Diversity among endothelial cell lines revealed by Raman and Fourier-transform infrared spectroscopic imaging

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4323-4334 ◽  
Author(s):  
Ewelina Szafraniec ◽  
Ewelina Wiercigroch ◽  
Krzysztof Czamara ◽  
Katarzyna Majzner ◽  
Emilia Staniszewska-Slezak ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Fourier Transform ◽  
Endothelial Cell ◽  
Cell Lines ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Human Endothelial Cells ◽  
Infrared Spectroscopic ◽  
Infrared Spectroscopic Imaging

A methodology of examination and characterization of popular human endothelial cells lines.

Time-Resolved Fourier Transform Infrared Spectroscopic Imaging

2003 ◽  
Vol 57 (4) ◽  
pp. 357-366 ◽  
Author(s):  
Rohit Bhargava ◽  
Ira W. Levin
Keyword(s):  
Fourier Transform ◽  
Imaging Modality ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Dynamic Processes ◽  
Time Resolved ◽  
Ir Imaging ◽  
Infrared Spectroscopic ◽  
Infrared Spectroscopic Imaging ◽  
Ft Ir

Fourier transform infrared (FT-IR) imaging allows simultaneous spectral characterization of large spatial areas due to its multichannel detection advantage. The acquisition of large amounts of data in the multichannel configuration results, however, in a poor temporal resolution of sequentially acquired data sets, which limits the examination of dynamic processes to processes that have characteristic time scales of the order of minutes. Here, we introduce the concept and instrumental details of a time-resolved infrared spectroscopic imaging modality that permits the examination of repetitive dynamic processes whose half-lives are of the order of milliseconds. As an illustration of this implementation of step-scan FT-IR imaging, we examine the molecular responses to external electric-field perturbations of a microscopically heterogeneous polymer–liquid crystal composite. Analysis of the spectroscopic data using conventional univariate and generalized two-dimensional (2D) correlation methods emphasizes an additional capability for accessing of simultaneous spatial and temporal chemical measurements of molecular dynamic processes.

Novel Route to Faster Fourier Transform Infrared Spectroscopic Imaging

2001 ◽  
Vol 55 (8) ◽  
pp. 1079-1084 ◽  
Author(s):  
Rohit Bhargava ◽  
Michael D. Schaeberle ◽  
Daniel C. Fernandez ◽  
Ira W. Levin
Keyword(s):  
Fourier Transform ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Infrared Spectroscopic ◽  
Infrared Spectroscopic Imaging

scholarly journals Fourier Transform Infrared Spectroscopic Imaging of Fracture Healing in the Normal Mouse

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Hans Gollwitzer ◽  
Xu Yang ◽  
Lyudmila Spevak ◽  
Lyudmila Lukashova ◽  
Allina Nocon ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Fracture Healing ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Therapeutic Interventions ◽  
Bone Collagen ◽  
Endochondral Bone Formation ◽  
Mineral Density ◽  
Infrared Spectroscopic ◽  
Infrared Spectroscopic Imaging

Fourier transform infrared spectroscopic imaging (FTIRI) was used to study bone healing with spatial analysis of various callus tissues in wild type mice. Femoral fractures were produced in 28 male C57BL mice by osteotomy. Animals were sacrificed at 1, 2, 4, and 8 weeks to obtain callus tissue at well-defined healing stages. Following microcomputerized tomography, bone samples were cut in consecutive sections for FTIRI and histology, allowing for spatial correlation of both imaging methods in different callus areas (early calcified cartilage, woven bone, areas of intramembranous and endochondral bone formation). Based on FTIRI, mineral/matrix ratio increased significantly during the first 4 weeks of fracture healing in all callus areas and correlated with bone mineral density measured by micro-CT. Carbonate/phosphate ratio was elevated in newly formed calcified tissue and at week 2 attained values comparable to cortical bone. Collagen maturity and mineral crystallinity increased during weeks 1–8 in most tissues while acid phosphate substitution decreased. Temporal and callus area dependent changes were detected throughout the healing period. These data assert the usefulness of FTIRI for evaluation of fracture healing in the mouse and its potential to evaluate pathologic fracture healing and the effects of therapeutic interventions.

High Throughput Fourier Transform Infrared Spectroscopic Imaging for Histopathology

2004 ◽  
Vol 10 (S02) ◽  
pp. 1302-1303
Author(s):  
Rohit Bhargava ◽  
D C Fernandez ◽  
S M Hewitt ◽  
Ira W Levin
Keyword(s):  
Fourier Transform ◽  
High Throughput ◽  
Spectroscopic Imaging ◽  
Fourier Transform Infrared ◽  
Infrared Spectroscopic ◽  
Infrared Spectroscopic Imaging

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.

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