Raman imaging of heme metabolism in situ in macrophages and Kupffer cells

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3489-3498 ◽  
Author(s):  
J. Dybas ◽  
M. Grosicki ◽  
M. Baranska ◽  
K. M. Marzec
Keyword(s):  
Cell Line ◽  
Kupffer Cells ◽  
Blood Cells ◽  
Raman Imaging ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Imaging Results

Herein, we provide the Raman imaging results for different stages of erythrophagocytosis of senescent red blood cells executed by isolated murine primary Kupffer cells and a murine macrophage cell line (RAW 264.7).

scholarly journals Evaluation of Methods for Transient Transfection of a Murine Macrophage Cell Line, RAW 264.7

BioTechniques ◽  
1999 ◽  
Vol 27 (4) ◽  
pp. 824-832 ◽  
Author(s):  
C.D. Thompson ◽  
M.R. Frazier-Jessen ◽  
R. Rawat ◽  
R.P. Nordan ◽  
R.T. Brown
Keyword(s):  
Cell Line ◽  
Transient Transfection ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Evaluation Of Methods

Activation of murine macrophage cell line RAW 264.7 by Korean propolis

2002 ◽  
Vol 25 (6) ◽  
pp. 895-902 ◽  
Author(s):  
Shinha Han ◽  
Ki -Hyun Sung ◽  
Dongsool Yim ◽  
Sookyeon Lee ◽  
Kyunghae Cho ◽  
...  
Keyword(s):  
Cell Line ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Murine Macrophage Cell Line ◽  
Korean Propolis

Ferritin does not donate its iron for haem synthesis in macrophages

2010 ◽  
Vol 429 (3) ◽  
pp. 463-471 ◽  
Author(s):  
Marc Mikhael ◽  
Alex D. Sheftel ◽  
Prem Ponka
Keyword(s):  
Single Cell ◽  
Cell Line ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Iron Storage ◽  
Subunit Protein ◽  
Metabolic Functions

Iron is essential for all life, yet can be dangerous under certain conditions. Iron storage by the 24-subunit protein ferritin renders excess amounts of the metal non-reactive and, consequentially, ferritin is crucial for life. Although the mechanism detailing the storage of iron in ferritin has been well characterized, little is known about the fate of ferritin-stored iron and whether it can be released and reutilized for metabolic use within a single cell. Virtually nothing is known about the use of ferritin-derived iron in non-erythroid cells. We therefore attempted to answer the question of whether iron from ferritin can be used for haem synthesis in the murine macrophage cell line RAW 264.7 cells. Cells treated with ALA (5-aminolaevulinic acid; a precursor of haem synthesis) show increased haem production as determined by enhanced incorporation of transferrin-bound 59Fe into haem. However, the present study shows that, upon the addition of ALA, 59Fe from ferritin cannot be incorporated into haem. Additionally, little 59Fe is liberated from ferritin when haem synthesis is increased upon addition of ALA. In conclusion, ferritin in cultivated macrophages is not a significant source of iron for the cell's own metabolic functions.

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