scholarly journals Quantitative proteomic analysis of HeLa cells in response to biocompatible Fe2C@C nanoparticles: 16O/18O-labelling & HPLC-ESI-orbit-trap profiling approach

2018 ◽  
Vol 7 (1) ◽  
pp. 84-92 ◽  
Author(s):  
Murtaza Hasan ◽  
Ghazala Mustafa ◽  
Javed Iqbal ◽  
Muhammad Ashfaq ◽  
Nasir Mahmood
Keyword(s):  
Cell Culture ◽  
Hela Cells ◽  
Proteomic Analysis ◽  
Quantitative Proteomics ◽  
Molecular Response ◽  
Isotopic Labelling ◽  
Proteomics Analysis ◽  
Quantitative Proteomic Analysis

Here, we have investigated the comparative quantitative proteomics analysis of the molecular response of HeLa cells to biocompatible Fe2C@C nanoparticles (NPs) using 16O/18O isotopic labelling of the cell culture.

scholarly journals Quantitative proteomic analysis of cervical cancer based on TMT-labeled quantitative proteomics

2021 ◽  
pp. 104453
Author(s):  
Dianqin Xu ◽  
Xiaoyu Zhu ◽  
Ji Ren ◽  
Shan Huang ◽  
Ziwen Xiao ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Proteomic Analysis ◽  
Quantitative Proteomics ◽  
Quantitative Proteomic Analysis

scholarly journals iTRAQ-Based Quantitative Proteomics Analysis of HeLa Cells Infected With Chlamydia muridarum TC0668 Mutant and Wild-Type Strains

2019 ◽  
Vol 10 ◽  
Author(s):  
Yingzi Wang ◽  
Emmanuel Wirekoh Arthur ◽  
Na Liu ◽  
Xiaofang Li ◽  
Wenjing Xiang ◽  
...  
Keyword(s):  
Hela Cells ◽  
Quantitative Proteomics ◽  
Wild Type ◽  
Proteomics Analysis ◽  
Chlamydia Muridarum ◽  
Type Strains

scholarly journals Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherich coli biofilm formation in coculture

2020 ◽  
Author(s):  
Huiyi Song ◽  
Ni Lou ◽  
Jianjun Liu ◽  
Hong Xiang ◽  
Dong Shang
Keyword(s):  
Proteomic Analysis ◽  
Biofilm Formation ◽  
Quantitative Proteomics ◽  
Lactobacillus Rhamnosus ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Proteomics Analysis ◽  
Biofilm Inhibition ◽  
Sterile Culture

Abstract Background: Escherich coli (E.coli) is the principal pathogen that causes biofilm formation; the latter is associated with infectious diseases and antibiotic resistance. In our previous work, we demonstrated that probiotic microcapsules have superior biofilm inhibition capacity compared to probiotic sterile culture supernatant. Herein, the mechanism of the inhibition effects was investigated using label-free quantitative proteomics analysis. Results: The proteomic analysis characterized a total of 1655 proteins in E.coli K12MG1655 and 1431 proteins in Lactobacillus rhamnosus GG (LGG). Among them, after coculture treatment, there were 262 and differentially expressed proteins that were specific for E.coli and 291 for LGG. The differentially expressed proteins after coculture were related to cellular metabolism, the stress response, transcription, and the cell membrane. In addition, we identified five strain-specific genes in E.coli and LGG, respectively, which were consistent with the proteomics results. Conclusions: These findings indicate that LGG microcapsules may inhibit E.coli biofilm inhibition by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.

Quantitative proteomic analysis of MDCK cell adhesion

2021 ◽  
Author(s):  
Xuanqing Ye ◽  
Jiamin Wang ◽  
Zilin Qiao ◽  
Di Yang ◽  
Jiao Wang ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Genetic Engineering ◽  
Cell Line ◽  
Mdck Cell ◽  
Proteomic Analysis ◽  
Quantitative Proteomics ◽  
Mdck Cells ◽  
Vaccine Production ◽  
Quantitative Proteomic Analysis ◽  
Suspension Cell Line

Establishing a stable MDCK suspension cell line by genetic engineering has significant potential to aid industrialization of vaccine production. In this study, quantitative proteomics was used to explore adhesion proteins in MDCK cells.

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