scholarly journals A comparative study of branched and linear mannitol-based amphiphiles on membrane protein stability

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5702-5710 ◽  
Author(s):  
Hazrat Hussain ◽  
Tyler Helton ◽  
Yang Du ◽  
Jonas S. Mortensen ◽  
Parameswaran Hariharan ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Comparative Study ◽  
Protein Stability ◽  
Alkyl Chain ◽  
Alkyl Chains ◽  
Membrane Protein Stability ◽  
The Comparative Study

The comparative study on linear vs. branched alkyl-chain amphiphiles indicates a favorable role for branched alkyl-chains in stabilizing membrane proteins.

scholarly journals Isomeric Detergent Comparison for Membrane Protein Stability: Importance of Inter-Alkyl-Chain Distance and Alkyl Chain Length

ChemBioChem ◽  
2016 ◽  
Vol 17 (24) ◽  
pp. 2334-2339 ◽  
Author(s):  
Kyung Ho Cho ◽  
Parameswaran Hariharan ◽  
Jonas S. Mortensen ◽  
Yang Du ◽  
Anne K. Nielsen ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Stability ◽  
Chain Length ◽  
Alkyl Chain ◽  
Alkyl Chain Length ◽  
Membrane Protein Stability

scholarly journals Membrane Protein Stabilization Strategies for Structural and Functional Studies

Membranes ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 155
Author(s):  
Ekaitz Errasti-Murugarren ◽  
Paola Bartoccioni ◽  
Manuel Palacín
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Lipid Membrane ◽  
Protein Stabilization ◽  
New Drugs ◽  
Cell Physiology ◽  
Protein Preparation ◽  
Functional Studies ◽  
Membrane Protein Stability ◽  
Druggable Targets

Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies.

scholarly journals New Malonate-Derived Tetraglucoside Detergents for Membrane Protein Stability

2020 ◽  
Vol 15 (6) ◽  
pp. 1697-1707 ◽  
Author(s):  
Muhammad Ehsan ◽  
Satoshi Katsube ◽  
Cristina Cecchetti ◽  
Yang Du ◽  
Jonas S. Mortensen ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Stability ◽  
Membrane Protein Stability

scholarly journals Conformationally Restricted Monosaccharide-Cored Glycoside Amphiphiles: The Effect of Detergent Headgroup Variation on Membrane Protein Stability

2019 ◽  
Vol 14 (8) ◽  
pp. 1717-1726
Author(s):  
Aiman Sadaf ◽  
Manuel Ramos ◽  
Jonas S. Mortensen ◽  
Yang Du ◽  
Hyoung Eun Bae ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Stability ◽  
Conformationally Restricted ◽  
Membrane Protein Stability

scholarly journals Towards Understanding How Water Modulates Membrane Protein Stability

2020 ◽  
Vol 118 (3) ◽  
pp. 26a-27a
Author(s):  
Dagan C. Marx ◽  
Karen G. Fleming
Keyword(s):  
Membrane Protein ◽  
Protein Stability ◽  
Membrane Protein Stability

Membrane-protein stability in a phospholipid-based crystallization medium

2006 ◽  
Vol 154 (3) ◽  
pp. 223-231 ◽  
Author(s):  
Christopher S. Lunde ◽  
Shahab Rouhani ◽  
Marc T. Facciotti ◽  
Robert M. Glaeser
Keyword(s):  
Membrane Protein ◽  
Protein Stability ◽  
Membrane Protein Stability

scholarly journals Single-Molecule Measurement of Membrane Protein Stability

2016 ◽  
Vol 110 (3) ◽  
pp. 396a
Author(s):  
Robert Jefferson ◽  
Yu-Chu Chang ◽  
Eitan Lerner ◽  
Shimon Weiss ◽  
James Bowie
Keyword(s):  
Membrane Protein ◽  
Protein Stability ◽  
Single Molecule ◽  
Membrane Protein Stability

scholarly journals Methods for the solubilisation of membrane proteins: the micelle-aneous world of membrane protein solubilisation

2021 ◽  
Author(s):  
Giedre Ratkeviciute ◽  
Benjamin F. Cooper ◽  
Timothy J. Knowles
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Stability ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Modus Operandi ◽  
Membrane Mimetic ◽  
Aqueous Environments ◽  
Recent Developments

The solubilisation of membrane proteins (MPs) necessitates the overlap of two contradictory events; the extraction of MPs from their native lipid membranes and their subsequent stabilisation in aqueous environments. Whilst the current myriad of membrane mimetic systems provide a range of modus operandi, there are no golden rules for selecting the optimal pipeline for solubilisation of a specific MP hence a miscellaneous approach must be employed balancing both solubilisation efficiency and protein stability. In recent years, numerous diverse lipid membrane mimetic systems have been developed, expanding the pool of available solubilisation strategies. This review provides an overview of recent developments in the membrane mimetic field, with particular emphasis placed upon detergents, polymer-based nanodiscs and amphipols, highlighting the latest reagents to enter the toolbox of MP research.

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