scholarly journals Regulating temperature and relative humidity in air–liquid interface in vitro systems eliminates cytotoxicity resulting from control air exposures

2017 ◽  
Vol 6 (4) ◽  
pp. 448-459 ◽  
Author(s):  
Jose Zavala ◽  
Rebecca Greenan ◽  
Q. Todd Krantz ◽  
David M. DeMarini ◽  
Mark Higuchi ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Liquid Interface ◽  
Humidity Control ◽  
Exposure System ◽  
Temperature And Humidity ◽  
In Vitro Systems ◽  
Air Liquid Interface

Modifications to a VITROCELL exposure system were required to mitigate cytotoxicity caused by the absence of temperature and humidity control.

scholarly journals Parametric Optimization of an Air–Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeO2 Nanoparticles

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2369 ◽  
Author(s):  
Lars Leibrock ◽  
Harald Jungnickel ◽  
Jutta Tentschert ◽  
Aaron Katz ◽  
Blaza Toman ◽  
...  
Keyword(s):  
Co2 Concentration ◽  
Liquid Interface ◽  
In Vivo Studies ◽  
Inhalation Toxicity ◽  
Exposure System ◽  
Inhalation Study ◽  
Flow Through ◽  
Air Liquid Interface

Air–liquid interface (ALI) systems have been widely used in recent years to investigate the inhalation toxicity of many gaseous compounds, chemicals, and nanomaterials and represent an emerging and promising in vitro method to supplement in vivo studies. ALI exposure reflects the physiological conditions of the deep lung more closely to subacute in vivo inhalation scenarios compared to submerged exposure. The comparability of the toxicological results obtained from in vivo and in vitro inhalation data is still challenging. The robustness of ALI exposure scenarios is not yet well understood, but critical for the potential standardization of these methods. We report a cause-and-effect (C&E) analysis of a flow through ALI exposure system. The influence of five different instrumental and physiological parameters affecting cell viability and exposure parameters of a human lung cell line in vitro (exposure duration, relative humidity, temperature, CO2 concentration and flow rate) was investigated. After exposing lung epithelia cells to a CeO2 nanoparticle (NP) aerosol, intracellular CeO2 concentrations reached values similar to those found in a recent subacute rat inhalation study in vivo. This is the first study showing that the NP concentration reached in vitro using a flow through ALI system were the same as those in an in vivo study.

scholarly journals In vitro toxicological evaluation of emissions from catalytic oxidation removal of industrial VOCs by air/liquid interface (ALI) exposure system in repeated mode

2019 ◽  
Vol 58 ◽  
pp. 110-117 ◽  
Author(s):  
Clémence Méausoone ◽  
Rebecca El Khawaja ◽  
Gauthier Tremolet ◽  
Stéphane Siffert ◽  
Renaud Cousin ◽  
...  
Keyword(s):  
Catalytic Oxidation ◽  
Liquid Interface ◽  
Toxicological Evaluation ◽  
Exposure System ◽  
Air Liquid Interface

Zwischen Luft und Wasser – Air-Liquid-Interface-Kulturen als In-vitro-Modell des Respirationstrakts

2020 ◽  
Author(s):  
S. Runft ◽  
L. Burigk ◽  
A. Lehmbecker ◽  
K. Schöne ◽  
D. Waschke ◽  
...  
Keyword(s):  
Liquid Interface ◽  
Air Liquid Interface
Sign in / Sign up

Export Citation Format

Share Document