A novel mechanism for user-friendly and self-activated microdroplet generation capable of programmable control

The Analyst ◽  
2018 ◽  
Vol 143 (16) ◽  
pp. 3798-3807 ◽  
Author(s):  
Yangyang Jiang ◽  
Lin Du ◽  
Yuanming Li ◽  
Quanquan Mu ◽  
Zhongxu Cui ◽  
...  

The real-time continuous-flow PCR inside a 3D spiral microchannel is realized by a novel self-activated microdroplet generation/transport mechanism.

2017 ◽  
Vol 6 (10) ◽  
pp. 22551-22558
Author(s):  
BiswaRanjan Samal ◽  
Mrutyunjaya Panda

Whenever a feedback system comes into mind, it’s always a demand of the e-commerce organizations to get the customer feedbacks in real time and to build some strong dashboards on top of these feedbacks/ratings. So that they can easily know the performance of any product at any point of time as well as they could able to take a decision, on what to do with the products those are getting very poor feedbacks. Which will result in a minimum impact on the tangible and intangible assets of the organizations. For achieving the above goal it is very necessary for these organizations to adopt the right tool and implement the required environment which can deal with the real time big data ingestion, enrichment, indexing and have the power to perform simple as well as complex analysis algorithm on the stored data. In this paper, we have collected Amazon Product Ratings for doing analysis and used Apache NiFi for ingesting real-time data into Apache Solr and have taken help of Banana Dashboard to show the real time analysis results in the form of attractive and user-friendly dashboards.


Micromachines ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 350 ◽  
Author(s):  
Di Wu ◽  
Bing Shi ◽  
Bin Li ◽  
Wenming Wu

The self-activated micropump capable of velocity-stable transport for both single-phased plug and double-phased droplet through long flow distance inside 3D microchannel is one dream of microfluidic scientists. While several types of passive micropumps have been developed based on different actuation mechanisms, until today, it is still one bottleneck to realize such a satisfied self-activated micropump for the stable delivery of both single and double-phased liquid inside long microchannel (e.g., several meters), due to the lack of innovative mechanism in previous methods. To solve this problem, in this article, we propose a new self-activated pumping mechanism. Herein, an end-opened gas-impermeable quartz capillary is utilized for passive transport. Mechanism of this micropump is systemically studied by both the mathematical modeling and the experimental verifications. Based on the flow assays, it totally confirmed a different pumping principle in this paper, as compared with our previous works. The R 2 value of the overall flow rates inside the 3D microchannel is confirmed as high as 0.999, which is much more homogeneous than other passive pumping formats. Finally, this novel micropump is applied to continuous-flow real-time PCRs (both plug-type and microdroplet-type), with the amplification efficiency reaching 91.5% of the commercial PCR cycler instrument.


2014 ◽  
Author(s):  
Irving Biederman ◽  
Ori Amir
Keyword(s):  

2015 ◽  
Vol 2 (1) ◽  
pp. 35-41
Author(s):  
Rivan Risdaryanto ◽  
Houtman P. Siregar ◽  
Dedy Loebis

The real-time system is now used on many fields, such as telecommunication, military, information system, evenmedical to get information quickly, on time and accurate. Needless to say, a real-time system will always considerthe performance time. In our application, we define the time target/deadline, so that the system should execute thewhole tasks under predefined deadline. However, if the system failed to finish the tasks, it will lead to fatal failure.In other words, if the system cannot be executed on time, it will affect the subsequent tasks. In this paper, wepropose a real-time system for sending data to find effectiveness and efficiency. Sending data process will beconstructed in MATLAB and sending data process has a time target as when data will send.


Author(s):  
Jiyang Yu ◽  
Dan Huang ◽  
Siyang Zhao ◽  
Nan Pei ◽  
Huixia Cheng ◽  
...  

Author(s):  
Vladimir V. NEKRASOV

Developing a microcontroller-based system for controlling the flywheel motor of high-dynamics spacecraft using Russian-made parts and components made it possible to make statement of the problem of searching control function for a preset rotation rate of the flywheel rotor. This paper discusses one of the possible options for mathematical study of the stated problem, namely, application of structural analysis based on graph theory. Within the framework of the stated problem a graph was constructed for generating the new required rate, while in order to consider the stochastic case option the incidence and adjacency matrices were constructed. The stated problem was solved using a power matrix which transforms a set of contiguous matrices of the graph of admissible solution edge sequences, the real-time control function was found. Based on the results of this work, operational trials were run for the developed control function of the flywheel motor rotor rotation rate, a math model was constructed for the real-time control function, and conclusions were drawn about the feasibility of implementing the results of this study. Key words: Control function, graph, incidence matrix, adjacency matrix, power matrix, microcontroller control of the flywheel motor, highly dynamic spacecraft.


2017 ◽  
pp. 99-103
Author(s):  
Van Bao Thang Phan ◽  
Hoang Bach Nguyen ◽  
Van Thanh Nguyen ◽  
Thi Nhu Hoa Tran ◽  
Viet Quynh Tram Ngo

Introduction: Infection with HPV is the main cause of cervical cancer. Determining HPV infection and the types of HPV plays an important role in diagnosis, treatment and prognosis of cervicitis/cervical cancer. Aims: Determining proportion of high-risk HPV types and the occurrence of coinfection with multiple HPV types. Methods: 177 women with cervicitis or abnormal Pap smear result were enrolled in the study. Performing the real-time PCR for detecting HPV and the reverse DOT-BLOT assay for determining type of HPV in cases of positive PCR. Results: 7 types of high-risk HPV was dectected, the majority of these types were HPV type 18 (74.6%) and HPV type 16 (37.6%); the proportion of infection with only one type of HPV was 30.4% and coinfection with multiple HPV types was higher (69.6%), the coinfected cases with 2 and 3 types were dominated (32.2% and 20.3%, respectively) and the coinfected cases with 4 and 5 types were rare. Conclusion: Use of the real-time PCR and reverse DOT-BLOT assay can determine the high-risk HPV types and the occurrence of coinfection with multiple HPV types. Key words: HPV type, Reverse DOT-BLOT, real-time PCR,PCR, cervical cancer


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