A new enzyme immunoassay for alpha-fetoprotein in a separate setup coupling an aluminium/Prussian blue-based self-powered electrochromic display with a digital multimeter readout

The Analyst ◽  
2018 ◽  
Vol 143 (13) ◽  
pp. 2992-2996 ◽  
Author(s):  
Zhenzhong Yu ◽  
Guoneng Cai ◽  
Rongrong Ren ◽  
Dianping Tang
Keyword(s):  
Prussian Blue ◽  
Enzyme Immunoassay ◽  
Alpha Fetoprotein ◽  
Model Coupling ◽  
Self Powered

A new immunoassay was designed for the detection of disease-related biomarkers (alpha-fetoprotein, AFP, as a model), coupling an aluminium (Al)/Prussian blue-based electrochromic display with a digital multimeter readout.

Sensitive sandwich electrochemical immunosensor for alpha fetoprotein based on prussian blue modified hydroxyapatite

2011 ◽  
Vol 28 (1) ◽  
pp. 112-116 ◽  
Author(s):  
Yuxue Dai ◽  
Yanyan Cai ◽  
Yanfang Zhao ◽  
Dan Wu ◽  
Bao Liu ◽  
...  
Keyword(s):  
Prussian Blue ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein

Heterogeneous enzyme immunoassay of alpha-fetoprotein in maternal serum by flow-injection amperometric detection of 4-aminophenol

1990 ◽  
Vol 36 (11) ◽  
pp. 1941-1944 ◽  
Author(s):  
Y Xu ◽  
B Halsall ◽  
W R Heineman
Keyword(s):  
Enzyme Immunoassay ◽  
Flow Injection ◽  
Enzymatic Reaction ◽  
Amperometric Detection ◽  
Maternal Serum ◽  
Alpha Fetoprotein ◽  
Serum Samples ◽  
Low Concentrations ◽  
Sandwich Type ◽  
Commercial Kit

Abstract A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was less than 8.2%, with a minimum detection limit for AFP of 0.163 micrograms/L. The calibration curve had a linear range of 0.316-100 micrograms/L. Studies with 48 human maternal serum samples, comparing results by this method with those by a commercial kit, showed a good correlation (r = 0.961). This procedure provides an alternative method for determining low concentrations of AFP in human maternal serum.

Biofunctionalized dendritic polyaniline nanofiber for in situ amplified glucometer-based enzyme immunoassay of tumor marker

2015 ◽  
Vol 7 (5) ◽  
pp. 1843-1848 ◽  
Author(s):  
Sheng Wu ◽  
Jinsuo Chen ◽  
Yujian Tian ◽  
Xiaoming Tang ◽  
Wei Li ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Tumor Marker ◽  
Enzyme Immunoassay ◽  
Alpha Fetoprotein ◽  
Sensitive Detection ◽  
Polyaniline Nanofibers ◽  
Polyaniline Nanofiber

A new glucometer-based enzyme immunoassay was developed for the sensitive detection of alpha-fetoprotein using biofunctional dendritic polyaniline nanofibers as the signal-transduction tag.

Chemiluminescent enzyme immunoassay of alpha-fetoprotein based on an adamantyl dioxetane phenyl phosphate substrate.

1989 ◽  
Vol 35 (12) ◽  
pp. 2319-2321 ◽  
Author(s):  
G H Thorpe ◽  
I Bronstein ◽  
L J Kricka ◽  
B Edwards ◽  
J C Voyta
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Immunoassay ◽  
Detection Limits ◽  
Alpha Fetoprotein ◽  
Phenyl Phosphate ◽  
Comparison Of Results ◽  
Enzyme Detection ◽  
Chemiluminescent Enzyme Immunoassay

Abstract We have evaluated a new chemiluminescent substrate for the alkaline phosphatase (EC 3.1.3.1) label used in a Hybritech Tandem-E immunoassay of alpha-fetoprotein (AFP). The new substrate, adamantyl 1,2-dioxetane phenyl phosphate (AMPPD), emits light at 477 nm when acted upon by the enzyme. Detection limits for AFP with this method were 33 ng/L (mean of 20 replicates of the zero standard + 2 SD) and 470 ng/L (twice background). Between-batch CVs ranged from 4.31% to 9.60% for AFP in the range 29.1-132.0 micrograms/L. Comparison of results for 49 specimens assayed with use of the chemiluminescent kit and a colorimetric version of the AFP assay gave statistical values as follows: slope = 0.88, intercept = 4.19, and r = 0.94.

Sign in / Sign up

Export Citation Format

Share Document