Gene expression data and FTIR spectra provide a similar phenotypic description of breast cancer cell lines in 2D and 3D cultures

The Analyst ◽  
2018 ◽  
Vol 143 (11) ◽  
pp. 2520-2530 ◽  
Author(s):  
Margarita Smolina ◽  
Erik Goormaghtigh
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Expression Patterns ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Strongly Correlated ◽  
P Gene

Gene expression patterns and FTIR spectral data are strongly correlated. Both identified the genotypes and phenotypes of breast cancer cell lines.

scholarly journals Effects of Herceptin treatment on global gene expression patterns in HER2-amplified and nonamplified breast cancer cell lines

Oncogene ◽  
2003 ◽  
Vol 23 (4) ◽  
pp. 1010-1013 ◽  
Author(s):  
Päivikki Kauraniemi ◽  
Sampsa Hautaniemi ◽  
Reija Autio ◽  
Jaakko Astola ◽  
Outi Monni ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Expression Patterns ◽  
Global Gene Expression ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Gene Expression Patterns

scholarly journals Gene expression profiling of breast cancer cell lines treated with proton and electron radiations

2018 ◽  
pp. 20170934 ◽  
Author(s):  
Valentina Bravatà ◽  
Luigi Minafra ◽  
Francesco Paolo Cammarata ◽  
Pietro Pisciotta ◽  
Debora Lamia ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Gene Expression Profiling ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Expression Profiling ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines

scholarly journals Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines

2007 ◽  
Vol 29 (6) ◽  
pp. 467-476
Author(s):  
Joana Paredes ◽  
Ana Luísa Correia ◽  
Ana Sofia Ribeiro ◽  
Fernando Schmitt
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Expression Patterns ◽  
Cell Signalling ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
P120 Catenin ◽  
Transcriptional Phenotype

Background: P120-catenin is a member of the Armadillo protein family, which is involved in intercellular adhesion and cell signalling. It directly interacts with the classical cadherins juxtamembrane domain and contributes for both junction formation and its disassembly. Accumulating evidences indicate that p120-catenin is important in tumour formation and progression, although the role of their multiple spliced isoforms in the regulation of cadherin-mediated adhesion of malignant cells is still not well understood. We investigated the expression of p120-catenin isoforms in a collection of breast cancer cell lines with distinct molecular profiles and expressing different cadherins. Methods: We assessed the expression by RT-PCR and Western-blotting analysis. Results: We observed that the expression of p120-catenin isoforms was associated with the genomic and transcriptional phenotype of breast cancer cells. Besides, the recruitment of p120-catenin isoforms was not apparently related with the particular expression of E-, P- or N-cadherin. Conclusion: We demonstrate that mammary tumour cells exhibit a characteristic p120-catenin isoform expression profile, depending from their specific genomic and transcriptional properties. These particular expression patterns, combined with other regulatory proteins and in a specific cellular context, may explain how p120-catenin can either contribute to strength intercellular adhesions or instead to promote cell motility.

scholarly journals Human Arylamine N-Acetyltransferase 1 (NAT1) Knockout in MDA-MB-231 Breast Cancer Cell Lines Leads to Transcription of NAT2

2022 ◽  
Vol 12 ◽  
Author(s):  
Samantha M. Carlisle ◽  
Patrick J. Trainor ◽  
Mark A. Doll ◽  
David W. Hein
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Differential Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Global Gene Expression ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Metabolizing Enzyme

Many cancers, including breast cancer, have shown differential expression of human arylamine N-acetyltransferase 1 (NAT1). The exact effect this differential expression has on disease risk and progression remains unclear. While NAT1 is classically defined as a xenobiotic metabolizing enzyme, other functions and roles in endogenous metabolism have recently been described providing additional impetus for investigating the effects of varying levels of NAT1 on global gene expression. Our objective is to further evaluate the role of NAT1 in breast cancer by determining the effect of NAT1 overexpression, knockdown, and knockout on global gene expression in MDA-MB-231 cell lines. RNA-seq was utilized to interrogate differential gene expression (genes correlated with NAT1 activity) across three biological replicates of previously constructed and characterized MDA-MB-231 breast cancer cell lines expressing parental (Scrambled), increased (Up), decreased (Down, CRISPR 2–12), or knockout (CRISPR 2–19, CRISPR 5–50) levels of NAT1. 3,889 genes were significantly associated with the NAT1 N-acetylation activity of the cell lines (adjusted p ≤ 0.05); of those 3,889 genes, 1,756 were positively associated with NAT1 N-acetylation activity and 2,133 were negatively associated with NAT1 N-acetylation activity. An enrichment of genes involved in cell adhesion was observed. Additionally, human arylamine N-acetyltransferase 2 (NAT2) transcripts were observed in the complete NAT1 knockout cell lines (CRISPR 2–19 and CRISPR 5–50). This study provides further evidence that NAT1 functions as more than just a drug metabolizing enzyme given the observation that differences in NAT1 activity have significant impacts on global gene expression. Additionally, our data suggests the knockout of NAT1 results in transcription of its isozyme NAT2.

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