An improved detection limit and working range of lateral flow assays based on a mathematical model

The Analyst ◽  
2018 ◽  
Vol 143 (12) ◽  
pp. 2775-2783 ◽  
Author(s):  
Zhi Liu ◽  
Zhiguo Qu ◽  
Ruihua Tang ◽  
Xiaocong He ◽  
Hui Yang ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Detection Limit ◽  
Lateral Flow ◽  
Lateral Flow Assays

The detection limit and working range of lateral flow assays are investigated experimentally and numerically.

scholarly journals Two Orders of Magnitude Improvement in Detection Limit of Lateral Flow Assays Using Isotachophoresis

2015 ◽  
Vol 87 (2) ◽  
pp. 1009-1017 ◽  
Author(s):  
Babak Y. Moghadam ◽  
Kelly T. Connelly ◽  
Jonathan D. Posner
Keyword(s):  
Detection Limit ◽  
Lateral Flow ◽  
Lateral Flow Assays ◽  
Magnitude Improvement

A two-dimensional mathematical model for analyzing the effects of capture probe properties on the performance of lateral flow assays

The Analyst ◽  
2019 ◽  
Vol 144 (18) ◽  
pp. 5394-5403
Author(s):  
Zhi Liu ◽  
Xiaocong He ◽  
Ang Li ◽  
Zhiguo Qu ◽  
Feng Xu
Keyword(s):  
Mathematical Model ◽  
Low Cost ◽  
Lateral Flow ◽  
Capture Probe ◽  
Two Dimensional ◽  
Biomedical Diagnosis ◽  
Lateral Flow Assays

Lateral flow assays (LFAs) are promising candidates in biomedical diagnosis fields due to their rapid, low-cost, and portable features.

The effect of report particle properties on lateral flow assays: A mathematical model

2017 ◽  
Vol 248 ◽  
pp. 699-707 ◽  
Author(s):  
Zhi Liu ◽  
Jie Hu ◽  
Ang Li ◽  
Shangsheng Feng ◽  
Zhiguo Qu ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Lateral Flow ◽  
Particle Properties ◽  
Lateral Flow Assays

scholarly journals Comparative Study of Four Coloured Nanoparticle Labels in Lateral Flow Immunoassay

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3277
Author(s):  
Shyatesa C. Razo ◽  
Anastasiya I. Elovenkova ◽  
Irina V. Safenkova ◽  
Natalia V. Drenova ◽  
Yuri A. Varitsev ◽  
...  
Keyword(s):  
Detection Limit ◽  
Physical Adsorption ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Shell Nanoparticles ◽  
Au Nps ◽  
Bacterial Agent ◽  
Functional Coverage ◽  
Lateral Flow Assays

The detection limit of lateral flow immunoassay (LFIA) is largely determined by the properties of the label used. We compared four nanoparticle labels differing in their chemical composition and colour: (1) gold nanoparticles (Au NPs), red; (2) Au-core/Pt-shell nanoparticles (Au@Pt NPs), black; (3) latex nanoparticles (LPs), green; and (4) magnetic nanoparticles (MPs), brown. The comparison was carried out using one target analyte—Erwinia amylovora, the causal bacterial agent of fire blight. All nanoparticles were conjugated with antibodies through methods that provide maximum functional coverage like physical adsorption (Au NPs, Au@Pt NPs) and covalent bonding (LPs, MPs). All conjugates demonstrated the same ability to bind with E. amylovora through enzyme-linked immunosorbent assay where optical properties of the nanoparticles do not determine the registered signal. However, half-maximal binding was achieved at different numbers of nanoparticles because they differ in size. All conjugates based on four nanoparticle labels were used for lateral flow assays. As a result, Au@Pt NPs provided the minimal detection limit that corresponded to 103 CFU/mL. Au NPs and LPs detected 104 CFU/mL, and MPs detected 105 CFU/mL. The results highlight that simply choosing a coloured label can significantly affect the detection limit of LFIA.

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

Inductively coupled plasma optical emission spectroscopy as a tool for evaluating lateral flow assays

2021 ◽  
Author(s):  
Jenna M. DeSousa ◽  
Micaella Z. Jorge ◽  
Hayley B. Lindsay ◽  
Frederick R. Haselton ◽  
David W. Wright ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Emission Spectroscopy ◽  
Optical Emission Spectroscopy ◽  
Optical Emission ◽  
Lateral Flow ◽  
Gold Content ◽  
Icp Oes ◽  
Inductively Coupled ◽  
Lateral Flow Assays ◽  
Plasma Optical Emission Spectroscopy

This work demonstrates the first use of ICP-OES to quantitatively analyze gold content on lateral flow assays.

scholarly journals Integrating high-performing electrochemical transducers in lateral flow assay

2021 ◽  
Author(s):  
Antonia Perju ◽  
Nongnoot Wongkaew
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Analytical Performance ◽  
Label Free ◽  
High Performing ◽  
Lateral Flow Assays ◽  
Electrochemical Transducers

AbstractLateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform. Commercially, visual and optical detection strategies dominate, but it is especially the research on integrating electrochemical (EC) approaches that may have a chance to significantly improve an LFA’s performance that is needed in order to detect analytes reliably at lower concentrations than currently possible. In fact, EC-LFAs offer advantages in terms of quantitative determination, low-cost, high sensitivity, and even simple, label-free strategies. Here, the various configurations of EC-LFAs published are summarized and critically evaluated. In short, most of them rely on applying conventional transducers, e.g., screen-printed electrode, to ensure reliability of the assay, and additional advances are afforded by the beneficial features of nanomaterials. It is predicted that these will be further implemented in EC-LFAs as high-performance transducers. Considering the low cost of point-of-care devices, it becomes even more important to also identify strategies that efficiently integrate nanomaterials into EC-LFAs in a high-throughput manner while maintaining their favorable analytical performance.

Sign in / Sign up

Export Citation Format

Share Document