Highly sensitive fluorescent detection of glutathione and histidine based on the Cu(ii)-thiamine system

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4442-4447 ◽  
Author(s):  
Pengjuan Ni ◽  
Dafeng Jiang ◽  
Chuanxia Chen ◽  
Yuanyuan Jiang ◽  
Yizhong Lu ◽  
...  

A novel fluorescence method for the simultaneous detection of glutathione and histidine based on their inhibitory effects on the oxidation of thiamine by Cu(ii) is proposed.

Nanoscale ◽  
2019 ◽  
Vol 11 (17) ◽  
pp. 8119-8123 ◽  
Author(s):  
Yiting Jiang ◽  
Yuguo Tang ◽  
Peng Miao

An innovative fluorescence method is developed for simultaneous detection of multiplex tumor markers based on a polydopamine nanosphere@silver nanocluster system.


The Analyst ◽  
2021 ◽  
Vol 146 (20) ◽  
pp. 6297-6305
Author(s):  
Qinglan Miao ◽  
Ji Qi ◽  
Yuanyuan Li ◽  
Xinxia Fan ◽  
Dongmei Deng ◽  
...  

A novel paper-based chip that anchored zinc-doped carbon dots was constructed for sensitive and stable fluorescent detection of Cu2+. Zn doping increased the active sites for simplifying the modification of carbon dots.


Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1207
Author(s):  
Hong Jae Cheon ◽  
Quynh Huong Nguyen ◽  
Moon Il Kim

Inspired by the active site structure of natural horseradish peroxidase having iron as a pivotal element with coordinated histidine residues, we have developed histidine coated magnetic nanoparticles (His@MNPs) with relatively uniform and small sizes (less than 10 nm) through one-pot heat treatment. In comparison to pristine MNPs and other amino acid coated MNPs, His@MNPs exhibited a considerably enhanced peroxidase-imitating activity, approaching 10-fold higher in catalytic reactions. With the high activity, His@MNPs then were exploited to detect the important neurotransmitter acetylcholine. By coupling choline oxidase and acetylcholine esterase with His@MNPs as peroxidase mimics, target choline and acetylcholine were successfully detected via fluorescent mode with high specificity and sensitivity with the limits of detection down to 200 and 100 nM, respectively. The diagnostic capability of the method is demonstrated by analyzing acetylcholine in human blood serum. This study thus demonstrates the potential of utilizing His@MNPs as peroxidase-mimicking nanozymes for detecting important biological and clinical targets with high sensitivity and reliability.


2013 ◽  
Vol 49 (90) ◽  
pp. 10599 ◽  
Author(s):  
Yan-Xia Qi ◽  
Min Zhang ◽  
Qian-Qian Fu ◽  
Ran Liu ◽  
Guo-Yue Shi

2016 ◽  
Vol 8 (44) ◽  
pp. 7929-7936 ◽  
Author(s):  
Liling Hao ◽  
Huajie Gu ◽  
Nuo Duan ◽  
Shijia Wu ◽  
Zhouping Wang

In this study, a highly sensitive and specific multiplex chemiluminescent method for the simultaneous detection of three antibiotics was fabricated.


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