Design of a wearable device for real-time screening of urinary tract infection and kidney disease based on smartphone

The Analyst ◽  
2018 ◽  
Vol 143 (12) ◽  
pp. 2812-2818 ◽  
Author(s):  
Jianyu Zhou ◽  
Tao Dong
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Quantitative Analysis ◽  
Kidney Disease ◽  
Tract Infection ◽  
Real Time ◽  
Low Cost ◽  
Wearable Device ◽  
Urine Samples

In this study, we developed a novel wearable and low-cost device for qualitative screening of glucose (GLU), leukocytes (LEU), and nitrite (NIT) and for semi-quantitative analysis of blood (BLD) and proteins (PRO) in the urine samples.

scholarly journals Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection

2015 ◽  
Vol 2 (4) ◽  
pp. 31990-31990 ◽  
Author(s):  
Fariha Akhter Chowdhury ◽  
Mohammad Nurul Islam ◽  
Anamika Saha ◽  
Sabrina Mahboob ◽  
Abu Syed Md. Mosaddek ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urine Samples

scholarly journals Urinary Tract Infection in Children: A Review of the Established Practice Guidelines

2020 ◽  
pp. 57-65
Author(s):  
Samuel Uwaezuoke ◽  
Adaeze Ayuk ◽  
Uzoamaka Muoneke
Keyword(s):  
Chronic Kidney Disease ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Kidney Disease ◽  
Tract Infection ◽  
Practice Guidelines ◽  
Renal Scarring ◽  
Clinical Excellence ◽  
Radiological Investigation ◽  
Delayed Treatment

Urinary tract infection (UTI) is a significant cause of morbidity in children. Delayed treatment is associated with complications that may result in chronic kidney disease and, subsequently, end-stage kidney disease. Over the years, clinical practice guidelines have advanced to ensure the best global practices in treating the infection and preventing its progression to chronic kidney disease. The established practice guidelines address five main questions: 1) which children should have their urine tested; 2) how the sample should be obtained; 3) which radiological tests are recommended after a diagnosis of UTI; 4) how the infection should be treated; 5) and how affected children should be followed up. There is a substantial overlap in the recommendations of the American Academy of Pediatrics (AAP) guidelines and the UK’s National Institute for Health and Clinical Excellence (NICE) guidelines. Subtle differences, however, exist between the two established guidelines. An evidence-based paradigm shift of some traditional concepts about UTI in children has contributed to the revision and update of these guidelines. Further research is needed to clarify the role of host and genetic factors in renal scarring, as well as the diagnostic criteria for UTI. This narrative review aims to discuss the current recommendations of these established practice guidelines with an emphasis on the diagnosis, radiological investigation, treatment, and follow-up of UTI in children.

scholarly journals Multidrug Resistance of Uropathogens at Governmental Hospitals in the Gaza Strip/Palestine

10.3823/855 ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sameh A. Alkhodari ◽  
Abdelraouf A. Elmanama
Keyword(s):  
Urinary Tract Infection ◽  
Multidrug Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Gaza Strip ◽  
Public Health Problem ◽  
Urine Samples ◽  
E Coli ◽  
The Gaza Strip ◽  
Klebsiella Spp

Urinary tract infection is a public health problem worldwide. E. coli and klebsiella are among the main etiologic for UTI in Gaza Strip. The growing variations in resistance among uropathogens to antimicrobials is multifactorial and varies globally. It greatly reduces/limits or complicate treatment option. Aims: To determine the pattern of antimicrobial resistance and multidrug resistance among uropathogens at governmental hospitals. Methods: We analyzed the data of 11,890 urine samples processed in governmental hospitals in the Gaza Strip, Palestine during 2019. The percentage of resistance was calculated for uropathogens, and then multidrug resistance was calculated according to “CDC” definition. Results: Of 11,890 urine samples, 2910 (24.5%) showed significant growth.  Escherichia coli was isolated most frequently (1743; 59.9%), followed by Klebsiella spp. (725; 24.9%), Pseudomonas spp. (123; 4.2%), Streptococcus spp. (98; 3.4%), Staphylococcus aureus (41; 1.4%). Microorganisms resistance was high against Ampicillin (92.4%) and Amoxicillin (91.1%), Co-Trimoxazole (68.2%), Cefalexin (64.9%), Doxycycline (61.9%), Nalidixic acid (53.6%), Cefuroxime (53.0%), Ceftriaxone (48.9%), Ceftazidime (43.1%), Ciprofloxacin (36.9%), Gentamicin (25.8%), Amikacin (3.2%). The resistance of microorganisms in males is higher than females. Multidrug resistance was detected in 37% of E. coli and 53% in Klebsiella spp. Conclusion: Resistance is high and variable among uropathogens isolated from patients in Gaza strip. Both age and gender are risk factors in both infection and resistance pattern. The multidrug resistance percentage is growing remarkably in Gaza Strip. Keywords: Uropathogens, Resistance, Urinary tract infection, Multidrug resistance, Gaza strip, Palestine  

scholarly journals Evaluation of BacterioScan 216Dx in Comparison to Urinalysis as a Screening Tool for Diagnosis of Urinary Tract Infections in Children

2019 ◽  
Vol 57 (9) ◽  
Author(s):  
Ferdaus Hassan ◽  
Heather Bushnell ◽  
Connie Taggart ◽  
Caitlin Gibbs ◽  
Steve Hiraki ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Predictive Value ◽  
Screening Tool ◽  
Urine Culture ◽  
Antibiotic Use ◽  
Urine Samples ◽  
Maldi Tof ◽  
Tract Infections

ABSTRACTUrinalysis (UA) has routinely been used as a screening tool prior to urine culture set up. BacterioScan 216Dx is an FDA-cleared semiautomated system to detect bacterial growth in urine. The aim of this study was to evaluate 216Dx in comparison to UA for diagnosis of urinary tract infection (UTI) in children. Clean-catch, unpreserved urine samples from children aged <18 years were tested by 216Dx, and positive urine samples in media were processed for direct bacterial identification by matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry. Sensitivity and specificity of 216Dx and urinalysis (UA) were determined against urine culture. Of 287 urine samples obtained from children (median age, 108 months), 44.0% and 56.0% were UA positive and negative, respectively, while 216Dx detected 27% and 73% as positive and negative, respectively. Compared to culture, the overall sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 216Dx versus UA were 92.1% versus 97.3%, 82.7% versus 63.8%, 44.8% versus 29.1%, and 98.6% versus 99.3%, respectively. Among 216Dx true-positive (TP) samples (n= 35), 77.0% were successfully identified directly from broth by MALDI-TOF. Among urine samples that were identified as contaminated by culture (n= 127; 44%), the 216Dx detected 93 (73.0%) as negative while UA detected 69 (54.0%) as negative. Although the sensitivities of 216Dx and UA are comparable, the specificity of 216Dx was higher than that of UA. The 216Dx can be used as an alternative/adjunct screening tool to UA to rule out urinary tract infection (UTI) in children. Compared to culture, the faster turnaround time (3 hours) of 216Dx has the potential to reduce unnecessary antibiotic use and improve patient management.

scholarly journals PSEUDOMONAS SPECIES CAUSING URINARY TRACT INFECTION AND ITS ANTIBIOGRAM AT A TERTIARY CARE HOSPITAL

2017 ◽  
Vol 10 (11) ◽  
pp. 50
Author(s):  
Shobha Kl ◽  
Ramachandra L ◽  
Amita Shobha Rao ◽  
Anand Km ◽  
Gowrish Rao S
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Urine Samples ◽  
Automated System ◽  
Care Hospital ◽  
Pseudomonas Species ◽  
Significant Bacteriuria

  Objectives: Pseudomonas species cause urinary tract infection (UTI). This study was conducted to isolate Pseudomonas species causing significant bacteriuria and to analyze its antibiogram at a tertiary care hospital.Methods: About 107 urine samples received in the laboratory from August 2015 to July 2016 having significant bacteriuria with Pseudomonas species were tested for antimicrobial activity. Samples were from both inpatients and patients attending as outpatients to a tertiary care hospital with different age groups and gender. Midstream urine samples were inoculated using a standard calibrated loop into blood agar and MacConkey agar following semi quantitative technique using Kass concept. MALD (Biomerio, ElToile, France) was used for speciation, and Vitex automated system (Biomerio, ElToile, France) was used for antibacterial sensitivity testing.Results: About 107 urine samples contained Pseudomonas species by MALDI obtained from 69 male (64.48%) and 38 (35.51%) female patients. Inpatients were 90 (84.11%) and 17 (15.88%) outpatient department (OPD) patients. Isolation of Pseudomonas aeruginosa was from 90 samples (84.11%) and Pseudomonas putida in 17 (15.88%). Significant bacteriuria was more in the age group of ˃60 years in both genders. Susceptibility to gentamicin was 52 (48.59%), ceftazidime 56 (52.33%), and imipenem was 58 (54.20%). Organism was multidrug resistant in 49 (45.79%) samples.Conclusion: P. aeruginosa remains the most common uropathogen among Pseudomonas species. Drug of choice for inpatients suffering from UTI by Pseudomonas species can be gentamicin and for patients attending OPD can be ceftazidime. Imipenem can be the reserve drug. Periodic antibiotic review is required for proper treatment of UTI.

scholarly journals INCIDENCES OF CANDIDA ALBICANS AND NON-ALBICANS AMONG CATHETER-ASSOCIATED URINARY TRACT INFECTION PATIENTS OF AKOLA CITY

2017 ◽  
Vol 10 (11) ◽  
pp. 115
Author(s):  
Khandare Ln ◽  
Barate Dl
Keyword(s):  
Urinary Tract Infection ◽  
Candida Albicans ◽  
Urinary Tract ◽  
Tract Infection ◽  
Candida Parapsilosis ◽  
Opportunistic Infections ◽  
Urine Samples ◽  
Age Group ◽  
Candida Spp ◽  
Catheter Urine

Objective: Candida spp. is the third leading cause of catheter-related infections. Candida species is a part of human microflora and it becomes pathogenic when certain conditions are present and cause an opportunistic infections. The present study was undertaken to determine incidences of Candida albicans and non-albicans among catheterized urinary tract infection (UTI) patients of Akola city.Methods: A total 60 catheter urine samples were collected from patient of all the age group and both sex who had indwelling urinary catheter. The collected catheterized urine samples of patients from various hospitals of Akola city were used for isolation using HiCrome Candida differential agar.Results: It was found that highest frequency of isolation of Candida spp. was from age group 61-70 years. The predominance of male candidate was more than female having Candida spp. in catheter-associated UTI (C-UTI). Among the Candida spp. C. albicans (64.81%) was predominant over non-albicans spp. while in non-albicans Candida krusei and Candida glabrata were predominant showing 11.11% incidences. It was followed by Candida tropicalis (9.2%) and Candida parapsilosis (3.7%).Conclusion: The incidences of C. albicans and non-albicans were high among catheter-associated UTI patients.

scholarly journals Urinary tract infection in patients with chronic kidney disease

2014 ◽  
Vol 44 ◽  
pp. 145-149 ◽  
Author(s):  
Chih-Yen HSIAO ◽  
Hsing-Lin LIN ◽  
Yen-Ko LIN ◽  
Chao-Wen CHEN ◽  
Yuan-Chia CHENG ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Kidney Disease ◽  
Tract Infection

scholarly journals Insights into cellular and molecular basis for urinary tract infection in autosomal-dominant polycystic kidney disease

2017 ◽  
Vol 313 (5) ◽  
pp. F1077-F1083 ◽  
Author(s):  
Chao Gao ◽  
Long Zhang ◽  
Ye Zhang ◽  
Darren P. Wallace ◽  
Reynold I. Lopez-Soler ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Kidney Disease ◽  
Tract Infection ◽  
Polycystic Kidney Disease ◽  
Molecular Mechanisms ◽  
Autosomal Dominant ◽  
Immune Defense ◽  
Polycystic Kidney ◽  
Autosomal Dominant Polycystic Kidney

Urinary tract infection (UTI) is a broad term referring to an infection of the kidneys, ureters, bladder, and/or urethra. Because of its prevalence, frequent recurrence, and rising resistance to antibiotics, UTI has become a challenge in clinical practice. Autosomal-dominant polycystic kidney disease (ADPKD) is the most common monogenic disorder of the kidney and is characterized by the growth of fluid-filled cysts in both kidneys. Progressive cystic enlargement, inflammation, and interstitial fibrosis result in nephron loss with subsequent decline in kidney function. ADPKD patients frequently develop UTI; however, the cellular and molecular mechanisms responsible for the high UTI incidence in ADPKD patients remain virtually unaddressed. Emerging evidence suggests that α-intercalated cells (α-ICs) of the collecting ducts function in the innate immune defense against UTI. α-ICs inhibit bacterial growth by acidifying urine and secreting neutrophil gelatinase-associated lipocalin (NGAL) that chelates siderophore-containing iron. It is necessary to determine, therefore, if ADPKD patients with recurrent UTI have a reduced number and/or impaired function of α-ICs. Identification of the underlying cellular and molecular mechanisms may lead to the development of novel strategies to reduce UTI in ADPKD.

scholarly journals Urinary tract infection in patients with chronic kidney disease under conservative treatment

2022 ◽  
Vol 75 (3) ◽  
Author(s):  
Graciana Maria de Moraes Coutinho ◽  
Emanuela Cardoso da Silva ◽  
Cássia Regina Vancini Campanharo ◽  
Angélica Gonçalves Silva Belasco ◽  
Cassiane Dezoti da Fonseca ◽  
...  
Keyword(s):  
Risk Factors ◽  
Chronic Kidney Disease ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Kidney Disease ◽  
Tract Infection ◽  
Conservative Treatment ◽  
University Hospital ◽  
Cross Sectional ◽  
Associated Risk Factors

ABSTRACT Objectives: to assess the prevalence and associated risk factors for urinary tract infection in patients with chronic kidney disease under conservative treatment and identify the microorganisms isolated in the urine of these patients and the staging of chronic kidney disease. Methods: a cross-sectional, analytical study carried out at the Conservative Treatment Outpatient Clinic of a university hospital in the city of São Paulo. Results: the prevalence of urinary tract infection is 22%. The risk factors Diabetes Mellitus, hypertension, heart disease, neoplasms and thyroid and autoimmune diseases stand out in the infected group (p < 0.001). Most of the microorganisms found in urine cultures (87.9%) were Gram-negative, being Escherichia coli (50.70%), followed by Klebsiella pneumoniae (23.1%) and Enterococcus spp. (9.7%). Conclusions: the findings of this investigation reveal the intrinsic association between risk factors and microorganisms for the development of urinary tract infection.

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