Crossing constriction channel-based microfluidic cytometry capable of electrically phenotyping large populations of single cells

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 1008-1015 ◽  
Author(s):  
Yi Zhang ◽  
Yang Zhao ◽  
Deyong Chen ◽  
Ke Wang ◽  
Yuanchen Wei ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Cells ◽  
Microfluidic System ◽  
Membrane Capacitance ◽  
Large Populations ◽  
Specific Membrane

This paper presents a crossing constriction channel-based microfluidic system for high-throughput characterization of specific membrane capacitance (Csm) and cytoplasm conductivity (σcy) of single cells.

scholarly journals Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System

Sensors ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 2763-2773 ◽  
Author(s):  
Yang Zhao ◽  
Deyong Chen ◽  
Yana Luo ◽  
Feng Chen ◽  
Xiaoting Zhao ◽  
...  
Keyword(s):  
Young’S Modulus ◽  
Young's Modulus ◽  
Single Cells ◽  
Microfluidic System ◽  
Membrane Capacitance ◽  
Specific Membrane

scholarly journals An acoustic platform for single-cell, high-throughput measurements of the viscoelastic properties of cells

2020 ◽  
Author(s):  
Valentin Romanov ◽  
Giulia Silvani ◽  
Huiyu Zhu ◽  
Charles D Cox ◽  
Boris Martinac
Keyword(s):  
Mechanical Properties ◽  
Single Cell ◽  
High Throughput ◽  
Single Cells ◽  
Adherent Cells ◽  
Dependent Manner ◽  
Cellular Processes ◽  
Membrane Cytoskeleton ◽  
Change Temperature

ABSTRACTCellular processes including adhesion, migration and differentiation are governed by the distinct mechanical properties of each cell. Importantly, the mechanical properties of individual cells can vary depending on local physical and biochemical cues in a time-dependent manner resulting in significant inter-cell heterogeneity. While several different methods have been developed to interrogate the mechanical properties of single cells, throughput to capture this heterogeneity remains an issue. While new high-throughput techniques are slowly emerging, they are primarily aimed at characterizing cells in suspension, whereas high-throughput measurements of adherent cells have proven to be more challenging. Here, we demonstrate single-cell, high-throughput characterization of adherent cells using acoustic force spectroscopy. We demonstrate that cells undergo marked changes in viscoelasticity as a function of temperature, the measurements of which are facilitated by a closed microfluidic culturing environment that can rapidly change temperature between 21 °C and 37 °C. In addition, we show quantitative differences in cells exposed to different pharmacological treatments specifically targeting the membrane-cytoskeleton interface. Further, we utilize the high-throughput format of the AFS to rapidly probe, in excess of 1000 cells, three different cell-lines expressing different levels of a mechanosensitive protein, Piezo1, demonstrating the ability to differentiate between cells based on protein expression levels.

Microfluidic characterization of specific membrane capacitance and cytoplasm conductivity of singlecells

2013 ◽  
Vol 42 ◽  
pp. 496-502 ◽  
Author(s):  
Yi Zheng ◽  
Ehsan Shojaei-Baghini ◽  
Chen Wang ◽  
Yu Sun
Keyword(s):  
Membrane Capacitance ◽  
Specific Membrane
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