shell ◽  
Phase Extraction ◽  
Shell Particle

To quickly determine the amounts of kirenol, darutoside and darutigenol in Herba Siegesbeckiae using HPLC, the efficiency of sample cleanup with different solid phase extraction methods was systematically investigated.

scholarly journals NanoMIP-Based Solid Phase Extraction of Fluoroquinolones from Human Urine: A Proof-of-Concept Study

Separations ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 226
Author(s):  
Matteo Chiarello ◽  
Laura Anfossi ◽  
Simone Cavalera ◽  
Fabio Di Di Nardo ◽  
Thea Serra ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Human Urine ◽  
Solid Phase ◽  
Extraction Methods ◽  
Hplc Method ◽  
Limited Attention ◽  
Preliminary Treatment ◽  
Phase Extraction ◽  
Proof Of Concept ◽  
Binding Behavior

NanoMIPs that are prepared by solid phase synthesis have proven to be very versatile, but to date only limited attention has been paid to their use in solid phase extraction. Thus, since nanoMIPs show close similarities, in terms of binding behavior, to antibodies, it seems relevant to verify if it is possible to use them as mimics of the natural antibodies that are used in immunoextraction methods. As a proof-of-concept, we considered prepared nanoMIPs against fluoroquinolone ciprofloxacin. Several nanoMIPs were prepared in water with polymerization mixtures of different compositions. The polymer with the highest affinity towards ciprofloxacin was then grafted onto a solid support and used to set up a solid phase extraction–HPLC method with fluorescence detection, for the determination of fluoroquinolones in human urine. The method resulted in successful selection for the fluoroquinolone antibiotics, such that the nanoMIPs were suitable for direct extraction of the antibiotics from the urine samples at the µg mL−1 level. They required no preliminary treatment, except for a 1 + 9 (v/v) dilution with a buffer of pH 4.5 and they had good analyte recovery rates; up to 85% with precision in the range of 3 to 4.5%, without interference from the matrix. These experimental results demonstrate, for the first time, the feasibility of the use of nanoMIPs to develop solid phase extraction methods.

scholarly journals Modern Approaches to Preparation of Body Fluids for Determination of Bioactive Compounds

Separations ◽  
2019 ◽  
Vol 6 (4) ◽  
pp. 53 ◽  
Author(s):  
Katarzyna Madej ◽  
Wojciech Piekoszewski
Keyword(s):  
Liquid Phase ◽  
Solid Phase Extraction ◽  
Bioactive Compounds ◽  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Extraction Methods ◽  
Body Fluids ◽  
Phase Extraction ◽  
Modern Approach ◽  
Dispersive Liquid Liquid Microextraction

The current clinical and forensic toxicological analysis of body fluids requires a modern approach to sample preparation characterized by high selectivity and enrichment capability, suitability for micro-samples, simplicity and speed, and the possibility of automation and miniaturization, as well as the use of small amounts of reagents, especially toxic solvents. Most of the abovementioned features may be realized using so-called microextraction techniques which cover liquid-phase techniques (e.g., single-drop microextraction, SDME; dispersive liquid–liquid microextraction, DLLME; hollow-fiber liquid-phase microextraction, HF-LPME) and solid-phase extraction techniques (solid-phase microextraction, SPME; microextraction in packed syringes, MEPS; disposable pipette tip extraction, DPX; stir bar sorption extraction, SBSE). Some other extraction methodologies like dispersive solid-phase extraction (d-SPE) or magnetic solid-phase extraction (MSPE) can also be easily miniaturized. This review briefly describes and characterizes the abovementioned extraction methods, and then presents their current applications to the preparation of body fluids analyzed for bioactive compounds in combination with appropriate analytical methods, mainly chromatographic and related techniques. The perspectives of the analytical area we are interested in are also indicated.

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