Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 5979-5986 ◽  
Author(s):  
H. A. Owida ◽  
A. V. Rutter ◽  
G. Cinque ◽  
N. J. Kuiper ◽  
J. Sulé-Suso ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
3D Culture ◽  
Ftir Spectra ◽  
Pericellular Matrix ◽  
3 Dimensional ◽  
Spectroscopic Monitoring ◽  
Chondrocyte Culture ◽  
Culture Model ◽  
Fingerprint Region ◽  
Cultured Chondrocytes

PCA score of FTIR spectra in fingerprint region for cultured chondrocytes at day 7, 14 and 21 in HA hydrogel showing that the 3D culture drove chondrocytes (CY) to chondron-like (CN) structure dynamically.

Combination of proteasome inhibitors with temozolomide to increase the anti-tumor effect in 3D culture model of glioblastoma

2018 ◽  
Vol 120 ◽  
pp. S152
Author(s):  
Semra Unal ◽  
Tilbe Gokce ◽  
Sema Arslan ◽  
Ayse Mine Yilmaz ◽  
Oguzhan Gunduz ◽  
...  
Keyword(s):  
Proteasome Inhibitors ◽  
3D Culture ◽  
Culture Model ◽  
3D Culture Model

PNU-74654 enhanced the antiproliferative activity of gemcitabine via targeting Wnt/β-catenin Pathway in Pancreatic cancer

2021 ◽  
Author(s):  
Sajjad Sharifi ◽  
Farzad Rahmani ◽  
Abolfazl Nosrati-Tirkani ◽  
Shima Mehrabadi ◽  
Hamid Fiuji ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cell Culture ◽  
Wnt Pathway ◽  
3D Cell Culture ◽  
Current Therapy ◽  
Beta Catenin ◽  
Cell Culture Model ◽  
Novel Therapy ◽  
3 Dimensional ◽  
Culture Model

Abstract Background : The Wnt/beta-catenin pathway is dysregulated in pancreatic cancer and is reported to be associated with poor prognosis, indicating the need for identification of novel agents to improve the efficacy of current therapy or have better activity. Therefore in the present study we explored the anticancer activity of PNU-74654 alone or in combination with gemcitabine in 2 and 3 dimensional cell culture model of pancreatic cancer. Methods: The MTT assay was applied to determine the viability of PC cancerous cells (PCC), while the cytotoxicity of this agent was evaluated in 3D cell culture model (spheroid). The effects of PNU-74654 was investigated in established cell migration/invasion assays. Result: The expression of candidate genes affecting the cell cycle, migration, and Wnt/b-catenin pathway was evaluated at mRNA and/or proteins by RT-PCR or Western blot. PNU-74654 inhibited the cell growth at IC50 of 122±0.4 umol/L, and had a synergistic effect on the antiproliferative properties of gemcitabine by modulating the Wnt pathway. The PNU-74654/gemcitabine combination reduced the migratory and invasiveness of PC cells, compared to control cells through perturbation of E-cadherin. Conclusion: In aggregate our findings demonstrated the profound antitumor properties of PNU-74654 in pancreatic cancer, supporting further studies to evaluate the therapeutic impact of this novel therapy to target Wnt pathway in the treatment of pancreatic cancer.

Methyl-β-cyclodextrin treatment combined to incubation with interleukin-4 reproduces major features of atopic dermatitis in a 3D-culture model

2016 ◽  
Vol 309 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Tatiana do Nascimento Pedrosa ◽  
Evelyne De Vuyst ◽  
Abdallah Mound ◽  
Catherine Lambert de Rouvroit ◽  
Silvya Stuchi Maria-Engler ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
3D Culture ◽  
Interleukin 4 ◽  
Culture Model ◽  
3D Culture Model

scholarly journals Induced Tauopathy in a Novel 3D-Culture Model Mediates Neurodegenerative Processes: A Real-Time Study on Biochips

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e49150 ◽  
Author(s):  
Diana Seidel ◽  
Dana Krinke ◽  
Heinz-Georg Jahnke ◽  
Anika Hirche ◽  
Daniel Kloß ◽  
...  
Keyword(s):  
Real Time ◽  
3D Culture ◽  
Time Study ◽  
Culture Model ◽  
3D Culture Model

scholarly journals Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage

2016 ◽  
Vol 9 ◽  
pp. CMAMD.S29844
Author(s):  
Jessica Badendick ◽  
Owen Godkin ◽  
Benjamin Kohl ◽  
Carola Meier ◽  
Michal Jagielski ◽  
...  
Keyword(s):  
Complement Regulatory Proteins ◽  
Chondrocyte Phenotype ◽  
Chondrocyte Culture ◽  
In Vitro Characterization ◽  
Femoral Head Cartilage ◽  
Culture Characteristics ◽  
Histological Results ◽  
Cultured Chondrocytes

Osteoarthritis (OA) might affect chondrocyte culture characteristics and complement expression. Therefore, this study addressed the interrelation between macroscopical and microscopical structure, complement expression, and chondrocyte culture characteristics in non-OA and OA cartilage. Femoral head cartilage samples harvested from patients with femoral neck fractures (FNFs) and OA were analyzed for macroscopical alterations using an in-house scoring system, graded histologically (Mankin score), and immunolabeled for complement regulatory proteins (CRPs) and receptors. Morphology of monolayer cultured chondrocytes isolated from a subset of samples was assessed. The macroscopical score distinguished the FNF and OA cartilage samples and correlated significantly with the histological results. Chondrocyte phenotype from FNF or OA cartilage differed. Complement receptor C5aR, CRPs CD55 and CD59, and weakly receptor C3AR were detected in the investigated FNF and OA cartilage, except for CD46, which was detected in only two of the five investigated donors. The in-house score also allows inexperienced observers to distinguish non-OA and OA cartilage for experimental purposes.

scholarly journals Oncogenic activation of FAK drives apoptosis suppression in a 3D-culture model of breast cancer initiation

Oncotarget ◽  
2016 ◽  
Vol 7 (43) ◽  
pp. 70336-70352 ◽  
Author(s):  
Scott Walker ◽  
Fiona Foster ◽  
Amber Wood ◽  
Thomas Owens ◽  
Keith Brennan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
3D Culture ◽  
Culture Model ◽  
Cancer Initiation ◽  
3D Culture Model
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