scholarly journals Fluorescence lifetime imaging of intracellular magnesium content in live cells

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1876-1880 ◽  
Author(s):  
Azzurra Sargenti ◽  
Alessia Candeo ◽  
Giovanna Farruggia ◽  
Cosimo D'Andrea ◽  
Concettina Cappadone ◽  
...  
Keyword(s):  
Detailed Analysis ◽  
Fluorescence Lifetime ◽  
Cell Imaging ◽  
Magnesium Content ◽  
Live Cells ◽  
Fluorescence Lifetime Imaging ◽  
Intracellular Magnesium ◽  
Lifetime Imaging

The first detailed analysis for Mg cell imaging by visible FLIM is presented.

scholarly journals Streamlined histone-based fluorescence lifetime imaging microscopy reveals ATM regulation of chromatin compaction

2017 ◽  
Author(s):  
Alice Sherrard ◽  
Paul Bishop ◽  
Melanie Panagi ◽  
Maria Beatriz Villagomez ◽  
Dominic Alibhai ◽  
...  
Keyword(s):  
Dna Damage ◽  
Fluorescence Lifetime ◽  
Genotoxic Stress ◽  
Live Cells ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Chromatin Compaction ◽  
Lifetime Imaging ◽  
Detailed Method ◽  
Chromatin Organisation

AbstractChanges in chromatin compaction are crucial during genomic responses. Thus, methods that enable such measurements are instrumental for investigating genome function. Here, we address this challenge by developing, validating, and streamlining histone-based fluorescence lifetime imaging microscopy (FLIM) that robustly detects chromatin compaction states in fixed and live cells; in 2D and 3D. We present quality-controlled and detailed method that is simpler and faster than previous approches, and uses FLIMfit open-source software. We demonstrate the versatility of our method through its combination with immunofluorescence and its implementation in immortalised cells and primary neurons. Owing to these developments, we applied this method to elucidate the function of the DNA damage response kinase, ATM, in regulating chromatin organisation after genotoxic-stress. We unravelled a role for ATM in regulating chromatin compaction independently of DNA damage. Collectively, we present an adaptable chromatin FLIM method for examining chromatin structure in cells, and establish its broader utility.

Spectroscopy and fluorescence lifetime imaging in live cells of a cyano-substituted combretastatin

2014 ◽  
Vol 3 (3) ◽  
pp. 211-218 ◽  
Author(s):  
Kathrin M. Scherer ◽  
Roger H. Bisby ◽  
Stanley W. Botchway ◽  
Greg M. Greetham ◽  
John A. Hadfield ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Live Cells ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Fluorescence axial nanotomography with plasmonics

2015 ◽  
Vol 178 ◽  
pp. 371-381 ◽  
Author(s):  
Nicholas I. Cade ◽  
Gilbert O. Fruhwirth ◽  
Alexey V. Krasavin ◽  
Tony Ng ◽  
David Richards
Keyword(s):  
Fluorescence Lifetime ◽  
Cell Imaging ◽  
Super Resolution ◽  
Cellular Membrane ◽  
Carcinoma Cells ◽  
Axial Position ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Position Sensitivity ◽  
Confocal Fluorescence

We present a novel imaging technique with super-resolution axial sensitivity, exploiting the changes in fluorescence lifetime above a plasmonic substrate. Using conventional confocal fluorescence lifetime imaging, we show that it is possible to deliver down to 6 nm axial position sensitivity of fluorophores in whole biological cell imaging. We employ this technique to map the topography of the cellular membrane, and demonstrate its application in an investigation of receptor-mediated endocytosis in carcinoma cells.

scholarly journals Multiplexable fluorescence lifetime imaging (FLIM) probes for Abl and Src-family kinases

2020 ◽  
Vol 56 (87) ◽  
pp. 13409-13412
Author(s):  
Sampreeti Jena ◽  
Nur P. Damayanti ◽  
Jackie Tan ◽  
Erica D. Pratt ◽  
Joseph M. K. Irudayaraj ◽  
...  
Keyword(s):  
Single Cell ◽  
Fluorescence Lifetime ◽  
Src Family Kinases ◽  
Live Cells ◽  
Cell Penetrating Peptide ◽  
Fluorescence Lifetime Imaging ◽  
Src Family Kinase ◽  
Peptide Substrates ◽  
Lifetime Imaging ◽  
Cell Penetrating

Here we describe development of cell-penetrating peptide substrates for fluorescence lifetime imaging (FLIM) of Abl and Src-family kinase activities, applied to mapping single cell and subcellular dynamics of multiple kinases in live cells.

scholarly journals Fluorescence lifetime imaging of physiological free Cu(ii) levels in live cells with a Cu(ii)-selective carbonic anhydrase-based biosensor

Metallomics ◽  
2014 ◽  
Vol 6 (5) ◽  
pp. 1034 ◽  
Author(s):  
Bryan J. McCranor ◽  
Henryk Szmacinski ◽  
Hui Hui Zeng ◽  
Andrea K. Stoddard ◽  
Tamiika Hurst ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Fluorescence Lifetime ◽  
Live Cells ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging
Sign in / Sign up

Export Citation Format

Share Document