A multiplex droplet digital PCR assay for non-invasive prenatal testing of fetal aneuploidies

The Analyst ◽  
2019 ◽  
Vol 144 (7) ◽  
pp. 2239-2247 ◽  
Author(s):  
Chianru Tan ◽  
Xihua Chen ◽  
Fang Wang ◽  
Dong Wang ◽  
Zongfu Cao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Prenatal Testing ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Locked Nucleic Acid ◽  
Pcr Assay ◽  
Nucleic Acid Probes ◽  
Non Invasive ◽  
Fetal Aneuploidies

Using universal locked nucleic acid probes, a high multiplexing ddPCR-based NIPT was developed to reliably identify fetal aneuploidies.

Interest of Droplet digital PCR in non-invasive prenatal testing

2015 ◽  
Vol 73 (S1) ◽  
pp. 22-23
Author(s):  
Lucie Orhant ◽  
Aurélie Vasson ◽  
France Leturcq ◽  
Dominique Vidaud ◽  
Thierry Bienvenu ◽  
...  
Keyword(s):  
Prenatal Testing ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Non Invasive

scholarly journals New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France

2006 ◽  
Vol 50 (4) ◽  
pp. 1594-1598 ◽  
Author(s):  
Jean-Winoc Decousser ◽  
Imen Methlouthi ◽  
Patrick Pina ◽  
Anne Collignon ◽  
Pierre Allouch
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nucleic Acid ◽  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
Locked Nucleic Acid ◽  
Pcr Assay ◽  
Nucleic Acid Probes ◽  
National Surveys ◽  
Risk Patients

ABSTRACT A real-time PCR assay with locked nucleic acid probes was developed to screen mutations at codons 79 and 83 of the Streptococcus pneumoniae parC gene. Only silent mutations were detected among 236 French invasive fluoroquinolone-susceptible strains. This test could be useful for some high-risk patients or in national surveys.

scholarly journals Non-Invasive Prenatal Testing: Current Perspectives and Future Challenges

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 15
Author(s):  
Luigi Carbone ◽  
Federica Cariati ◽  
Laura Sarno ◽  
Alessandro Conforti ◽  
Francesca Bagnulo ◽  
...  
Keyword(s):  
Prenatal Screening ◽  
Prenatal Testing ◽  
Non Invasive ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
Common Causes ◽  
Future Challenges ◽  
Neurodevelopmental Impairment ◽  
Fetal Aneuploidies ◽  
Made In

Fetal aneuploidies are among the most common causes of miscarriages, perinatal mortality and neurodevelopmental impairment. During the last 70 years, many efforts have been made in order to improve prenatal diagnosis and prenatal screening of these conditions. Recently, the use of cell-free fetal DNA (cff-DNA) testing has been increasingly used in different countries, representing an opportunity for non-invasive prenatal screening of pregnant women. The aim of this narrative review is to describe the state of the art and the main strengths and limitations of this test for prenatal screening of fetal aneuploidies.

scholarly journals Locked Nucleic Acid Probe-Based Real-Time PCR Assay for the Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143444 ◽  
Author(s):  
Yong Zhao ◽  
Guilian Li ◽  
Chongyun Sun ◽  
Chao Li ◽  
Xiaochen Wang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Locked Nucleic Acid ◽  
Pcr Assay ◽  
Locked Nucleic Acid Probe

Development of a sensitive and reliable reverse transcription droplet digital PCR assay for the detection of citrus yellow vein clearing virus

2018 ◽  
Vol 164 (3) ◽  
pp. 691-697 ◽  
Author(s):  
Yingjie Liu ◽  
Yingli Wang ◽  
Qin Wang ◽  
Yanhui Zhang ◽  
Wanxia Shen ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Yellow Vein ◽  
Pcr Assay ◽  
Vein Clearing

scholarly journals Droplet‐digital PCR assay to detect Merkel cell polyomavirus sequences in chorionic villi from spontaneous abortion affected females

2019 ◽  
Vol 235 (3) ◽  
pp. 1888-1894 ◽  
Author(s):  
Andrea Tagliapietra ◽  
John Charles Rotondo ◽  
Ilaria Bononi ◽  
Elisa Mazzoni ◽  
Federica Magagnoli ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Merkel Cell Polyomavirus ◽  
Merkel Cell ◽  
Pcr Assay ◽  
Chorionic Villi
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