scholarly journals Development of highly sensitive fluorescent probes for the detection of β-galactosidase activity – application to the real-time monitoring of senescence in live cells

The Analyst ◽  
2018 ◽  
Vol 143 (11) ◽  
pp. 2680-2688 ◽  
Author(s):  
Mauro Safir Filho ◽  
Pascal Dao ◽  
Maéva Gesson ◽  
Anthony R. Martin ◽  
Rachid Benhida
Keyword(s):  
Real Time ◽  
Fluorescent Probes ◽  
Live Cells ◽  
Galactosidase Activity ◽  
Real Time Monitoring ◽  
The Real ◽  
Fluorescent Reporters ◽  
Highly Sensitive

Highly sensitive fluorescent reporters for the detection and monitoring of β-galactosidase activity in live cells.

A supersensitive biosensor based on MoS2 nanosheet arrays for the real-time detection of H2O2 secreted from living cells

2019 ◽  
Vol 55 (65) ◽  
pp. 9653-9656 ◽  
Author(s):  
Huitong Du ◽  
Xinyue Zhang ◽  
Zhe Liu ◽  
Fengli Qu
Keyword(s):  
Real Time ◽  
Living Cells ◽  
Sensitive Detection ◽  
Live Cells ◽  
The Real ◽  
Highly Sensitive ◽  
Nanosheet Arrays ◽  
Mos2 Nanosheet ◽  
Real Time Detection ◽  
Highly Sensitive Detection

A self-supported MoS2 nanosheet biosensor for highly sensitive detection of H2O2 secreted from live cells.

Cr doped WO3 nanofibers enriched with surface oxygen vacancies for highly sensitive detection of the 3-hydroxy-2-butanone biomarker

2018 ◽  
Vol 6 (43) ◽  
pp. 21419-21427 ◽  
Author(s):  
Zhengyou Zhu ◽  
Lijun Zheng ◽  
Shizheng Zheng ◽  
Jian Chen ◽  
Minghui Liang ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Real Time ◽  
Oxygen Vacancies ◽  
Sensitive Detection ◽  
Surface Oxygen ◽  
Real Time Monitoring ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Cr doped WO3 nanofibers enriched with surface oxygen vacancies have been developed to smartly detect the 3H-2B biomarker for the real-time monitoring of Listeria monocytogenes.

Synthesis of aqueous AgInS/ZnS@PEI as a self-indicating nonviral vector for plasmid DNA self-tracking delivery

2015 ◽  
Vol 3 (43) ◽  
pp. 8518-8527 ◽  
Author(s):  
Wentao Yang ◽  
Weisheng Guo ◽  
Tingbin Zhang ◽  
Weitao Yang ◽  
Lin Su ◽  
...  
Keyword(s):  
Real Time ◽  
Plasmid Dna ◽  
Gene Transfection ◽  
Fluorescence Labeling ◽  
Live Cells ◽  
Nonviral Vector ◽  
Real Time Monitoring ◽  
The Real ◽  
Gene Vectors

Synthesis of aqueous ZAIS@PEI QDs as versatile self-tracking gene vectors, allowing the real-time monitoring of gene transfection behavior in live cells without external fluorescence labeling.

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

Observing the real time formation of phosphine-ligated gold clusters by electrospray ionization mass spectrometry

2017 ◽  
Vol 19 (26) ◽  
pp. 17187-17198 ◽  
Author(s):  
Marshall R. Ligare ◽  
Grant E. Johnson ◽  
Julia Laskin
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Real Time ◽  
Electrospray Ionization Mass Spectrometry ◽  
Gold Cluster ◽  
Gold Clusters ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Real Time Monitoring ◽  
The Real

Real-time monitoring of the gold cluster synthesis by electrospray ionization mass spectrometry reveals distinct formation pathways for Au8, Au9 and Au10 clusters.

A ratiometric fluorogenic probe for the real-time detection of SO32−in aqueous medium: application in a cellulose paper based device and potential to sense SO32−in mitochondria

The Analyst ◽  
2018 ◽  
Vol 143 (1) ◽  
pp. 250-257 ◽  
Author(s):  
Soham Samanta ◽  
Senjuti Halder ◽  
Poulomi Dey ◽  
Utsab Manna ◽  
Aiyagari Ramesh ◽  
...  
Keyword(s):  
Aqueous Medium ◽  
Real Time ◽  
Water Soluble ◽  
Live Cells ◽  
The Real ◽  
Cellulose Paper ◽  
Fluorogenic Probe ◽  
Ratiometric Detection ◽  
Real Time Detection

A new water soluble and fluorogenic probe (L) that can demonstrate the specific ratiometric detection of a SO2derivative (SO32−) in 100% aqueous medium and live cells has been designed and synthesized.

A Real-Time PCR Assay for the Detection of Salmonella in a Wide Variety of Food and Food-Animal Matrices†

2007 ◽  
Vol 70 (5) ◽  
pp. 1080-1087 ◽  
Author(s):  
V. M. BOHAYCHUK ◽  
G. E. GENSLER ◽  
M. E. McFALL ◽  
R. K. KING ◽  
D. G. RENTER
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Culture Method ◽  
Pcr Assay ◽  
Culture Methods ◽  
The Real ◽  
Conventional Culture ◽  
Food Animal ◽  
Highly Sensitive ◽  
Field Samples

Conventional culture methods have traditionally been considered the “gold standards” for the isolation and identification of foodborne pathogens. However, culture methods are labor-intensive and time-consuming. We have developed a real-time PCR assay for the detection of Salmonella in a variety of food and food-animal matrices. The real-time PCR assay incorporates both primers and hybridization probes based on the sequence of the Salmonella invA gene and uses fluorescent resonance energy transfer technology to ensure highly sensitive and specific results. This method correctly classified 51 laboratory isolates of Salmonella and 28 non-Salmonella strains. The method was also validated with a large number of field samples that consisted of porcine feces and cecal contents, pork carcasses, bovine feces and beef carcasses, poultry cecal contents and carcasses, equine feces, animal feeds, and various food products. The samples (3,388) were preenriched in buffered peptone water and then selectively enriched in tetrathionate and Rappaport-Vassiliadis broths. Aliquots of the selective enrichment broths were combined for DNA extraction and analysis by the real-time PCR assay. When compared with the culture method, the diagnostic sensitivity of the PCR assay for the various matrices ranged from 97.1 to 100.0%, and the diagnostic specificity ranged from 91.3 to 100.0%. Kappa values ranged from 0.87 to 1.00, indicating excellent agreement of the real-time PCR assay to the culture method. The reduction in time and labor makes this highly sensitive and specific real-time PCR assay an excellent alternative to conventional culture methods for surveillance and research studies to improve food safety.

Sign in / Sign up

Export Citation Format

Share Document