A cascade toehold-mediated strand displacement strategy for label-free and sensitive non-enzymatic recycling amplification detection of the HIV-1 gene

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 2173-2178 ◽  
Author(s):  
Qiong Li ◽  
Zhi Liu ◽  
Danhua Zhou ◽  
Jiafeng Pan ◽  
Chengshuai Liu ◽  
...  
Keyword(s):  
Label Free ◽  
Strand Displacement ◽  
Gene Detection ◽  
Displacement Reactions ◽  
Recycling Amplification ◽  
Hiv 1

A label-free biosensor was proposed for HIV-1 gene detection based on cascade toehold-mediated strand displacement reactions.

An efficient target–intermediate recycling amplification strategy for ultrasensitive fluorescence assay of intracellular lead ions

2017 ◽  
Vol 53 (54) ◽  
pp. 7525-7528 ◽  
Author(s):  
Zhi-Bin Wen ◽  
Wen-Bin Liang ◽  
Ying Zhuo ◽  
Cheng-Yi Xiong ◽  
Ying-Ning Zheng ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Lead Ions ◽  
Strand Displacement ◽  
Displacement Reactions ◽  
Intracellular Lead ◽  
Recycling Amplification ◽  
Amplification Strategy

An ultrasensitive fluorescence assay for intracellular Pb2+ determination was proposed through target–intermediate recycling amplification based on metal-assisted DNAzyme catalysis and strand displacement reactions.

Label-free amplified fluorescence detection of DNA biomarkers based on KFP polymerase-driven double strand displacement reactions and magnetic nanoprobes

2020 ◽  
Vol 12 (24) ◽  
pp. 3092-3097
Author(s):  
Xia Sun ◽  
Yeling Liu ◽  
Liqi Liu ◽  
Fei Yin ◽  
Ruixin Liu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Label Free ◽  
Strand Displacement ◽  
Displacement Reactions

Schematic illustration of the label-free amplified fluorescence detection of the P53 DNA based on KFP polymerase-driven double strand displacement reactions and magnetic nanoprobe.

Biocomputing based on DNA strand displacement reactions

ChemPhysChem ◽  
2021 ◽  
Author(s):  
Hui Lv ◽  
Qian Li ◽  
Jiye Shi ◽  
Fei Wang ◽  
Chunhai Fan
Keyword(s):  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand

scholarly journals Surface Plasmon Resonance Assay for Label-Free and Selective Detection of HIV-1 p24 Protein

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 180
Author(s):  
Lucia Sarcina ◽  
Giuseppe Felice Mangiatordi ◽  
Fabrizio Torricelli ◽  
Paolo Bollella ◽  
Zahra Gounani ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Covalent Binding ◽  
Hiv Infections ◽  
Selectivity Ratio ◽  
Selective Detection ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Label Free Detection ◽  
P24 Protein ◽  
Hiv 1

The early detection of the human immunodeficiency virus (HIV) is of paramount importance to achieve efficient therapeutic treatment and limit the disease spreading. In this perspective, the assessment of biosensing assay for the HIV-1 p24 capsid protein plays a pivotal role in the timely and selective detection of HIV infections. In this study, multi-parameter-SPR has been used to develop a reliable and label-free detection method for HIV-1 p24 protein. Remarkably, both physical and chemical immobilization of mouse monoclonal antibodies against HIV-1 p24 on the SPR gold detecting surface have been characterized for the first time. The two immobilization techniques returned a capturing antibody surface coverage as high as (7.5 ± 0.3) × 1011 molecule/cm2 and (2.4 ± 0.6) × 1011 molecule/cm2, respectively. However, the covalent binding of the capturing antibodies through a mixed self-assembled monolayer (SAM) of alkanethiols led to a doubling of the p24 binding signal. Moreover, from the modeling of the dose-response curve, an equilibrium dissociation constant KD of 5.30 × 10−9 M was computed for the assay performed on the SAM modified surface compared to a much larger KD of 7.46 × 10−5 M extracted for the physisorbed antibodies. The chemically modified system was also characterized in terms of sensitivity and selectivity, reaching a limit of detection of (4.1 ± 0.5) nM and an unprecedented selectivity ratio of 0.02.

Target-induced structure switching of DNA for label-free and ultrasensitive electrochemiluminescent detection of proteins

2014 ◽  
Vol 50 (24) ◽  
pp. 3211-3213 ◽  
Author(s):  
Mengli Yang ◽  
Ying Chen ◽  
Yun Xiang ◽  
Ruo Yuan ◽  
Yaqin Chai
Keyword(s):  
Dna Structure ◽  
Label Free ◽  
Switching Strategy ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Exo Iii ◽  
Recycling Amplification ◽  
Structure Switching ◽  
Induced Structure

Highly sensitive and label-free detection of thrombin is achieved via a target-induced DNA structure switching strategy and Exo III-assisted recycling amplification.

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