scholarly journals In vivo detection of drug-induced apoptosis in tumors using Raman spectroscopy

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 4836-4839 ◽  
Author(s):  
Oliver Jonas ◽  
Jeon Woong Kang ◽  
Surya P. Singh ◽  
Alex Lammers ◽  
Freddy T. Nguyen ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Study Drug ◽  
Label Free ◽  
Drug Induced ◽  
In Vivo Detection ◽  
Induced Apoptosis

We describe a label-free approach based on Raman spectroscopy, to study drug-induced apoptosis in vivo.

scholarly journals Applications of Vibrational Spectroscopy for Analysis of Connective Tissues

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 922
Author(s):  
William Querido ◽  
Shital Kandel ◽  
Nancy Pleshko
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Biological Tissues ◽  
Label Free ◽  
Connective Tissues ◽  
Bone Properties ◽  
Composition And Structure

Advances in vibrational spectroscopy have propelled new insights into the molecular composition and structure of biological tissues. In this review, we discuss common modalities and techniques of vibrational spectroscopy, and present key examples to illustrate how they have been applied to enrich the assessment of connective tissues. In particular, we focus on applications of Fourier transform infrared (FTIR), near infrared (NIR) and Raman spectroscopy to assess cartilage and bone properties. We present strengths and limitations of each approach and discuss how the combination of spectrometers with microscopes (hyperspectral imaging) and fiber optic probes have greatly advanced their biomedical applications. We show how these modalities may be used to evaluate virtually any type of sample (ex vivo, in situ or in vivo) and how “spectral fingerprints” can be interpreted to quantify outcomes related to tissue composition and quality. We highlight the unparalleled advantage of vibrational spectroscopy as a label-free and often nondestructive approach to assess properties of the extracellular matrix (ECM) associated with normal, developing, aging, pathological and treated tissues. We believe this review will assist readers not only in better understanding applications of FTIR, NIR and Raman spectroscopy, but also in implementing these approaches for their own research projects.

Early, in vivo, Detection of Abiotic Plant Stress Responses via Raman Spectroscopy

2016 ◽  
Author(s):  
Narangerel Altangerel ◽  
Gombojav O. Ariunbold ◽  
Connor Gorman ◽  
Dwight Bohlmeyer ◽  
Joshua Yuan ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Stress Responses ◽  
Plant Stress ◽  
In Vivo Detection ◽  
Plant Stress Responses

In vitro susceptibility to dexamethasone- and doxorubicin-induced apoptotic cell death in context of maturation stage, responsiveness to interleukin 7, and early cytoreduction in vivo in childhood T-cell acute lymphoblastic leukemia

Blood ◽  
2002 ◽  
Vol 99 (11) ◽  
pp. 4109-4115 ◽  
Author(s):  
Christian Wuchter ◽  
Velia Ruppert ◽  
Martin Schrappe ◽  
Bernd Dörken ◽  
Wolf-Dieter Ludwig ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Apoptotic Cell ◽  
Lymphoblastic Leukemia ◽  
Therapy Response ◽  
Drug Induced ◽  
In Vitro Susceptibility ◽  
Interleukin 7 ◽  
Induced Apoptosis

Within childhood T-cell acute lymphoblastic leukemia (T-ALL), patients with a cortical (CD1a+) immunophenotype have been identified as a subgroup with favorable outcome in the acute lymphoblastic leukemia–Berlin-Frankfurt-Münster (ALL-BFM), Cooperative study group for childhood acute lymphoblastic leukemia (COALL) and Pediatric Oncology Group studies. We investigated in leukemic samples of children with T-ALL (n = 81) whether the different in vivo therapy response could be linked to differential in vitro susceptibility to apoptotic cell death. The extent of dexamethasone- as well as doxorubicin-induced apoptosis, detected by annexin V staining, positively correlated with the expression levels of CD1a (Spearman correlation coefficient, rs = 0.3 and 0.4, respectively; P < .01). When compared to cortical T-ALL, mature (CD1a− , surface CD3+) T-ALL were significantly more resistant to doxorubicin, and immature, pro–/pre–T-ALL were more resistant to both drugs (P < .05). Apoptosis-related parameters (Bax, Bcl-2, CD95, and CD95-induced apoptosis) did not account for differential susceptibility to drug-induced apoptosis. By contrast, an interleukin 7–induced rescue of leukemic cells from spontaneous apoptosis, recently proposed to reflect distinct developmental stages and apoptotic programs in T-ALL, was highly associated with susceptibility to dexamethasone- but not doxorubicin-induced apoptosis (P < .001 versus P = .08). Analysis of clinical data showed that in vitro susceptibility to dexamethasone (but not to doxorubicin) closely correlated with early in vivo therapy response characterized by percentages of blast cells in bone marrow on day 15 (rs = −0.46, P = .001). Taken together, the in vitro assessment of drug-induced apoptosis revealed maturation-dependent differences within childhood T-ALL. The enhanced sensitivity to both drugs in cortical T-ALL might account for the better in vivo treatment response of this prognostically favorable T-ALL subgroup.

scholarly journals Drug-induced Apoptosis and p53, BCL-2 and BAX Expression in Breast Cancer Tissues In Vivo and in Fibroblast Cells In Vitro

1999 ◽  
Vol 29 (7) ◽  
pp. 323-331 ◽  
Author(s):  
K. Suzuki ◽  
T. Kazui ◽  
M. Yoshida ◽  
T. Uno ◽  
T. Kobayashi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fibroblast Cells ◽  
Drug Induced ◽  
Induced Apoptosis ◽  
Cancer Tissues

High Wavenumber Raman Spectroscopy for in Vivo Detection of Cervical Dysplasia

2009 ◽  
Vol 81 (21) ◽  
pp. 8908-8915 ◽  
Author(s):  
Jianhua Mo ◽  
Wei Zheng ◽  
Jeffrey J. H. Low ◽  
Joseph Ng ◽  
A. Ilancheran ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cervical Dysplasia ◽  
In Vivo Detection

Image-guided near-infrared Raman spectroscopy for in vivo detection of gastric dysplasia

2009 ◽  
Author(s):  
Zhiwei Huang ◽  
Seng Khoon Teh ◽  
Wei Zheng ◽  
Khek Yu Ho ◽  
Ming Teh ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Near Infrared ◽  
Image Guided ◽  
Gastric Dysplasia ◽  
In Vivo Detection
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